&lt;p&gt;LINC01296 promotes proliferation, migration, and invasion of HCC cells by targeting miR-122-5P and modulating EMT activity&lt;/p&gt;

Wan, Yiming; Li, Molin; Huang, Ping

doi:10.2147/ott.s197338

Cited by 15 publications

(13 citation statements)

References 42 publications

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“…Many studies have reported that lncRNAs can interact with miRNA to exert their regulatory functions [12,13]. For example, LINC01296 promotes HCC cells proliferation, migration, and invasion through targeting miR-122-5p [14]. Long noncoding RNA SNHG1 promotes non-small cell lung cancer progression via sponging miR-145-5p [15].…”

Section: Discussionmentioning

confidence: 99%

RNF185-AS1 Promotes Hepatocellular Carcinoma Progression Through Targeting miR-221-5p/integrin β5 Axis

Huang

et al. 2020

Preprint

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Background: Recently, long noncoding RNAs (lncRNAs) have been reported to play important role in pathogenesis of various cancers. However, the function of RNF185-AS1 in hepatocellular carcinoma (HCC) metastasis has not been well investigated. The present study aims to explore the role and mechanism of RNF185-AS1 in hepatocellular carcinoma metastasis. Methods: The RNF185-AS1 expression in HCC cells and tissues was measured by quantitative real‐time polymerase chain reaction (qRT-PCR). The functional effects of RNF185-AS1 on tumor cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) were assessed by Cell Counting Kit-8 (CCK8) assay, colony formation assay, transwell assay and Western blot. The luciferase reporters assay, RNA-binding protein immunoprecipitation assay, qRT-PCR and Western blot were performed to explore and confirm the interaction between RNF185-AS1 and miR-221-5p and integrin β5. The role of RNF185-AS1 in tumor progression was explored through in vivo experiments.Results: RNF185-AS1 was highly expressed in HCC tissues and cell lines. High levels of RNF185-AS1 was correlated with advanced TNM stage, distant metastasis and a poorer overall survival rate. RNF185-AS1 knockdown inhibited cell proliferation, migration, invasion and EMT. Additionally, RNF185-AS1 acted as a sponge for miR-221-5p and integrin β5 was identified as a target gene of miR-221-5p. Rescue assays showed that miR-221-5p inhibitor or integrin β5 overexpression rescued the function of RNF185-AS1 knockdown on cell proliferation, migration, invasion and EMT. Moreover, we found that RNF185-AS1 knockdown inhibited tumor metastases in xenograft tumor mouse model. Conclusion: Our findings demonstrated that RNF185-AS1 promoted cell EMT and migration by regulating miR-221-5p/integrin β5 axis in HCC.

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Section: Discussionmentioning

confidence: 99%

RNF185-AS1 Promotes Hepatocellular Carcinoma Progression Through Targeting miR-221-5p/integrin β5 Axis

Huang

et al. 2020

Preprint

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“…A total of nine eligible studies21–25 28 31–33 (one gastric cancer, one oesophageal cancer, one prostate cancer, one cholangiocarcinoma, one colon cancer, one breast cancer, one pancreatic ductal adenocarcinoma, one bladder cancer and one hepatocellular carcinoma) were enrolled in this meta-analysis. The basic information and characteristics of each trial were summarised in tables 1–3.…”

Section: Resultsmentioning

confidence: 99%

“…In this meta-analysis, the expression level of LINC01296 was examined by qRT-PCR in all studies. The median expression of LINC01296 in Qin’s,22 Wang’s28 and Wan’s33 articles was regarded as the cut-off value, while the mean expression was set as the cut-off value in Zhang’s,23 Yuan’s25 and Wang’s32 researches. However, other eligible articles did not report the cut-off value.…”

Section: Resultsmentioning

confidence: 99%

“…As mentioned above, an electronic database search identified 113 literatures according to the search strategy. After a full assessment of these researches, a total of 11 articles21–26 28 31–34 that reported the possible functions or pathways of LINC01296 in human solid malignant tumours were included. Following a summary of the functions and pathways of LINC01296 among the articles, we showed a concise overview of the functionality of LINC01296 in figure 6.…”

Section: Resultsmentioning

confidence: 99%

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Prognostic value of long non-coding RNA 01296 expression in human solid malignant tumours: a meta-analysis

Dai

et al. 2019

Postgraduate Medical Journal

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Long intergenic non-coding RNA 01296 (LINC01296) has been reported to play an important role in many human malignancies, but a consistent perspective has not been established now. To explore the prognostic value of LINC01296 in different types of human solid malignant tumours, we performed this meta-analysis.An electronic search of PubMed, EMBASE, Web of Science, China National Knowledge Infrastructure, Cochrane Library, Chinese Biological Medical Literature database and WanFang database was applied to select eligible literatures. Pooled ORs or HRs with their 95% CIs were calculated to estimate the effects.A total of 559 patients from nine eligible studies were enrolled in this meta-analysis. The results revealed that high LINC01296 expression was significantly related to larger tumour size (OR 3.42, 95% CI 2.08 to 5.63), lymph node metastasis (OR 3.03, 95% CI 2.01 to 4.57) and advanced tumor-node-metastasis (TNM) stage (OR 4.41, 95% CI 2.65 to 7.34). Moreover, we found that elevated LINC01296 expression predicted a poor outcome for overall survival (HR 1.78, 95% CI 1.48 to 2.14) and recurrence-free survival (HR 4.00, 95% CI 1.04 to 15.67).High expression levels of LINC01296 were associated with unfavourable clinical outcomes of patients with cancer. Our results indicated that LINC01296 could serve as a prognostic predictor in human solid malignant tumours.

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“…In colon carcinoma, miR-21a combines with LINC01296 to reverse its carcinogenic effect [12]. In addition, LINC01296/miR-122-5p axis regulate the development of hepatocellular carcinoma through EMT pathway [13]. These findings provided an insight for us to explore how LINC01296 implicated in the progression of OC.…”

Section: Introductionmentioning

confidence: 90%

MiR-29c-3p, a target miRNA of LINC01296, accelerates tumor malignancy: therapeutic potential of a LINC01296/miR-29c-3p axis in ovarian cancer

Mao

Zhao

et al. 2020

J Ovarian Res

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As one of the main gynecological cancers, ovarian cancer (OC) has an unfavourable outcomes owing to its high recurrence and metastasis rate. Our previous studies have revealed that LINC01296 functions as an oncogene in OC, but the underlying mechanism has not been explored. The aim of this paper was to further investigate that how LINC01296 plays a role in OC. Through online software prediction, miR-29c-3p has been discriminated as the target miRNA of LINC01296 for further research, and subsequent luciferase assay confirmed bioinformatics prediction. Then the data obtained from the two databases (GSE119055 and GSE83693) were analyzed by GEO2R for differential gene analysis. The results indicated that the miR-29c-3p was lowly expressed in OC tissues than that in normal ovarian tissues, and its expression in recurrent OC tissues was lower than that in primary OC tissues. Simultaneously, Kaplan-Meier survival analysis illustrated that the lower expression of miR-29c-3p was interrelated to unfavourable outcomes of OC. Further, the qRT-PCR data revealed that the miR-29c-3p expression in OC cell lines (SKOV-3 and OVCAR-3) was markedly declined than that in normal control cells (IOSE80). Subsequently, the functional experiments, such as CCK8, colony formation and Transwell assays, prompted that inhibition of miR-29c-3p can obviously increase the proliferation, invasion and migration of OVCAR3 and SKOV3 cells compared with control group, while downregulation of LINC01296 showed an opposite result. It is worth noting that downregulation of LINC01296 can reverse the effect of miR-29c-3p suppression on OC cells. Finally, we detected the changes of EMT-related proteins by western blot experiment, and reached a similar conclusion that knockdown of LINC01296 reversed the EMT caused by miR-29c-3p inhibition. In sum up, the cancer-promoting function of LINC01296 was achieved by regulating the expression of miR-29c-3p, and LINC01296/miR-29c-3p axis mediates the mechanical regulation of EMT in OC cells, hoping to provide the novel biomarkers and possibilities for OC therapy.

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<p>LINC01296 promotes proliferation, migration, and invasion of HCC cells by targeting miR-122-5P and modulating EMT activity</p>

Cited by 15 publications

References 42 publications

RNF185-AS1 Promotes Hepatocellular Carcinoma Progression Through Targeting miR-221-5p/integrin β5 Axis

RNF185-AS1 Promotes Hepatocellular Carcinoma Progression Through Targeting miR-221-5p/integrin β5 Axis

Prognostic value of long non-coding RNA 01296 expression in human solid malignant tumours: a meta-analysis

MiR-29c-3p, a target miRNA of LINC01296, accelerates tumor malignancy: therapeutic potential of a LINC01296/miR-29c-3p axis in ovarian cancer

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