<p>LncRNA CTBP1-AS2 Facilitates Gastric Cancer Progression via Regulating the miR-139-3p/MMP11 Axis</p>

Abstract

Background This study aimed at probing into the effect of long non-coding RNA (lncRNA) C-terminal binding protein 1 antisense RNA 2 (CTBP1-AS2) on gastric cancer (GC) cell proliferation and apoptosis, and its regulatory function on miR-139-3p and MMP11 . Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to examine the expressions of CTBP1-AS2, miR-139-3p and MMP11 mRNA in GC cell lines and clinical specimens. Cell counting kit-8 (CCK-8) assay, flow cytometry and EdU assay were conducted to examine the effects of CTBP1-AS2 and miR-139-3p on GC cell proliferation and apoptosis. Western blot was applied for detecting the expressions of Bax, Bcl-2 and MMP11 . A lung metastasis mouse model was used to evaluate metastasis of GC cells in vivo. Bioinformatics, dual-luciferase report assay, RIP and RNA pull-down assays were utilized to validate the targeted relationship between CTBP1-AS2 and miR-139-3p as well as the targeting relationship between miR-139-3p and MMP11 . Results CTBP1-AS2 was highly expressed in GC, and its high expression was strongly associated with increased TNM stage, increased tumor size and low degree of differentiation of the tumor tissues. Meanwhile, CTBP1-AS2 promoted GC cell proliferation, metastasis and suppressed apoptosis, while miR-139-3p could weaken these effects. In addition, CTBP1-AS2 was identified as a molecular sponge for miR-139-3p, and MMP11 was verified as a target gene of CTBP1-AS2. CTBP1-AS2 could increase the expression of MMP11 via repressing miR-139-3p. Conclusion CTBP1-AS2 promotes GC cells and inhibits apoptosis by regulating the miR-139-3p/ MMP11 molecular axis.

Low expression of lncRNA APTR promotes gastric cancer progression

“…For instance, lncRNA CTBP1-AS2 has been shown to promote hepatocellular carcinoma cell proliferation by sponging miR-623 [23] and miR-195–5p [24] and facilitate colorectal cancer development by inhibiting miR-93–5p [25] . As in gastric cancer, CTBP1-AS2 was shown to increase MMP11 expression via suppressing miR-139–3p [26] , leading to gastric cancer progression. In our study, we found lncRNA APTR is significantly lower expressed in gastric tumor tissues and its expression in gastric cancer is negatively correlated with tumor grade, stage, invasion, and metastasis.…”

Long non‑coding RNA CTBP1‑AS2 upregulates USP22 to promote pancreatic carcinoma progression by sponging miR‑141‑3p

“…Specifically, CTBP1-AS2 can facilitate hepatocellular carcinoma cell proliferation by regulating the miR-623/Cyclin D1 axis ( 9 ). In gastric cancer, CTBP1-AS2 promotes cells proliferation and metastasis and suppresses apoptosis by regulating the miR-139-3p/MMP11 axis ( 17 ). CTBP1-AS2 expression is upregulated in osteoarthritis and increases the methylation of miR-130a gene to suppress the proliferation of chondrocytes ( 18 ).…”

The Intricate Interplay between the ZNF217 Oncogene and Epigenetic Processes Shapes Tumor Progression

“…Altogether, this study was pioneering in demonstrating the oncogenic role of CTBP1-AS2 in cancer progression and in discovering a new CTBP1-AS2/miR-3163/ZNF217 regulatory network. Since this study, recent works conducted in other cancers validated the oncogenic function of lncRNA CTBP1-AS2 [ 94 , 95 , 96 ]. Importantly, one study discovered that lncRNA CTBP1-AS2 was able to modulate the TGF-β/SMAD2/3 pathway [ 94 ].…”