<p>LncRNA MAGI2-AS3 Affects Cell Invasion and Migration of Cervical Squamous Cell Carcinoma (CSCC) via Sponging miRNA-233/EPB41L3 Axis</p>

Abstract

Introduction: The incidence of cervical squamous cell carcinoma (CSCC) has expanded in recent years. However, the function of long non-coding RNA (lncRNA) MAGI2-AS3 in the occurrence and progression of CSCC remains unclear. Therefore, the role of lncRNA MAGI2-AS3 in cervical squamous cell carcinoma (CSCC) was investigated in our study. Methods: We used qRT-PCR analysis to identify the level of MAGI2-AS3 mRNA expression in CSCC clinical samples and cell lines. We investigated cell migration and invasion of CSCC cells transfected with MAGI2-AS3, miR-233 mimic, or EPB41L3 with transwell assays. Bioinformatics analysis and a luciferase reporter assay were employed to predict the interaction between MAGI2-AS3 and miR-233. Results: We found that MAGI2-AS3 and EPB41L3 were both downregulated in CSCC and the expression of this two was positively correlated. Bioinformatics analysis showed that MAGI2-AS3 might bind to miR-233, which could directly target EPB41L3. In CSCC cells, overexpression of MAGI2-AS3 led to upregulated, while overexpression of miRNA-233 led to downregulated expression of EPB41L3. However, MAGI2-AS3 and miR-233 did not affect the expression of each other. In addition, overexpression of MAGI2-AS3 and EPB41L3 led to inhibited cancer cell invasion and migration, while overexpression of miR-233 played an opposite role and attenuated the effects of overexpressing MAGI2-AS3. Conclusion: MAGI2-AS3 may sponge miR-233 to upregulate EPB41L3, thereby inhibiting CSCC cell invasion and migration.

Histone deacetylase 10 exerts antitumor effects on cervical cancer via a novel microRNA‐223/TXNIP/Wnt/β‐catenin pathway

“…Intriguingly, miR-223 was highly expressed in CC tissues, 15 and the increased expression of miR-223 in CC tissues was possibly modulated by the transcription factor STAT3. 16 Our prediction using the UCSC bioinformatics website and ChIP assay revealed that HDAC10 bound to the miR-223 promoter and positively regulated its acetylation level, indicating a novel acetylation-dependent regulation of miR-223 upregulation in CC.…”

Role of lncRNA MAGI2‐AS3 in lipopolysaccharide‐induced nucleus pulposus cells injury by regulating miR‐374b‐5p/interleukin‐10 axis

“…LncRNA MAGI2‐AS3 is a recently identified lncRNA, which has been widely examined as a tumor suppressor in various cancers, including hepatocellular carcinoma, bladder, lung, ovarian, cervical, and prostate cancers. 29 , 30 , 31 , 32 , 33 , 34 LncRNA MAGI2‐AS3 plasma levels have been evaluated in patients with IDD pre‐ and post‐treatment, and the findings indicated that patients with IDD were found to exhibit reduced lncRNA MAGI2‐AS3 expression, and its expression was significantly augmented after clinical treatments. 15 Herein, our results confirmed that lncRNA MAGI2‐AS3 expression was reduced in LPS‐triggered NP cells, and augmenting this expression could promote NP cell viability and ECM synthesis while suppressing apoptosis and inflammation.…”

“…LncRNA MAGI2‐AS3 is a recently identified lncRNA, which has been widely examined as a tumor suppressor in various cancers, including hepatocellular carcinoma, bladder, lung, ovarian, cervical, and prostate cancers 29–34 . LncRNA MAGI2‐AS3 plasma levels have been evaluated in patients with IDD pre‐ and post‐treatment, and the findings indicated that patients with IDD were found to exhibit reduced lncRNA MAGI2‐AS3 expression, and its expression was significantly augmented after clinical treatments 15 .…”

A New Story of the Three Magi: Scaffolding Proteins and lncRNA Suppressors of Cancer

“…In this study, the prognostic value of MAGI2-AS3 was not statistically significant. Similarly, Hou et al evidenced a decreased accumulation of MAGI2-AS3 in cervix squamous cell carcinomas compared with control tissue [ 215 ]. They further demonstrated that MAGI2-AS3 inhibited SiHa and HeLa cell invasion and migration by sponging miR-223 leading to up-regulation of the tumor suppressor EPB41L3.…”