<p>Long Non-Coding RNA CDKN2B-AS1 Facilitates Laryngeal Squamous Cell Cancer Through Regulating miR-497/CDK6 Pathway</p>

Abstract

Background: CDKN2B antisense RNA 1 (CDKN2B-AS1), a long noncoding RNA, was reported to play crucial roles in the progression of multiple cancers. However, the functional roles and regulatory mechanism of CDKN2B-AS1 in human laryngeal squamous cell cancer (LSCC) remain unclear. The goals of this study were to investigate biological roles and underlying mechanisms of CDKN2B-AS1 in LSCC. Methods: The expressions of CDKN2B-AS1, miR-497 and cyclin-dependent kinase 6 (CDK6) were detected in LSCC tissues and cell lines by real-time quantitative PCR (qRT-PCR). The effects of CDKN2B-AS1 on LSCC cell proliferation, apoptosis, migration and invasion were examined by corresponding experiments. Bioinformatics analysis and luciferase activity assay were applied to analyze the interaction between CDKN2B-AS1 and miR-497. Results: The expression of CDKN2B-AS1 was significantly higher in LSCC tissues than in adjacent normal tissues. Higher CDKN2B-AS1 was closely associated with lymph node metastasis and advanced clinical stage. Moreover, CDKN2B-AS1 knockdown by siRNA significantly inhibited the proliferation, induced cell apoptosis, and suppressed migration and invasion in LSCC cells. Mechanically, CDKN2B functions as an oncogenic lncRNA in LSCC via regulating miR-497/CDK6 axis. Conclusion: The observations in this study identify CDKN2B-AS1 an oncogenic role in the tumorigenesis of LSCC by regulating miR-497/CDK6 axis and indicate that it may serve as a potential target for LSCC treatment.

LncRNA CDKN2B-AS1/miR-141/cyclin D network regulates tumor progression and metastasis of renal cell carcinoma

“…CDKN2B-AS1 , also known as ANRIL , is located at chromosome 9p21. CDKN2B-AS1 is reported to be upregulated in tumor tissues and function as an oncogenic lncRNA in pancreatic, ovarian and laryngeal squamous cell carcinoma 36 – 38 . Human miR-141 is located at chromosome 12p13.31 and is transcribed from a miR-200 family cluster.…”

Regulation of microRNA‑497 expression in human cancer (Review)

“…It has been reported that the expression of this lncRNA is upregulated in tumor tissues and plays a carcinogenic role. A previous study has reported that CDKN2B-AS1 acts as an oncogene in the tumorigenesis of laryngeal squamous cell carcinoma by regulating the miR-497/cyclin-dependent kinase 6 axis ( 96 ).…”

<p>LncRNA CDKN2B-AS1 Promotes Cell Viability, Migration, and Invasion of Hepatocellular Carcinoma via Sponging miR-424-5p</p>

“…lncRNA CDKN2B antisense RNA 1 (CDKN2B-AS1), which is an antisense of the cyclin-dependent kinase inhibitor 2B (CDKN2B), plays important roles in various diseases including in cancers [15][16][17][18] . Zhu et al reported that interference of CDKN2B-AS1 restrains the metastasis and promotes apoptosis and senescence of cervical cancer cells via regulating miR-181a-5p/TGFβI axis.…”