<p>Long non-coding RNA homeobox A11 antisense RNA (HOXA11-AS) promotes retinoblastoma progression via sponging miR-506-3p</p>

Abstract

Background: Long non-coding RNA homeobox A11 antisense RNA (HOXA11-AS) has been reported to be involved in initiation and development of multiple cancers. However, the detailed biological roles and underlying molecular mechanism of HOXA11-AS remain unclear in retinoblastoma (RB). Herein, the goals of this study were to explore the biological function and the potential mechanism of HOXA11-AS in RB. Materials and methods: The expression of HOXA11-AS in RB tissues and cell lines was detected using real-time PCR (qRT-PCR). Cell proliferation, cycle arrest and apoptosis were measured using a cell counting kit 8 and flow cytometry. The target miRNAs of HOXA11-AS was predicted by Starbase2.0 software and was confirmed using a dual-luciferase reporter assay. Result: We found that HOXA11-AS expression was markedly elevated in RB tissues and cell lines compared to normal retina tissues and human retinal epithelial cells, respectively. Functional analysis showed that knockdown of HOXA11-AS in RB cells significantly suppressed cell proliferation, and induced cell cycle arrest at G1/G0 phase and promoted cell apoptosis. We also found that HOXA11-AS could serve as a competing endogenous RNA (ceRNA) that inhibited miR-506-3p expression, which regulated its downstream target NIMA-related kinase 6 (NEK6) in RB. In addition, miR-506-3p inhibitors partially reversed the effect of HOXA11-AS depletion on proliferation, cycle arrest and apoptosis in RB cells. Conclusion: Taken together, these findings demonstrated that HOXA11-AS could promote RB progression by sponging miR-506-3p, suggesting that HOXA-11-AS might be a potential therapeutic target for RB.

Circ_100565 promotes proliferation, migration and invasion in non-small cell lung cancer through upregulating HMGA2 via sponging miR-506-3p

“…i The correlation between miR-506-3p and circ_100565 expression was measured by Pearson correlation analysis. *P < 0.05, **P < 0.01, ***P < 0.001 discovered that it was also lower expressed in NSCLC, which was consistent with its expression in other cancers [16][17][18]. Besides, HMGA2, a well-known oncogene, could be targeted by miR-506-3p.…”

“…In NSCLC, miR-217, miR-600 and miR-488-3p have also been shown to be involved in the regulation of cir-cRNAs on NSCLC progression [8,14,15]. MiR-506-3p has been shown to be under-expressed in a variety of cancers, and can act as a tumor inhibitor to participate in the regulatory process of cancer, such as prostate cancer, retinoblastoma and osteosarcoma [16][17][18]. However, its function in NSCLC is still unclear.…”

Long non‑coding RNA NR2F1‑AS1 facilitates the osteosarcoma cell malignant phenotype via the miR‑485‑5p/miR‑218‑5p/BIRC5 axis

“…In hepatocellular carcinoma, DLGAP1-AS1 has been confirmed to facilitate cell proliferation via the miR-486-5p/H3F3B axis (35). NR2F1-AS1 has been reported to play a role in papillary thyroid carcinoma (14), ESCC (12), endometrial cancer (15), hepatocellular carcinoma (36) and OS (16). In OS, NR2F1-AS1 has been identified to sponge miR-483-3p to upregulate FOXA1 and enhance the malignant properties of OS cells (16).…”

“…Numerous studies have shown that lncRNAs have a wide range of functions in almost all physiologic processes of an organism, and they do not only regulate cell differentiation (6,7) and metabolism (8), but also participate in a variety of pathological processes, such as cancer proliferation, invasion, metastasis (9), apoptosis (10) and drug resistance (11). NR2F1-AS1, a recently discovered lncRNA, has been found to enhance esophageal squamous cell carcinoma (ESCC) progression by regulating the Hedgehog signaling pathway (12). NR2F1-AS1 has also been reported to regulate the microRNA (miR)-338-3p/cyclin D1 axis to promote thyroid cancer progression (13).…”

Checking NEKs: Overcoming a Bottleneck in Human Diseases

“…A recent study showed that NEK6 is regulated by long non-coding RNA homeobox A11 antisense RNA (HOXA11-AS) through MiR-506-3p in retinoblastoma [154]. HOXA11-AS can decrease MiR-506-3p expression.…”