&lt;p&gt;Long Non-Coding RNA PART1 Exerts Tumor Suppressive Functions in Glioma via Sponging miR-190a-3p and Inactivation of PTEN/AKT Pathway&lt;/p&gt;

Jin, Zheng Gen; Piao, Lianhua; Sun, Guangchao; Lv, Chuanxiang; Yi, Jing; Jin, Rihua

doi:10.2147/ott.s232848

Cited by 34 publications

(26 citation statements)

References 39 publications

(39 reference statements)

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“…In detail, PART1 acted as oncogenic factors in bladder cancer 32 and hepatocellular carcinoma, 33 while it acted as tumor suppressors in oral squamous cell carcinoma 22 and glioblastoma. 34,35 However, the biological effects and underlying mechanisms of PART1 on TSCC were unclear. In our study, we found that the expression levels of lncRNA PART1 and miR-503-5p were inversely correlated with each other in TSCC tissues, and overexpression of PART1 in TSCC cell lines inhibited miR-503 expression.…”

Section: Discussionmentioning

confidence: 99%

“…37 PART1 could mediate the secretion of cytokines and metastasis of tumor cells to affect cancer progression. 15 The expression of PART1 was impeded in glioma tissues and exerted suppressive functions on malignant behavior by targeting miR-190a-3p in glioma cell lines.- 35 Similarly, PART1 was down-regulated in TSCC tissues of patients and a higher level of PART1 was positively correlated with better prognosis. 15 Different miRNAs showed conversed effects in TSCC, as well.…”

Section: Discussionmentioning

confidence: 99%

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<p>LncRNA PART1 Exerts Tumor-Suppressive Functions in Tongue Squamous Cell Carcinoma via miR-503-5p</p>

Zhao

Yanqing

Cheng

et al. 2020

OTT

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Background: Tongue squamous cell carcinoma (TSCC) accounts for one-third of oral cancers. Previous studies had reported that lncRNA/miRNA regulated the biological behaviors of different cancer cells. However, the mechanisms of PART1 in regulating tumorigenesis and TSCC development via targeting miR-503-5p had not been studied. Methods: The expressions of PART1 and miR-503-5p in tissues and cultured cell lines were detected by qRT-PCR. StarBase 3.0 was used to predict the binding sites of PART1, then dual-luciferase assay and RNA pull-down assay were executed to confirm whether miR-503-5p was a target of PART1. TSCC cells were co-transfected with PART1-overexpressed plasmid or miR-503-5p mimics in vitro, and the transfection efficiency was evaluated through qRT-PCR. Western blot was performed to assess the expressions of EMT-related proteins. CCK-8 and clone formation assays were conducted to detect cell proliferation, TUNEL assay was used to detect apoptosis, and transwell assay was executed to test migration and invasion. Results: The low PART1 expression and high miR-503-5p expression were found in TSCC tissues and cell lines (CAL-27 and SCC9). PART1 expression was positively correlated with patients' prognosis. The targeting and binding relationship between PART1 and miR-503-5p was confirmed, and overexpressed PART1 diminished the expression of miR-503-5p as well. Moreover, PART1 facilitated apoptosis, inhibited proliferation, invasion and migration of TSCC cells, and these influences were impeded by miR-503-5p overexpression. Conclusion: LncRNA PART1 played a cancer-suppressing role in TSCC by targeting miR-503-5p, which provided a potential target for TSCC treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

<p>LncRNA PART1 Exerts Tumor-Suppressive Functions in Tongue Squamous Cell Carcinoma via miR-503-5p</p>

Zhao

Yanqing

Cheng

et al. 2020

OTT

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“…Previous studies have shown that PART1 plays a tumor suppressive function in gliomas [35], but has a procancer effect in bladder cancer [36], non-small-cell lung cancer [37], and colorectal cancer [38]. hsa-miRNA-429 plays an inhibitory role in a number of tumors [39][40][41], including glioma [42][43][44].…”

Section: Discussionmentioning

confidence: 99%

PART1 and hsa-miR-429-Mediated SHCBP1 Expression Is an Independent Predictor of Poor Prognosis in Glioma Patients

Xuan¹,

Jin²,

Wang³

et al. 2020

BioMed Research International

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Gliomas are the most common primary brain tumors. Because of their high degree of malignancy, patient survival rates are unsatisfactory. Therefore, exploring glioma biomarkers will play a key role in early diagnosis, guiding treatment, and monitoring the prognosis of gliomas. We found two lncRNAs, six miRNAs, and nine mRNAs that were differentially expressed by analyzing genomic data of glioma patients. The diagnostic value of mRNA expression levels in gliomas was determined by receiver operating characteristic (ROC) curve analysis. Among the nine mRNAs, the area under the ROC curve values of only CEP55 and SHCBP1 were >0.7, specifically 0.834 and 0.816, respectively. Additionally, CEP55 and SHCBP1 were highly expressed in glioma specimens and showed increased expression according to the glioma grade, and outcomes of high expression patients were poor. CEP55 was enriched in the cell cycle, DNA replication, mismatch repair, and P53 signaling pathway. SHCBP1 was enriched in the cell cycle, DNA replication, ECM receptor interaction, and P53 signaling pathway. Age, grade, IDH status, chromosome 19/20 cogain, and SHCBP1 were independent factors for prognosis. Our findings suggest the PART1-hsa-miR-429-SHCBP1 regulatory network plays an important role in gliomas.

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“…A previous study has identified a binding site on lncRNA PART1 for miR-190a-3p ( 26 ). Therefore, in the present study, Starbase version 2.0 ( http://starbase.sysu.edu.cn/ ) was used to verify the potential interaction between lncRNA PART1 and miR-190a-3p, which was confirmed using Dual-luciferase reporter assay.…”

Section: Methodsmentioning

confidence: 99%

“…miRNAs serve vital roles in numerous diseases by regulating gene expression, cell proliferation, inflammation, apoptosis and invasion (22)(23)(24), such that miRNA dysregulation has been previously associated with a number of human diseases (25). A recent study also revealed that miR-190a-3p is involved in glioma (26), where it was indicated that lncRNA PART1 exerts tumor suppressive effects by sponging miR-190a-3p, suggesting a regulatory effect of lncRNA PART1 on miR-190a-3p function (26). However, the role of miR-190a-3p in IDD progression is not completely understood and whether lncRNA PART1 can affect IDD by regulating miR-190a-3p remains unclear.…”

Section: Role Of Lncrna Part1 In Intervertebral Disc Degeneration Andmentioning

confidence: 99%

Role of lncRNA PART1 in intervertebral disc degeneration and associated underlying mechanism

et al. 2020

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Intervertebral disc degeneration (IDD) is a chronic skeletal muscle degeneration disease. Previous studies have demonstrated that long non-coding RNAs (lncRNAs) exert significant roles in serious illnesses. Prostate androgen-regulated transcript 1 (PART1) is an identified lncRNA that has been reported to be a regulator in a number of diseases. However, the potential effects of PART1 in IDD have yet to be fully elucidated. The present study aimed to investigate the roles of lncRNA PART1 in IDD and identify a possible underlying mechanism. Human nucleus pulposus (NP) cells were first exposed to lipopolysaccharide (LPS) to construct in vitro IDD models. Reverse transcription-quantitative PCR (RT-qPCR) was performed to measure lncRNA PART1 expression levels in 10 ng/ml LPS-stimulated NP cells and normal cells (untreated cells). Dual-luciferase reporter assays were conducted to verify the possible binding sites of microRNA (miR)-190a-3p on lncRNA PART1. In addition, NP cell viability and apoptosis were measured by performing MTT and flow cytometry, respectively. Expression and secretion of inflammatory factors (TNF-α, IL-1β and IL-6) and extracellular matrix (ECM) degradation-related proteins (aggrecan and collagen type II) were measured using ELISA, RT-qPCR and western blotting. Expression levels of lncRNA PART1 in LPS-treated NP cells were found to be higher compared with those in the control groups. miR-190a-3p directly targeted lncRNA PART1. PART1 knockdown enhanced cell viability, reduced cell apoptosis, inhibited inflammatory factor secretion and promoted ECM degradation in LPS-stimulated NP cells. However, transfection with the miR-190a-3p inhibitor reversed the aforementioned PART1 knockdown-induced alterations in cell viability, apoptosis, inflammatory cytokine and ECM degradation. Collectively, these results suggest that PART1 accelerates the progression of IDD by directly targeting miR-190a-3p, which provides a novel target for IDD diagnosis and treatment.

show abstract

<p>Long Non-Coding RNA PART1 Exerts Tumor Suppressive Functions in Glioma via Sponging miR-190a-3p and Inactivation of PTEN/AKT Pathway</p>

Cited by 34 publications

References 39 publications

<p>LncRNA PART1 Exerts Tumor-Suppressive Functions in Tongue Squamous Cell Carcinoma via miR-503-5p</p>

<p>LncRNA PART1 Exerts Tumor-Suppressive Functions in Tongue Squamous Cell Carcinoma via miR-503-5p</p>

PART1 and hsa-miR-429-Mediated SHCBP1 Expression Is an Independent Predictor of Poor Prognosis in Glioma Patients

Role of lncRNA PART1 in intervertebral disc degeneration and associated underlying mechanism

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