<p>Long Noncoding RNA LINC00839 Promotes the Malignant Progression of Osteosarcoma by Competitively Binding to MicroRNA-454-3p and Consequently Increasing c-Met Expression</p>

Abstract

This study was conducted to determine the expression and prognostic relevance of long intergenic non-protein coding RNA 839 (LINC00839) in osteosarcoma (OS) and to explore the detailed roles of LINC00839 in regulating OS cell activities and the mechanisms responsible for its cancer-promoting activity in OS. Methods: The expression of LINC00839 in OS tissues and cell lines was determined by quantitative reverse transcription-polymerase chain reaction. After LINC00839 knockdown, cell counting kit-8 assay, flow cytometric analysis, transwell migration and invasion assay, and in vivo tumor xenograft assay were used to detect its effects on cellular processes in OS. Bioinformatics analyses were conducted to predict the putative miRNAs that target LINC00839. RNA immunoprecipitation assay, luciferase reporter assay, Western blotting analysis, and rescue assays were conducted to establish a relationship among LINC00839, microRNA-454-3p (miR-454-3p), and cellular mesenchymal to epithelial transition factor (c-Met) in OS. Results: LINC00839 was upregulated in OS tissues and cell lines. OS patients characterized with high LINC00839 expression exhibited shorter overall survival than patients with low LINC00839 expression. LINC00839 knockdown caused a significant reduction in OS cell proliferation, migration, and invasion in vitro. Furthermore, LINC00839 depletion inhibited OS tumor growth in vivo and induced apoptosis. Mechanistically, LINC00839 functions as a competitive endogenous RNA in OS by sponging miR-454-3p. c-Met was confirmed as a direct target gene for miR-454-3p in OS cells and was positively regulated by LINC00839 by competitively binding to miR-454-3p. Conclusion: LINC00839 promoted the oncogenicity of OS by targeting the miR-454-3p/ c-Met axis. The LINC00839/miR-454-3p/c-Met network may represent a potential target for OS therapy.

A five metastasis-related long noncoding RNA risk signature for osteosarcoma survival prediction

“…In addition, the role of lncRNAs in osteosarcoma is currently of significant interest to many researchers. Previous studies have demonstrated the relationship between lncRNAs and tumorigenesis [ 9 , 10 ], metastasis [ 11 ], progression [ 12 ], and chemotherapeutic drug resistance [ 13 ] in osteosarcoma. Unfortunately, there is limited intervention research on the impact of potential prognostic lncRNA signatures for osteosarcoma.…”

LINC00839 Knockdown Restrains The Metastatic Behaviors of Nasopharyngeal Carcinoma By Sponging miR-454-3p

“…LINC00839 acts as an oncogenic gene and is signi cantly upregulated in human cancers including breast cancer and osteosarcoma [11,12]. In our study, we noticed that LINC00839 level was widely higher in NPC tissues from GEO dataset and cell lines than the normal tissues and NP-69.…”

“…LINC00839 has been veri ed as a cancer-promoting factor in multiple malignancies, and its dysregulation is closely correlated with tumor initiation, development and progression. In osteosarcoma, LINC00839 facilities the malignant development of cancer cell through competitively binding to miRNA-454-3p and enhancing the expression of c-Met [11]. LINC00839 is upregulated in chemo-resistant breast carcinoma cells and tissues, and high level of LINC00839 is associated with a poor prognosis [12].…”

An Overview of Long Non-Coding (lnc)RNAs in Neuroblastoma

“…Long intergenic non-protein coding RNA 839 (LINC00839) was also studied in breast cancer [100] and osteosarcoma [101]. Its upregulation correlated with bad prognosis in both cases.…”