<p>Long noncoding RNA SNHG16 silencing inhibits the aggressiveness of gastric cancer via upregulation of microRNA-628-3p and consequent decrease of NRP1</p>

Abstract

Background: MicroRNA-628-3p (miR-628) has been reported to play important roles in the progression of multiple human cancer types. Nonetheless, whether the expression profile of miR-628 is altered in gastric cancer remains unclear and whether its aberrant expression plays a crucial part in the aggressiveness of gastric cancer is yet to be determined. Therefore, in this study, we systematically investigated the involvement of miR-628 in gastric cancer progression. Materials and methods: MiR-628 expression in gastric cancer tissues and cell lines were determined via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). A CCK-8 assay, flow-cytometric analysis, Transwell assays, and a xenograft model experiment were performed to evaluate the influence of miR-628 overexpression on gastric cancer cells. Notably, the mechanisms underlying the tumor-suppressive activity of miR-628 in gastric cancer cells were explored by bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blotting. Results: MiR-628 expression was low in gastric cancer tissue samples and cell lines. The low expression of miR-628 was closely associated with the lymph node metastasis, invasive depth and TNM stage among patients with gastric cancer. Further clinical analysis indicated that patients with gastric cancer underexpressing miR-628 had a worse prognosis than did the patients with high miR-628 expression in the tumor. Overexpressed miR-628 restrained proliferation, migration, and invasion; induced apoptosis; and impaired tumor growth of gastric cancer cells. In addition, neuropilin 1 (NRP1) mRNA was validated as the direct target of miR-628 in gastric cancer. Long noncoding RNA small nucleolar RNA host gene 16 (SNHG16) was demonstrated to sponge miR-628 in gastric cancer. Moreover, miR-628 knockdown abrogated the influence of SNHG16 silencing on gastric cancer cells. Conclusion: Our findings elucidate how the SNHG16-miR-628-NRP1 pathway serves as a regulatory network playing crucial roles in gastric cancer progression, suggesting that this pathway may be a novel target of anticancer therapy.

<p>Long Noncoding RNA <em>ZFPM2-AS1</em> Enhances the Malignancy of Cervical Cancer by Functioning as a Molecular Sponge of microRNA-511-3p and Consequently Increasing FGFR2 Expression</p>

“…Luciferase reporter assay was conducted to assess the binding between ZFPM2-AS1 and miR-511-3p in cervical cancer cells. 38 FGFR2 3′ untranslated region (UTR) fragments containing either the predicted wildtype (WT) miR-511-3p-binding site or a mutant (MUT) binding site were amplified by GenePharma Technology. The amplified fragments were cloned into the pmirGLO luciferase reporter vector (Promega, Madison, WI, USA) to respectively create luciferase reporter plasmids WT-FGFR2 and MUT-FGFR2.…”

<p>Long Noncoding RNA <em>RGMB-AS1</em> Acts as a microRNA-574 Sponge Thereby Enhancing the Aggressiveness of Gastric Cancer via HDAC4 Upregulation</p>

“…[11][12][13] Notably, lncRNAs may serve as either tumor suppressors or oncogenic molecules and participate in the regulation of malignant characteristics of GC during GC progression. [14][15][16] MicroRNAs (miRNAs) comprise another group of noncoding RNA molecules, of 17-25 nucleotides in length. 17 They can directly interact with the 3′ untranslated regions (3′-UTRs) of their target mRNAs in a base-pairing manner, thereby causing mRNA degradation and/or translational suppression.…”

<p><em>SNHG16</em>: A Novel Long-Non Coding RNA in Human Cancers</p>

“…57 Another study found that miR-628 is regulated by SNHG16 and its target Neuropilin-1 (NRP1) can promote tumor metastasis. 58 SNHG16 was shown to promote cell invasion and migration in pancreatic cancer cells via mediating high mobility group box 1 (HMGB1) (a known oncogene in PC) expression through sponging miR-218-5p. 41 Wen et al found that knockdown of SNHG16 inhibits migration and invasion of TPC-1 cells via regulating miR-497.…”

“…14 Another study found that miR-628 is regulated by SNHG16 and the expression of miR-628-3p is down in GC tissues and GC cell lines, and its target NRP1 can inhibit cell apoptosis. 58…”