&lt;p&gt;&lt;em&gt;Raoultella ornithinolytica&lt;/em&gt;: Emergence and Resistance&lt;/p&gt;

Hajjar, Roy; Ambaraghassi, Georges; Sebajang, Herawaty; Schwenter, Frank; Su, S.

doi:10.2147/idr.s191387

Cited by 70 publications

(70 citation statements)

References 76 publications

(131 reference statements)

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“…In the latter case, this plasmid also harbours an additional 10 resistance genes and co-resides in isolate 6Rf with the pOXA-48-like plasmid. Thus, both Korn and Kpla isolates can harbour multidrug resistance plasmids in the environment, and improved diagnostics are required to improve the reporting of serious infections caused by these species [44,45].…”

Section: Discussionmentioning

confidence: 99%

A high prevalence of bla OXA-48 in Klebsiella (Raoultella) ornithinolytica and related species in hospital wastewater in South West England

et al. 2021

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Klebsiella species occupy a wide range of environmental and animal niches, and occasionally cause opportunistic infections that are resistant to multiple antibiotics. In particular, Klebsiella pneumoniae (Kpne) has gained notoriety as a major nosocomial pathogen, due principally to the rise in non-susceptibility to carbapenems and other beta-lactam antibiotics. Whilst it has been proposed that the urban water cycle facilitates transmission of pathogens between clinical settings and the environment, the level of risk posed by resistant Klebsiella strains in hospital wastewater remains unclear. We used whole genome sequencing (WGS) to compare Klebsiella species in contemporaneous samples of wastewater from an English hospital and influent to the associated wastewater treatment plant (WWTP). As we aimed to characterize representative samples of Klebsiella communities, we did not actively select for antibiotic resistance (other than for ampicillin), nor for specific Klebsiella species. Two species, Kpne and K. ( Raoultella ) ornithinolytica (Korn), were of equal dominance in the hospital wastewater, and four other Klebsiella species were present in low abundance in this sample. In contrast, despite being the species most closely associated with healthcare settings, Kpne was the dominant species within the WWTP influent. In total, 29 % of all isolates harboured the bla OXA-48 gene on a pOXA-48-like plasmid, and these isolates were almost exclusively recovered from the hospital wastewater. This gene was far more common in Korn (68 % of isolates) than in Kpne (3.4 % of isolates). In general plasmid-borne, but not chromosomal, resistance genes were significantly enriched in the hospital wastewater sample. These data implicate hospital wastewater as an important reservoir for antibiotic-resistant Klebsiella , and point to an unsuspected role of species within the Raoultella group in the maintenance and dissemination of plasmid-borne bla OXA-48. This article contains data hosted by Microreact.

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Section: Discussionmentioning

confidence: 99%

A high prevalence of bla OXA-48 in Klebsiella (Raoultella) ornithinolytica and related species in hospital wastewater in South West England

et al. 2021

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“…As a result, appropriate measures such as infection control and antifungal prescription may be delayed [29]. Additionally, the Autof ms1000 incorrectly identified K. oxytoca as R. ornithinolyticus, a rare pathogen in human infection [30]. With the development of identification technology, recent reports on the pathogenicity of R. ornithinolyticus have been made [30].…”

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confidence: 99%

“…Additionally, the Autof ms1000 incorrectly identified K. oxytoca as R. ornithinolyticus, a rare pathogen in human infection [30]. With the development of identification technology, recent reports on the pathogenicity of R. ornithinolyticus have been made [30]. Other species-level misidentifications, including Serratia spp., Acinetobacter spp., and Enterococcus spp., had little clinical significance, as they did not alter the interpretation of antimicrobial susceptibility [31].…”

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confidence: 99%

Comparison of Autof ms1000 and Bruker Biotyper MALDI-TOF MS Platforms for Routine Identification of Clinical Microorganisms

Park

Jang

Yoon

et al. 2021

BioMed Research International

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Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is widely used in clinical microbiology laboratories because it is cost-effective, reliable, and fast. This study is aimed at comparing the identification performance of the recently developed Autof ms1000 (Autobio, China) with that of the Bruker Biotyper (Bruker Daltonics, Germany). From January to June 2020, 205 preserved strains and 302 clinical isolates were used for comparison. Bacteria were tested with duplicates of the direct transfer method, and formic acid extraction was performed if the results were not at the species level. Fungi were tested with formic acid extraction followed by ethanol extraction methods. 16S rRNA or ITS region sequence analysis was performed on isolates that could not be identified by any of the instruments and on isolates that showed inconsistent results. The time to result of each instrument was also compared. Among preserved strains, species-level identification results were obtained in 202 (98.5%) strains by the Autof ms1000 and 200 (97.6%) strains by the Bruker Biotyper. Correct identification at the species/complex level was obtained for 200 (97.6%) strains by the Autof ms1000 and for 199 (97.1%) strains by the Bruker Biotyper. Among clinical isolates, species-level identification results were obtained in 301 (99.7%) strains and 300 (99.3%) strains by the Autof ms1000 and Bruker Biotyper, respectively. Correct identification at the species/complex level was achieved for 299 (99.0%) strains by the Autof ms1000 and for 300 (99.3%) strains by the Bruker Biotyper. The time to analyze 96 spots was approximately 14 min for the Autof ms1000 and approximately 27 min for the Bruker Biotyper. The two instruments showed comparable performance for the routine identification of clinical microorganisms. In addition, the Autof ms1000 has a short test time, making it convenient for use in clinical microbiology laboratories.

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“…Raoultella ornithinolytica is a bacterium often found in aquatic environments, soil, insects, fish, ticks, and termites [ 20 ]. This bacterium is involved in several pathologies in humans, including in the oral cavity [ 20 , 21 ].…”

Section: Resultsmentioning

confidence: 99%

Microbial biodiversity of natural toothbrushes in Mali

Sogodogo

Doumbo

Kouriba

et al. 2021

New Microbes and New Infections

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Different oral hygiene practices are used to overcome endemic diseases such as dental caries and oral infections. In Mali (Africa), natural plant-based toothbrushes are used for eliminating bacterial biofilm. The repertoire of microorganisms associated with natural toothbrushes is unknown. The aim of our study is to study microbial flora in particular the methanogenic archaea associated with natural toothbrushes recently recognized as responsible for periodontitis and peri-implantitis. We investigated the methanogens and bacteria associated with 15 different natural plant toothbrushes collected in Bamako local market (Mali). Microbiological investigations consisted in culturing the bacteria on agar plates and searching archaea using molecular techniques. No archaea were demonstrated by molecular biology but 50 bacterial species, including 33 aero-anaerobic and 17 aerobic species, were isolated from natural toothbrushes. We isolated Pseudomonas sp., Staphylococcus sp. and Klebsiella pneumoniae , which are acknowledged as opportunistic human pathogens. This study has highlighted the likely impact of the use of natural toothbrushes in the spread of potentially pathogenic bacteria in the human oral cavity.

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<p><em>Raoultella ornithinolytica</em>: Emergence and Resistance</p>

Cited by 70 publications

References 76 publications

A high prevalence of bla OXA-48 in Klebsiella (Raoultella) ornithinolytica and related species in hospital wastewater in South West England

A high prevalence of bla OXA-48 in Klebsiella (Raoultella) ornithinolytica and related species in hospital wastewater in South West England

Comparison of Autof ms1000 and Bruker Biotyper MALDI-TOF MS Platforms for Routine Identification of Clinical Microorganisms

Microbial biodiversity of natural toothbrushes in Mali

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