&lt;p&gt;&lt;strong&gt;DNA Barcoding of Endangered &lt;em&gt;Paphiopedilum &lt;/em&gt;species (Orchidaceae) of Peninsular Malaysia&lt;/strong&gt;&lt;/p&gt;

Rajaram, Micheal C.; Yong, Christina Seok Yien; Gansau, Jualang Azlan; Go, Rusea

doi:10.11646/phytotaxa.387.2.2

Cited by 22 publications

(22 citation statements)

References 22 publications

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“…The remaining 33 samples showed a similar percentage of 99,0% in comparing to Genbank (DQ464235.1 and AJ133896.1) as indicated in Table 1. Our obtained results were similar to some previous studies to use rbcL to identify the higher taxonomic levels [23][24]. As the results attained, it demonstrated that 35 samples collected from different geographical provinces were accurately the Huperzia species.…”

Section: Molecular Markers Application To Identify Huperzia Samples Bsupporting

confidence: 90%

Evaluation of Genetic Diversity and Identification of <i>Huperzia</i> Species Collected in Some Different Areas in Vietnam by Molecular Markers

Hà

Khanh

Trung

2020

ILNS

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The objective of this study was to evaluate genetic diversity of 35 Huperzia samples collected from the different ecological areas in Vietnam by using rbcL markers. The results revealed that the samples were genetically diverse in high genetic similarity which ranged from 98.0 to 99.0%, respectively. Based on the Genbank data in comparison, it was ascertained that only one sample (BK3) is a member of H.phlegmarioides group, and others belonged H. squarrosa group as divided into two main groups by phylogenetic tree analyses. It suggests that some Huperzia samples may plausibly descend from the same origin, evolution and arising relations. This study has provided useful information for further identifying the high-quality genetic plant sources for propagation, development and conservation of the high-valued medicinal materials of Huperzia plants in this country.

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Section: Molecular Markers Application To Identify Huperzia Samples Bsupporting

confidence: 90%

Evaluation of Genetic Diversity and Identification of <i>Huperzia</i> Species Collected in Some Different Areas in Vietnam by Molecular Markers

Hà

Khanh

Trung

2020

ILNS

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“…Although matK was proposed as the best barcode, with 100% resolution in two previous Paphiopedilum studies [28,41], our study agreed with Parveen et al (2017), who found that denser sampling decreased the resolution of matK [42]. However, the combination of matK with another locus was recommended as well.…”

Section: Genetic Diversity and Identification Of The Paphiopedilum Posupporting

confidence: 78%

“…Although low-copy nuclear genes were not included in this study, the authors recommended the use of these regions in the DNA barcoding of this genus for more precise identification [45]. Recently, Rajaram et al (2019) worked on four Paphiopedilum species of Peninsular Malaysia for discriminatory power using rbcL, matK, ITS, and trnH-psbA loci. In that study, matK was also shown to have the highest capability of resolution (100%) [28], as described by Parveen et al (2012) [41].…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Identification of Vietnamese Paphiopedilum Species Using DNA Sequences

Nguyen

et al. 2019

Biology

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Paphiopedilum is among the most popular ornamental orchid genera due to its unique slipper flowers and attractive leaf coloration. Most of the Paphiopedilum species are in critical danger due to over-exploitation. They were listed in Appendix I of the Convention on International Trade in Endangered Species of Wild Fauna and Flora, which prevents their being traded across borders. While most Paphiopedilum species are distinctive, owing to their respective flowers, their vegetative features are more similar and undistinguished. Hence, the conservation of these species is challenging, as most traded specimins are immature and non-flowered. An urgent need exists for effective identification methods to prevent further illegal trading of Paphiopedilum species. DNA barcoding is a rapid and sensitive method for species identification, at any developmental stage, using short DNA sequences. In this study, eight loci, i.e., ITS, LEAFY, ACO, matK, trnL, rpoB, rpoC1, and trnH-psbA, were screened for potential barcode sequences on the Vietnamese Paphiopedilum species. In total, 17 out of 22 Paphiopedilum species were well identified. The studied DNA sequences were deposited to GenBank, in which Paphiopedilum dalatense accessions were introduced for the first time. ACO, LEAFY, and trnH-psbA were limited in amplification rate for Paphiopedilum. ITS was the best single barcode. Single ITS could be used along with nucleotide polymorphism characteristics for species discrimination. The combination of ITS + matK was the most efficient identification barcode for Vietnamese Paphiopedilum species. This barcode also succeeded in recognizing misidentified or wrongly-named traded samples. Different bioinformatics programs and algorithms for establishing phylogenetic trees were also compared in the study to propose quick, simple, and effective tools for practical use. It was proved that both the Bayesian Inference method in the MRBAYES program and the neighbor-joining method in the MEGA software met the criteria. Our study provides a barcoding database of Vietnamese Paphiopedilum which may significantly contribute to the control and conservation of these valuable species.

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“…The matK sequences discriminate the closely related P. barbatum and P. callosum var. sublaeve, therefore supporting the species circumscription by Cribb (1998) (Rajaram et al 2019). Nevertheless, slipper orchids are infamously variable, and unusual plants may sometimes be natural hybrids, especially when the putative parents grow sympatrically (Averyanov et al 2007, Leong 2014, van der Ent et al 2015.…”

Section: Introduction Paphiopedilum Originates From Thementioning

confidence: 77%

“…P.J.Cribb belong to subgenus Paphiopedilum (Seidenfeiden & Wood 1992, Cribb 1998, Leong 2014. A work on DNA Barcoding of Endangered Paphiopedilum species of Peninsular Malaysia using four DNA barcode loci and their combinations (rbcL, matK, ITS, trnH-psbA) published by Rajaram et al (2019) clusters each species as a monophyletic clade. The matK sequences discriminate the closely related P. barbatum and P. callosum var.…”

Section: Introduction Paphiopedilum Originates From Thementioning

confidence: 99%

Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia

et al. 2021

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Abstract. The floral micromorphology of critically endangered Paphiopedilum Pfitzer [P. barbatum (Lindl.) Pfitzer, P. callosum var. sublaeve (Rchb.f.) P.J.Cribb and P. niveum (Rchb.f.) Stein] were analyzed concerning either infrageneric taxonomy or physioecological demands. The first two species are phylogenetically close and superficially identical but occur with distinct phytogeographical distributions in the region. The third species is a phylogenetically distant congener that inhabits limestone areas in the northern part of Peninsular Malaysia. Using scanning electron microscopy (SEM), we investigated the surface of the dorsal sepal, synsepal, lateral petals, pouch or labellum, and staminode. Amongst the investigated features were epicuticular waxes, epicuticular ornamentation, trichome distribution and type, pustular glands, and papillae. Our study supports the distinction of P. barbatum from P. callosum var. sublaeve, which belong to subgenus Paphiopedilum, and from P. niveum, a species belonging to subgenus Brachypetalum, a separated monophyletic clade. Comparatively, P. barbatum has Type III non-glandular trichomes on the margin of its lateral petals, which are absent in P. callosum var. sublaeve. Paphiopedilum callosum var. sublaeve and P. niveum are distinguishable from P. barbatum by a confined distribution of papillae. The epicuticular ornamentation and distribution of trichomes on staminode discriminate between P. barbatum and P. callosum var. sublaeve and differentiates them from P. niveum. Compared to P. barbatum and P. niveum, stomata in P. callosum var. sublaeve were superficial with prominently raised guard cells. From the physioecological view, the absence of glandular trichomes, and the low occurrence of papillae and stomata on the floral parts explain the unscented flowers of P. barbatum and P. callosum var. sublaeve. A combination of the features examined is taxonomically valuable for delimitation of the species at the infrageneric level, although the diagnostic characters are far inadequate for a generic taxonomic revision. A study with a more extensive sampling from the three subgenera of Paphiopedilum, including subgenus Parvisepalum, is anticipated to elucidate the level of variation of the analyzed microcharacters. Key Words: physioecological importance, Peninsular Malaysia, subgenus Brachypetalum, subgenus Paphiopedilum, scanning electron microscopy, taxonomic delimitation

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<p><strong>DNA Barcoding of Endangered <em>Paphiopedilum </em>species (Orchidaceae) of Peninsular Malaysia</strong></p>

Cited by 22 publications

References 22 publications

Evaluation of Genetic Diversity and Identification of <i>Huperzia</i> Species Collected in Some Different Areas in Vietnam by Molecular Markers

Evaluation of Genetic Diversity and Identification of <i>Huperzia</i> Species Collected in Some Different Areas in Vietnam by Molecular Markers

Genetic Diversity and Identification of Vietnamese Paphiopedilum Species Using DNA Sequences

Comparative floral surface micromorphology helps to discriminate between species of Paphiopedilum (Orchidaceae: Cypripedioideae) from Peninsular Malaysia

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