&lt;p&gt;&lt;strong&gt;&lt;em&gt;Termitomyces acriumbonatus&lt;/em&gt;&lt;/strong&gt;&lt;strong&gt; &lt;em&gt;sp. nov.&lt;/em&gt; (Lyophyllaceae, Agaricales) from Pakistan&lt;/strong&gt;&lt;/p&gt;

Usman, Muhammad; Khalid, Abdul Nasir

doi:10.11646/phytotaxa.477.2.6

Cited by 15 publications

(9 citation statements)

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“…Primers used during amplifications were ITS1F and ITS4 for the ITS region, with LR0R and LR5 for the nuLSU region (White et al 1990; Gardes & Bruns 1993). Polymerase chain reaction (PCR) conditions adapted from those of Gardes & Bruns (1993) were followed according to Usman & Khalid (2020). The PCR amplicons were purified using a QIAquick PCR Purification Kit (Qiagen, Valencia, CA, USA) and then sent for sequencing at TsingKe, China, using the aforementioned primers.…”

Section: Methodsmentioning

confidence: 99%

A new species of the genusAnamylopsora(Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan

et al. 2023

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A novel lichen species occurring on rocks was collected from three different localities within Deosai National Park, Gilgit-Baltistan, Pakistan. Phylogenetic analyses of the nrDNA ITS and nuLSU regions revealed that it clustered within the genus Anamylopsora. Further chemical and morpho-anatomical analyses confirmed its uniqueness, and it is described here as a new species under the name A. pakistanica. The distinguishing characters are: an irregularly squamulose appressed thallus on rocks without rhizines; an epinecral layer up to 25 μm thick; ascospores that are hyaline, simple, thick-walled with a smooth surface; septate paraphyses with a pigmented apical cell in a gel-like matrix; globose to subglobose pycnidia with hyaline and bacilliform pycnidiospores. In particular, the species is distinguished from other members of the genus by morpho-anatomical features including the coloration of the thalli, the presence of a thick lower cortex (up to 100 μm), and the presence of simple, thick-walled ascospores. Specimens were found at altitudes up to 4587 m, the highest elevation yet reported for Anamylopsora. A key and comparison to all existing species of the genus Anamylopsora is also given.

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Section: Methodsmentioning

confidence: 99%

A new species of the genusAnamylopsora(Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan

et al. 2023

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“…The modified CTAB method given by Zhao et al (2011) was used for the extraction of DNA from the dried specimens (Figure 2). For PCR amplifications the conditions were followed as mentioned in Usman and Khalid (2020) and the primer pair ITS1F and ITS4 for the ITS region and LR0R and LR5 for the nrLSU were selected (Gardes & Bruns, 1993; Vilgalys & Hester, 1990; White et al, 1990).…”

Section: Methodsmentioning

confidence: 99%

Identification and taxonomic position of a new Pseudoomphalina species from Pakistan based on light, scanning electron microscopy, and molecular analysis

Rani,

Hina,

Iqbal

et al. 2023

Microscopy Res & Technique

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Khanspur, a small town located near Ayubia National Park, is a famous mountainous resort lying in Galyat area of Khyber Pakhtunkhwa. It is included in one of the richest biodiversity hotspots in the country. Despite of extensive previous studies, many new species are awaiting to be documented, including macrofungi. In this study, a new macrofungus Pseudoomphalina khanspurensis is analyzed by both light and scanning electron microscopy as well as DNA sequences based on nrITS and nrLSU regions. The sister species P. cokeri differs by its red to purple, dark to reddish brown, broadly convex to applanate pileus, purple blue to brownish stipe, and numerous cylindrical to lageniform cheilocystidia.Highlights This is the first report of genus Pseudoomphalina from Pakistan and first study based on Scanning electron microscopy. These species have been described with detailed micro‐morphological and molecular makers (nrITS and nrLSU). General distribution, ecology, diagnostic features and comparisons with alies have been provided. Graphical representation of DNA extraction and geographical locations of sampling sites are also illustrated. Softwares such as CIPRES Science Gateway Portal, MUSCLE, BioEdit, FigTree, Adobe illustrator and Adobe Photoshop are used in the current study. No member of this genus is already described from Pakistan.

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“…The primer pair ITS1F (Gardes & Bruns, 1993) and ITS4 (White et al, 1990) was used to amplify the internal transcribed spacer (ITS) region. Polymerase chain reaction (PCR) conditions adapted from those of Gardes and Bruns (1993) were followed according to Usman and Khalid (2020). The PCR amplicons were purified using a QIAquick PCR Purification Kit (Qiagen, Valencia, CA, USA) PCR products were sent to China, where both strands were sequenced.…”

Section: Methodsmentioning

confidence: 99%

Section: Dna Extraction Pcr Amplification and Sequencingmentioning

confidence: 99%

New species and new records of genus Buellia (lichenized Ascomycota, Caliciaceae) from Pakistan using electron microscopy and DNA barcoding techniques

Afshan,

Fayyaz,

Iftikhar

et al. 2023

Microscopy Res & Technique

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Three new species Buellia darelensis, B. densitheca, B. kashmirensis and two new records B. elegans and B. taishanensis are added to the lichen biota of Pakistan. Buellia darelensis and B. kashmirensis share the same habitats found on the rock while B. densitheca colonized on bark of Pinus hardwoods in the Himalayan forest, Pakistan. Morphological, chemical, and phylogenetic analyses were carried out to elucidate the placement of these species and to support the delimitation of the new taxa. Detailed descriptions and figures for the species are given, and a key to all known buellioid species from Pakistan is provided.Research Highlights During recent explorations of lichens from different regions of Pakistan, we observed specimens that could not be readily assigned to any known species. A phylogenetic analysis of the internal transcribed spacer nrDNA region confirms their position within the genus Buellia, and morphological data showed distinctiveness of three species from other known species of the genus. We therefore describe these specimens as new species to science, and two species are as new records for the country. Pakistan exhibits a large altitudinal variation, with climatic conditions and a diverse vegetation that supports a diverse and conspicuous lichen biota. The nature reserves have abundant biological resources, and it is expected that more new species of lichen may be discovered in the future.

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<p><strong><em>Termitomyces acriumbonatus</em></strong><strong> <em>sp. nov.</em> (Lyophyllaceae, Agaricales) from Pakistan</strong></p>

Cited by 15 publications

References 1 publication

A new species of the genusAnamylopsora(Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan

A new species of the genusAnamylopsora(Baeomycetaceae; Ascomycota) from Deosai National Park, Gilgit-Baltistan, Pakistan

Identification and taxonomic position of a new Pseudoomphalina species from Pakistan based on light, scanning electron microscopy, and molecular analysis

New species and new records of genus Buellia (lichenized Ascomycota, Caliciaceae) from Pakistan using electron microscopy and DNA barcoding techniques

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