&lt;p&gt;MicroRNA-505 suppresses gastric cancer cell proliferation and invasion by directly targeting Polo-like kinase-1&lt;/p&gt;

Dang, Sheng-Chun; Wang, Fei; Qian, Xiao-Bao; Malik, Abdul; Naseer, Qais-Ahmad; Jin, Wei; Hu, Rong; Gu, Qian; Gu, Min

doi:10.2147/ott.s189521

Cited by 12 publications

(8 citation statements)

References 24 publications

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“…11 Abnormal expression of miR-505 has been observed in various cancers. Dang et al 12 found that miR-505 had some connections with GC cell proliferation and invasion. Zhong et al 13 pointed out that miR-505 functioned as a tumor suppressor in RCC by downregulating high mobility group box-1 protein (HMGB1).…”

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confidence: 99%

Retracted: The circular RNA‐NRIP1 plays oncogenic roles by targeting microRNA‐505 in the renal carcinoma cell lines

Dong

Liu

Wang

et al. 2019

J of Cellular Biochemistry

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We explored the roles and regulatory mechanisms of the circular RNA (circRNA) nuclear receptor-interacting protein 1 (NRIP1; circNRIP1) in ACHN and CAKI-1 cells. ACHN and CAKI-1 cells were transfected with smallinterfering-circNRIP1 (si-circNRIP1) and microRNA-505 (miR-505) inhibitor or the corresponding controls. Cell viability was detected with the Cell Counting Kit-8. The protein expression levels of Bcl-2, Bax, cleaved-caspase-3, matrix metalloproteinase (MMP)-2, MMP-9, adenosine 5′-monophosphate (AMP)activated protein kinase (AMPK), protein kinase B (AKT), phosphatidylinositol 3-kinase (PI3K), and mammalian target of rapamycin (mTOR) were individually determined via Western blot. Quantitative reverse transcription polymerase chain reaction was used to examine the expressions of circNRIP1 and miR-505 both in tumor cells and tissues. The apoptotic rate, the colony numbers, and the migration rate were separately determined by the Annexin V-fluorescein isothiocyanate/propidium iodide and flow cytometer, colony formation assay, and migration assay. We found that circNRIP1 was overexpressed in tumor tissue but miR-505 was overproduced. Silencing circZNF292 induced inhibition of cell viability, colony formation, and migration, as well as the activity of AMPK and PI3K/AKT/mTOR cascades but enhancement of apoptosis. si-circNRIP1 stimulated the upregulation of miR-505, whose silence abolished the effects of si-circNRIP1 on these elements mentioned above. In conclusion, the circNRIP1 played oncogenic roles in the ACHN and the CAKI-1 cell lines by targeting miR-505 via stimulating AMPK and PI3K/AKT/mTOR cascades.

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confidence: 99%

Retracted: The circular RNA‐NRIP1 plays oncogenic roles by targeting microRNA‐505 in the renal carcinoma cell lines

Dong

Liu

Wang

et al. 2019

J of Cellular Biochemistry

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“…EMT is a great contributor to the CC cell chemotherapy and radiotherapy resistance; thus, inhibiting EMT improves the survival rate of CC patients by sensitizing the CC cells to radiotherapy and drugs [ 32 ]. The miR-505 mimic elevated N-cadherin expression but decreased E-cadherin in gastric cancer cells [ 38 ]. miR-505-5p was correlated with metastasis, and the overexpression of miR-505-5p inhibited metastasis and EMT in CC cells [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2

Zhang

et al. 2020

Analytical Cellular Pathology

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The application of ultrasound and microbubbles (USMB-) mediated microRNA (miR) is a promising approach of gene delivery for cancer treatment. We aimed to discuss the effects of USMB-miR-505 on cervical cancer (CC) development. miR-505 mediated by USMB was prepared. The effect of miR-505 on its transfection efficiency and the effect of miR-505 on HeLa cell proliferation, cell cycle, apoptosis, migration, and invasion were studied. The target gene of miR-505 was predicted, and its expression in CC was detected. The effect of the target gene on HeLa cells was further verified. USMB-miR-505 showed a higher transfection efficiency than miR-505 alone. The inhibitory effect of miR-505 mediated by USMB on HeLa cells was better than miR-505. miR-505 targeted AKT2, which was upregulated in CC. Overexpression of AKT2 reversed the inhibitory effect of USMB-miR-505 on HeLa cell malignant behaviors. Overall, we highlighted that USMB-miR-505 inhibited HeLa cell malignant behaviors by targeting AKT2.

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“…EMT contributes to chemotherapy and radiotherapy resistance of CC cells, thus inhibiting EMT sensitizes CC cells sensitive to radiotherapy and drugs, and improves the survival rate of CC patients [32]. miR-505 mimic elevated Ncadherin expression but decreased E-cadherin in gastric cancer cells [36]. miR-505-5p was correlated with metastasis, and overexpression of miR-505-5p inhibited metastasis and EMT in CC cells [37].…”

Section: Discussionmentioning

confidence: 99%

Ultrasound Microbubbles-mediated Microrna-505 Regulates Cervical Cancer Cell Growth via AKT2

Zhang

et al. 2020

Preprint

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Background: The gene-loaded microbubbles (MBs) combined with ultrasound resulting in increased delivery efficiency, may be a novel method of gene delivery. We explored the effects of ultrasound and microbubbles (USMB)-mediated microRNA (miR)-505 on cervical cancer (CC) development.Methods: miR-505 mediated by USMB was prepared. The effect of miR-505 on its transfection efficiency was studied by RT-qPCR. The effect of miR-505 on HeLa cell proliferation was evaluated by MTT and colony formation assays. Flow cytometry was used to study cell cycle changes, Hoechst was utilized to detect apoptosis. Through the wound healing and Transwell assay, the migration and invasion ability of HeLa cells were measured. The target gene of miR-505 was predicted, and its expression in CC was detected. The target relationship and the effect of the target gene on HeLa cells were further verified.Results: USMB-miR-505 showed higher transfection efficiency than miR-505 alone. miR-505 inhibited HeLa cell malignant episodes, which were reinforced by USMB treatment. miR-505 targeted AKT2. AKT2 was highly expressed in CC, and overexpression of AKT2 significantly reversed the inhibitory effect of miR-505 mediated by USMB on HeLa cell malignant biological behaviors.Conclusion: USMB-miR-505 inhibited HeLa cell malignant biological behaviors by targeting AKT2.

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<p>MicroRNA-505 suppresses gastric cancer cell proliferation and invasion by directly targeting Polo-like kinase-1</p>

Cited by 12 publications

References 24 publications

Retracted: The circular RNA‐NRIP1 plays oncogenic roles by targeting microRNA‐505 in the renal carcinoma cell lines

Retracted: The circular RNA‐NRIP1 plays oncogenic roles by targeting microRNA‐505 in the renal carcinoma cell lines

Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2

Ultrasound Microbubbles-mediated Microrna-505 Regulates Cervical Cancer Cell Growth via AKT2

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