<p>miR-101 regulates cell proliferation and apoptosis by targeting KDM1A in diffuse large B cell lymphoma</p>

Abstract

Background miR-101 is reported to be associated with cell proliferation and apoptosis. However, it is unknown whether miR-101 expression affects cell proliferation and apoptosis in diffuse large B cell lymphoma (DLBCL). The aim of the present study was to investigate the expression of miR-101 and its effect on cell proliferation and apoptosis in DLBCL. Methods miR-101 expression was detected in 30 cases of patients with DLBCL and normal lymph node by qRT-PCR. Then, miR-101 expression was up-regulated and down-regulated in Originated Cell Line-Large Lymphoma 8 (OCL-LY8) cell line, respectively. MTT and flow cytometry assay were used to evaluate the effect of miR-101 on cell proliferation and apoptosis, respectively. As KDM1A was confirmed to be as a specific target of miR-101 by TargetScanHuman, the relationship between MiR-101 and KDM1A was further investigated. Results miR-101 expression in patients with DLBCL was significantly reduced compared those in normal lymph node (P<0.05). miR-101 expression was significantly associated with tumor size, clinical stage and International Prognostic Index (IPI) scores (P<0.05). In OCL-LY8 cell line, miR-101 down-regulation significantly promoted cell proliferation and suppressed cell apoptosis. Meanwhile, miR-101 up-regulation reversed this effect. In addition, miR-101 negatively regulated the expression of KDM1A. KDM1A down-regulation was oberved in normal tissues compared with those in DLBCL tissues, which inhibited cell proliferation and promoted cell apoptosis. Conclusion These data indicate that miR-101 regulates cell proliferation and apoptosis by targeting KDM1A, which provides a potential therapeutic for DLBCL patients.

miR-101, miR-548b, miR-554, and miR-1202 are reliable prognosis predictors of the miRNAs associated with cancer immunity in primary central nervous system lymphoma

“…Further, we limited the miRNA predictors into a low-dimensional formula by harboring only four miR-NAs that included miR-101, miR-548b, miR-554, and miR-1202. miR-101 is associated with proliferation and apoptosis in DLBCL [34]. While, in glioblastoma, the miR-101 functions as a tumor suppressor targeting Krüppel-like factor (KLF)6 [35].…”

Dysregulation of miRNAs in DLBCL: Causative Factor for Pathogenesis, Diagnosis and Prognosis

“…miR-101 acts as tumor suppressor by targeting the 3 UTR of KDM1A, thereby reducing tumor growth and progression. In addition, the KDM1A mediated suppression by miR-101 suggests its impact in regulating apoptosis and cell proliferation through MAPK/Erk signaling pathway [112].…”

<p>Non-Coding RNAs in Diffuse Large B-Cell Lymphoma</p>

“… 40 Moreover, miR-101 downregulation significantly promoted cell proliferation and suppressed cell apoptosis by targeting lysine-specific histone demethylase 1A ( KDM1A ). 41 The overexpression of miR-214 significantly attenuates the malignant phenotype of DLBCL OCI-Ly3 cells in vitro and restricts tumor growth in a xenograft mouse model by targeting programmed death-ligand 1 ( PD-L1 ). 21 At the post-transcriptional level, miR-224 was determined to regulate CD59 expression and influence cell proliferation, invasion, and apoptosis.…”