<p>miR-29a inhibits proliferation, invasion, and migration of papillary thyroid cancer by targeting DPP4</p>

Abstract

Purpose: The purpose of this study was to investigate the effects of miR-29a on papillary thyroid cancer (PTC) and its underlying mechanisms. Methods: Primary tumor tissues and adjacent tissues of 69 patients with PTC were obtained. Human thyroid cell line Nthy-ori3-1 and PTC cell lines K1, BCPAP, TPC-1 were cultured. K1 cells were transfected and divided into following groups: blank group (without any treatment), miR-29a mimics group, control mimics group, miR-29a inhibitor group, control inhibitor group, DPP4 siRNA group, control siRNA group and miR-29a inhibitor + DPP4 siRNA group. qRT-PCR and Western blot were used to detect miR-29a and DPP4 expression. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and transwell assay were performed to detect cells proliferation, migration, and invasion. A nude mice xenograft experiment was performed. Results: miR-29a was significantly downregulated in PTC tissues, K1 and TPC-1 cells ( P <0.01). DPP4 was significantly upregulated in the miR-29a inhibitor group and significantly downregulated in the miR-29a mimics group ( P <0.01). DPP4 was the target gene of miR-29a. miR-29a significantly inhibited K1 cell proliferation, invasion, migration and PTC growth in nude mice by targeting DPP4 ( P <0.01). Conclusion: miR-29a inhibits proliferation, migration, and invasion of PTC by targeting DPP4, which might provide a new target for clinical treatment of PTC.

Roles of the SNHG7/microRNA‑9‑5p/DPP4 ceRNA network in the growth and 131I resistance of thyroid carcinoma cells through PI3K/Akt activation

“…Following the result that SNHG7 could target miR-9-5p, we further predicted the target genes of miR-9-5p through starBase and TargetScan. Among the target genes, DPP4 has been documented to increase the growth and metastasis of PTC cells ( 15 ). starBase prediction revealed that miR-9-5p could bind to a specific site in the 3′-UTR of DPP4 (P<0.05) ( Fig.…”

“…DPP4 serves as a tumor biomarker since it is inversely associated with survival in most cancer types ( 39 ). Furthermore, it has been documented that upregulation of DPP4 is involved in the growth and development of PTC cells ( 15 ). Herein, it was demonstrated that SNHG7 promoted the growth and 131 I resistance of PTC cells through the SNHG7/miR-9-5p/DPP4 ceRNA network.…”

MicroRNA-29a Inhibits the Occurrence of Endometrial Carcinoma by Regulating mTOR Signal Pathway Through STAT3

“…For example, miR-29a is a tumor suppressor miRNA in gliomas, which inhibits tumor stem cells and tumor growth by regulating PDGF pathway [19]. miR-29a inhibits the proliferation, migration and invasion of thyroid cancer by targeting DPP4, which may provide a new target for clinical treatment of thyroid cancer [20]. MIR-29 family regulates MTX drug resistance and apoptosis by regulating COL3A1 or MCL1.…”

Decreased serum exosomal miR‐29a expression and its clinical significance in papillary thyroid carcinoma

“…Wang and colleagues showed that miR‐29a was dramatically reduced in PTC tissues and cells. Restoration of miR‐29a significantly attenuated cancer cell proliferation, invasion, migration in vitro, and tumor growth in vivo by regulating DPP4 13 . Likewise, a reduction in miR‐29a expression was observed in PTC tissues and its downregulation was strongly associated with worse clinical features.…”

“…Restoration of miR-29a significantly attenuated cancer cell proliferation, invasion, migration in vitro, and tumor growth in vivo by regulating DPP4. 13 Likewise, a reduction in miR-29a expression was observed in PTC tissues and its downregulation was strongly associated with worse clinical features. miR-29a upregulation or AKT3 inhibition greatly restrained carcinogenesis and metastasis of PTC in vitro and in vivo.…”