<p>miR-526b-3p serves as a prognostic factor and regulates the proliferation, invasion, and migration of glioma through targeting WEE1</p>

Abstract

Background: MicroRNAs play important roles in cancer progression including glioma. In this study, we aimed to explore the expression pattern, prognostic potential, and functional role of miR-526b-3p in human glioma. Materials and methods: The expression of miR-526b-3p in glioma tissues and the adjacent non-tumor tissues was determined by quantitative RT-PCR. The chi-square test was performed to evaluate the statistical associations between miR-526b-3p level and patient characteristics. The prognostic value of miR-526b-3p was analyzed by Kaplan–Meier and Cox regression analyses. The function of miR-526b-3p was analyzed by MTT, colony formation assay, transwell assay, and flow cytometry analysis in vitro. The binding between miR-526b-3p and predicted target WEE1 was verified using dual luciferase assay and Western blot analysis. Results: We found that miR-526b-3p expression was significantly downregulated in both glioma tissues and cell lines. Downregulation of miR-526b-3p was significantly associated with advanced WHO grade, lower KPS score, and inferior patient outcomes. Functional investigation indicated that overexpression of miR-526b-3p suppressed cell proliferation, migration, and invasion, and promoted apoptosis in glioma cell lines. Mechanically, WEE1 was identified as direct targets of miR-526b-3p and overexpression of WEE1 significantly suppressed the levels of WEE1. Moreover, re-introduction of WEE1 abrogates the suppression of motility and invasiveness induced by miR-526b-3p in glioma cells. Conclusion: These findings indicate that miR-526b-3p may target WEE1 and inhibit glioma tumorigenesis and progression.

<p>Long Noncoding RNA HEIH Promotes Proliferation, Migration and Invasion of Retinoblastoma Cells Through miR-194-5p/WEE1 Axis</p>

“…29 Recently, several studies demonstrated that WEE1 were implicated in tumor growth and metastasis in laryngeal carcinoma, glioma, non-small cell lung cancer, bladder cancer and hepatocellular carcinoma. [30][31][32][33][34] In this study, we observed that miR-194-5p could directly target WEE1 3ʹUTR and regulating its expression, which was consistent with Li's report of miR-194 targeting WEE1 in laryngeal squamous cell carcinoma. 35 Additionally, HEIH knockdown significantly reduced WEE1 expression via miR-194-5p.…”

Identification and validation of a three-gene signature as a candidate prognostic biomarker for lower grade glioma

“…Those three genes included in the model were WEE1, SEMA4G, CRTAC1. It has been reported that WEE1 is closely related to the growth, invasion and migration of glioma (Wu et al, 2019). Currently, there is no study revealing the role of SEMA4G and CRTAC1 in gliomas.…”

Quercetin radiosensitizes non‐small cell lung cancer cells through the regulation of miR‐16‐5p/WEE1 axis

“…WEE1 prolongs the G2 phase via controlling the activity of CDK1, thus allowing more time for DNA repair . WEE1 is up‐regulated in diverse tumors to exert carcinogenic effects, such as in laryngeal squamous cell carcinoma, neuroblastoma, and glioma, implying that WEE1 may be a promising target for cancer treatment . Furthermore, it has been demonstrated that WEE1 expression is modulated by multiple miRNAs.…”

“…40,41 WEE1 is up-regulated in diverse tumors to exert carcinogenic effects, such as in laryngeal squamous cell carcinoma, neuroblastoma, and glioma, implying that WEE1 may be a promising target for cancer treatment. [42][43][44] Furthermore, it has been demonstrated that WEE1 expression is modulated by multiple miRNAs. For instance, miR-503 enhances the radiosensitivity of laryngeal cancer cells via targeting WEE1.…”