<p>MIR22HG As A Tumor Suppressive lncRNA In HCC: A Comprehensive Analysis Integrating RT-qPCR, mRNA-Seq, And Microarrays</p>

Abstract

Introduction: MIR22HG has a reported involvement in the tumorigenesis of a variety of cancers, including hepatocellular carcinoma (HCC). However, the exact molecular mechanism of MIR22HG in HCC has not been clarified. Methods: In the present study, we integrated data from in-house RT-qPCR, RNA-sequencing, microarray, and literature studies to conduct a comprehensive evaluation of the clinicopathological and prognostic significance of MIR22HG in an extremely large group of HCC samples. We also explored the potential mechanism of MIR22HG in HCC by analyzing the alteration profiles of MIR22HG in HCC to predict transcription factors (TFs) that may interact with MIR22HG and to annotate the biological functions of genes co-expressed with MIR22HG. MIR22HG expression was also compared in HCC nude mice xenografts before and after a treatment with nitidine chloride. Results: We found that MIR22HG was downregulated in HCC and that this downregulation correlated with the malignant phenotype of HCC. Comprehensive analysis of the prognostic impact of MIR22HG in HCC revealed a beneficial effect of MIR22HG on the survival outcome of HCC patients. Seven cases of MIR22HG deep deletion occurred in 360 of the cancer genome atlas (TCGA) provisional HCC samples. A total of 22 MIR22HG-TF-mRNA triplets in HCC were predicted by the lncRNAmap. Co-expressed genes of MIR22HG, identified by weighted correlation network analysis (WGCNA), mainly participated in the pathways involving osteoclast differentiation, chemokine signaling pathways, and hematopoietic cell lineage. In vivo experiments demonstrated that nitidine chloride could stimulate MIR22HG expression in HCC xenografts. Conclusion: In summary, MIR22HG may play a tumor-suppressive role in HCC by coordinating with predicted TFs and co-expressed genes, such as NLRP3, CSF1R, SIGLEC10, and ZEB2, or by being controlled by nitidine chloride.

The clinical value and potential molecular mechanism of the downregulation of MAOA in hepatocellular carcinoma tissues

“…As demonstrated previously, a decreased MAOA was detected in HCC patients and correlated with poor prognosis. Our previous studies have confirmed the anti‐HCC effect of NC 34,35,37 . In this study, in vivo experiment showed that the tumor volumes were reduced and the MAOA expression level was significantly elevated in the NC‐treated group compared with the control group.…”

“…Our team has been studying the biological function NC has on HCC for a period. Previous in vitro and in vivo studies have verified that TCM NC exhibits a strong anti‐HCC capability by mediating complicated mechanisms, namely TF‐miRNA‐mRNA axis, circRNA‐miRNA‐mRNA network, andJAK1/STAT3 cascade signaling inhibition 34‐38 . Given the role that MAOA plays in HCC, we supposed that NC could exert its anti‐tumor effect partially by influencing the expression of MAOA .…”

The Ubiquitin E3 Ligase TRIM21 Promotes Hepatocarcinogenesis by Suppressing the p62-Keap1-Nrf2 Antioxidant Pathway

“…We first examined the expression of TRIM21 in human HCC using publicly available datasets. In the Gene Expression Omnibus Series, 19 6 of 18 studies showed elevated TRIM21 expression in HCC comparing with normal liver tissues ( Figure 1 A ). The Cancer Genome Atlas (TCGA) also showed that TRIM21 transcript level is elevated in tumor tissues and positively correlates with disease progression ( Figure 1 B ), and that the expression level of TRIM21 correlates with a worse overall survival although not with disease-free survival ( Figure 1 C ).…”

Long non-coding RNA MIR22HG promotes osteogenic differentiation of bone marrow mesenchymal stem cells via PTEN/ AKT pathway

“…LncRNA MIR22HG, which is located on chromosome 17p13.3, has been discovered to play roles in a variety of cancers. Many previous studies revealed that MIR22HG exhibited tumor-suppressive role in several types of cancer including lung cancer, hepatocellular carcinoma, endometrial cancer, gastric cancer and cholangiocarcinoma, and its low expression was associated with poor prognosis [12][13][14][15][16][17] . Conversely, some studies showed that MIR22HG played an oncogenic function in several cancer types such as glioblastoma, and MIR22HG knockdown inhibits the invasion and proliferation of cancer cells 18 .…”