&lt;p&gt;Network pharmacology study on the active components of &lt;em&gt;Pterocypsela elata&lt;/em&gt; and the mechanism of their effect against cerebral ischemia&lt;/p&gt;

Niu, Bingxuan; Zhang, Hui; Li, Chunyan; Yan, Fei; Song, Yu; Hai, Guangfan; Jiao, Yunjuan; Feng, Yan

doi:10.2147/dddt.s207955

Cited by 21 publications

(14 citation statements)

References 18 publications

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“…Currently, network pharmacology has achieved satisfactory results in the pharmacological mechanism of TCM [ 9 ]. Multiple studies have proved that network pharmacological analysis can be used as a technique of predicting active components and key targets of TCM for tumor treatment [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Study on the molecular mechanism of nightshade in the treatment of colon cancer

Song

Cheng

et al. 2022

Bioengineered

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The present study attempts to explore the effective components, action targets, and potential mechanism of nightshade for colon cancer treatment. The relationship network diagram of ‘traditional Chinese medicine – component – target – disease’ was firstly constructed by employing network pharmacology. Experiments were conducted in vivo and in vitro to verify the influence of quercetin, the core effective component of nightshade, on colon cancer. Meanwhile, the regulatory effects of quercetin on core targets and main signaling pathways were determined. Based on the network diagram of ‘traditional Chinese medicine – component – target – disease’ and KEGG analysis, quercetin might exhibit certain effects on colon cancer treatment by regulating the biological behavior of core targets related to cell apoptosis in tumors including PIK3R1, PIK3CA, Akt1, and Akt2. Furthermore, quercetin has been demonstrated in vitro experiments to suppress the proliferation and migration of colon cancer cells whereas promote their apoptosis in a dose-dependent fashion. In vivo experiments indicate that quercetin had an antitumor effect on human colon cancer SW480 cells in nude mice bearing tumors. Furthermore, PIK3CA could bind to quercetin directly, which is validated by immunocoprecipitation. Therefore, the activation of PI3K/AKT phosphorylation was inhibited by quercetin and moreover the expressions of apoptotic proteins caspase-3 and Bcl2-Associated X protein (BAX) were up-regulated. In conclusion, the potential mechanism of nightshade lies in the activation of the PI3K/AKT signaling pathway inhibited by quercetin, thus promoting apoptosis of colon cancer cells for colon cancer treatment.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Study on the molecular mechanism of nightshade in the treatment of colon cancer

Song

Cheng

et al. 2022

Bioengineered

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“…The TCM is a complex system composed of many compounds, showing complicated pharmacological activities (synergistic or antagonistic) with multiple targets and pathways. Network pharmacology meets the core concept of the holistic philosophy of TCM and has been widely used to investigate the detailed compound-target and target-pathway relationships of herbs used for the treatment of complicated diseases [ 24 – 26 ]. In the current study, two key herbal medicines ( Salvia miltiorrhiza and Astragalus mongholicus ), corresponding to 8 key chemical components, were identified, and these chemical components were found to target 27 genes and major gene-regulated pathways.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological Network Reveals the Active Mechanism of Qi‐Replenishing, Spleen‐Strengthening, Phlegm‐Dispelling, and Blood‐Nourishing Fufang on Coronary Heart Disease

Zhang

Liu

Zheng

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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This study aimed to investigate the potential targets and pathways of qi-replenishing, spleen-strengthening, phlegm-dispelling, and blood-nourishing Fufang in the treatment of coronary heart disease (CHD). The composition of Fufang was identified, followed by screening of the active components using ADME. The targets of active components were predicted and screened based on the TCMSP and BATMAN databases and were cross-validated using the CTD database and DisGeNET. A functional enrichment analysis was performed using the ClueGO + CluePedia plugins and clusterProfiler in the R package. The protein-protein interaction (PPI) network was constructed using the STRING database and Cytoscape. Finally, a pharmacological network was constructed. A total of 27 overlapping targets were obtained after cross-validation. ALB, IL-6, and TNF were the hub genes in the PPI network. The pharmacological network included 59 nodes and 189 relation pairs. Among the 59 nodes, there were 2 herbal medicine nodes (Salvia miltiorrhiza and Astragalus mongholicus), 8 chemical component nodes (magnesium lithospermate B, neocryptotanshinone II, heteratisine, daphneolone, tanshinone IIA, tanshinone IIB, soyasapogenol B, and astragaloside II), 27 target protein nodes (such as ALB, TNF, IL-6, NFKB1, APOA1, APOA2, CYP1A1, and CYP1A2), and 22 pathway nodes (such as the toll-like receptor signaling pathway, IL-17 signaling pathway, and TNF signaling pathway). Therefore, we found that the genes TNF, IL-6, NFKB1, ALB, CYP1A1, CYP1A2, APOA1, and APOA2 might be important targets of the key active compounds neocryptotanshinone II and astragaloside II. These genes targeted by the key active compounds might regulate inflammation-related pathways and the level of albumin and cholesterol in CHD.

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“…Meanwhile, proteins contained in deer antlers protect against oxidative stress and in ammation [3,12]. Network pharmacology, which is based on the concept of a multilevel and multiangle interaction network among diseases, genes, targets, and drugs, can observe the interventional mechanism and in uence of drugs on the disease network systematically and comprehensively [13]. It can generate complex networks of interactions according to target molecules, biological functions, and bioactive compounds to clarify the mechanism of action of TCM prescriptions at the molecular level [14].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Intervertebral Disc Degeneration Treatment with Deer Antlers Based on Network Pharmacology and Molecular Docking

Weng

Lin

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. With the aging of the population, the prevalence of IVDD increases preoperatively. How to better treat IVDD has become an important clinical issue. Deer antlers proved to have a great effect on the treatment of IVDD in many studies, but the molecular mechanism has not been clarified. Objective. To investigate the molecular mechanism and target of deer antlers in the treatment of IVDD. Methods. Compounds from deer antlers were collected and targets were predicted using HERB, TCMSP, TCMID, SwissADME, and SwissTargetPrediction. Collection of disease targets for IVDD was done using GeneCards, TTD, DrugBank, DisGeNET, and OMIM. Cytoscape 3.7.2, AutoDock Vina (v1.1.2), and R software were used for data analysis and the construction of network diagrams. Results. A total of 5 active compounds from deer antlers were screened and 104 therapeutic targets were predicted. A total of 1023 IVDD disease targets were collected. Subsequently, PPI network prediction analysis was performed for disease and treatment targets, and 112 core targets were collected after screening. After obtaining the core target, we used the clusterProfiler software package of R software to carry out GO and KEGG enrichment analyses for the core target and plot the bubble maps. According to the GO enrichment results, the main biological processes of IVDD treatment by deer antlers lie in the rhythmic process, mRNA catabolic process, and G1/S transition of the mitotic cell cycle. KEGG results were mainly related to the PI3K-Akt signaling pathway, thyroid hormone signaling pathway, and Notch signaling pathway. Molecular docking results showed that estrone had the best docking results on ESR1. Conclusion. Deer antlers are rich in various compounds that can prevent the development of IVDD by upregulating the PI3K-Akt signaling pathway and Notch signaling pathway. Its key compounds estradiol and estrone can reduce the inflammatory response and oxidative stress in tissues and organs, thus slowing down the progression of IVDD. Estrone, the active compound in deer antlers, was found by molecular docking to have good results against ESR1, the target of the disease, which may be a potential site for drug therapy.

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<p>Network pharmacology study on the active components of <em>Pterocypsela elata</em> and the mechanism of their effect against cerebral ischemia</p>

Cited by 21 publications

References 18 publications

RETRACTED ARTICLE: Study on the molecular mechanism of nightshade in the treatment of colon cancer

RETRACTED ARTICLE: Study on the molecular mechanism of nightshade in the treatment of colon cancer

Pharmacological Network Reveals the Active Mechanism of Qi‐Replenishing, Spleen‐Strengthening, Phlegm‐Dispelling, and Blood‐Nourishing Fufang on Coronary Heart Disease

Mechanisms of Intervertebral Disc Degeneration Treatment with Deer Antlers Based on Network Pharmacology and Molecular Docking

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