<p>New serum biomarker identification and analysis by mass spectrometry in cervical precancerous lesion and acute cervicitis in South China</p>

Abstract

Background: According to the statistics of WHO/IARC, cervical cancer (CC) has become the fourth malignant cancer of female worldwide and it is one of the main causes of death of women in developing countries. Purpose: Potential plasma and metabolic biomarkers for CC precancerous lesions and cervicitis were indicated by LC-MS techniques, and their underlying mechanisms and functions were analyzed. Methods: Plasma samples were selected from healthy people (control), low-grade squamous intraepithelial lesions (LSIL), high-grade squamous intraepithelial lesions (HSIL), CC, and post-treatment patients. All polypeptide types and sequences were detected by LC-MS/MS and the results were normalized by using Pareto-scaling. Potential metabolic biomarkers were screened by applying MetaboAnalyst 4.0 software and XCMS software, and analysis of variance and enrichment analysis were performed. Metabolic pathway analysis and functional enrichment analysis were used to further investigate the significance and pathological mechanisms of potential biomarkers. Results: Compared with healthy people, 9 differentially expressed metabolites were screened, 4 of which were up-regulated and 5 were down-regulated. LSIL group screened 7 differentially expressed metabolites, 5 of which were up-regulated and 2 were down-regulated; CC group screened 12 differentially expressed metabolites were screened, of which 9 were up-regulated and 3 were down-regulated. Eight differentially expressed metabolites were screened in the IF group, of which 5 showed up-regulation and 3 showed down-regulation. In functional enrichment analysis, differential metabolism was found to be associated with addition and coagulation cascades. Among all potential biomarkers, 2-amino-3-methyl-1-butanol, L-carnitine, Asn Asn Gln Arg, Ala Cys Ser Trp, Soladulcidine, Ala Ile Gln Arg, 2-amino-3 -Methyl-1-butanol, L-carnitine, Asn Asn Gln Arg, Ala Cys Ser Trp, Soladulcidine, Ala Ile Gln Arg can be used as predictors of precancerous lesions at different stages of CC. Among all biomarkers, 6α-fluoro-11β1,17-dihydroxypren-4-ene-3,20-dione has higher expression in the CC and HSIL groups and lower expression in the treatment group. Conclusion: By applying molecular markers to assess the progression of the disease, the accuracy and specificity of the diagnosis can be improved, which has certain prospects in clinical applications.

Plasma-Based Metabolomics Profiling of High-Risk Human Papillomavirus and their Emerging Roles in the Progression of Cervical Cancer

“…HR-HPV is a potent human carcinogen, and persistent HR-HPV infection is a necessary risk factor for the cervix and cervical cancer [ 24 ]. It induces epithelial cell malignant transformation and suppresses the immune response by encoding oncoproteins [ 25 ].…”

“…This result suggested LysoPC and PC may also correlate with HR-HPV-induced oncogenesis, consistent with a previous study concluding PC and LysoPC were potential biomarkers for cervical cancer [ 49 ]. LysoPC originates from the cleavage of PC, the main component of oxidatively damaged low-density lipoprotein, was reported to aggregate inflammation and associated with the invasion, metastasis, and prognosis of tumors [ 24 ]. In addition, emerging evidence has indicated that enzymes participating in key lipid metabolism are potential therapeutic targets because they either inhibit lipid synthesis or stimulate their degradation [ 10 ].…”

Differences between novel hybrid mode flaps and traditional perforator flaps at the level of metabolites using LC–MS

“…Annotation of metabolites was conducted by matching samples with the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the Human Metabolome Database (HMDB). Data sets were analyzed using principal component analysis (PCA; Ramadan et al, 2006) and the VIP obtained by partial least squares discriminant analysis (PLS‐DA; Souza et al, 2012) was used to analyze the expression of the metabolites (filter condition VIP ≥ 1.0) using MetaboAnalyst 4.0 (Qiu et al, 2019). Preliminary screening criteria were as follows: fold change (FC) >2 times or <0.5, P < 0.05 and VIP value ≥1.…”

Synthesis and Biological Activity of Penaresidins A and B, Penazetidine A, and Related Analogues

“…Penaresidin A (1) was identified as a promising biomarker for cervical cancer, which is one of the main causes of death for women in developing countries. 7 To determine the relative stereochemistry of the (2S,3R,4S) protein kinase inhibitor 6 in vitro cytotoxicity against human and murine cell lines 11 :…”