<p>Occurrence of Multi-Drug Resistant <em>Escherichia Coli</em> and <em>Escherichia Coli</em> O157:H7 in Meat and Swab Samples of Various Contact Surfaces at Abattoir and Butcher Shops in Jimma Town, Southwest District of Ethiopia</p>

Abstract

Background Raw meat is one of the commonly consumed traditional diets in Ethiopia. However, unhygienic processing and distribution practices are risky for contamination of meat leading to human infection. This study was conducted to assess the presence of multi-drug resistant E. coli with special emphasis on E. coli O157:H7 from meat of cattle and swab samples at abattoir houses and butcher shops in Jimma town, Southwest district of Ethiopia. Methodology A cross-sectional descriptive study was conducted from April to July, 2018. The isolation and identification processes passed through enrichment of samples with modified tryptone soy broth (mTSB), streaked onto MacConkey agar and Cefixime-tellurite sorbitol MacConkey agar, biochemical testing (indole and TSI), followed by latex agglutination testing. Results Out of 505 samples, 102 (20.2%) and 27 (5.4%) were positive for E. coli and E. coli O157:H7, respectively. Of these, 55 (19.3%) and 47 (21.4%) of E. coli and 17 (6.0%) and 10 (4.5%) of E. coli O157:H7 were isolated from the abattoir and butcher shop samples, respectively. A significant difference in the occurrences was observed among sample sources. Antimicrobial susceptibility test results showed that, 92.2% to 96.1% of E. coli and 85.5% to 96.3% of E. coli O157:H7 were susceptible to third generation cephalosporin, ciprofloxacin, gentamycin, kanamycin, streptomycin, and chloramphenicol. About 91.2% and 97.1% of E. coli and 88.9% and 92.6% of E. coli 0157:H7 were resistant to ampicillin and erythromycin, respectively. A total of 57 (44.2%) E. coli and E. coli O157:H7 isolates were resistant to three or more classes of antibiotics. All abattoir and butcher shop workers did not have any formal education or training certificates on food safety, and unhygienic practices were also observed. Conclusion The presence of E. coli and E. coli O157:H7 including multi-drug resistant isolates in raw meat highlights how the current meat processing and distribution practice was unhygienic. Therefore, strategies in the prevention and control of food-borne infections that could be caused by multi-drug resistant strains will depend greatly on hygienic processing and distribution practices of meat.

High prevalence and antimicrobial susceptibility pattern of salmonella species and extended-spectrum β-lactamase producing Escherichia coli from raw cattle meat at butcher houses in Hawassa city, Sidama regional state, Ethiopia

“…This variation might be due to the differences in the hygienic and sanitary practices in the butcher shops especially poor hand washing practices. Studies conducted in Malaysia [7], Ghana [33] and Jimma [34] showed the prevalence of salmonella species to be 10%, 30%, and 11.3% respectively which were higher than the current study. This difference might be due to hygiene and sanitation practice, methodology differences in sample collection and processing procedure.…”

Isolation, characterization, and antimicrobial susceptibility pattern of Escherichia coli O157:H7 from foods of bovine origin in Mekelle, Tigray, Ethiopia

“…This study revealed an overall positivity rate of 24.6% for E. coli . This finding was in line with the reports of Bedasa et al ( 57 ) (20%), Sebsibe and Asfaw ( 58 ) (20.2%), Messele et al ( 59 ) (21.6%), Tadese et al ( 60 ) (23.4%), Abebe et al ( 61 ) (23.7%), Haileselassie et al ( 10 ) (27.3%), and Haftu et al ( 62 ) (27.3%). It was higher than the reports of Kumar et al ( 63 ) (8.15%), Yakubu et al ( 64 ) (9.23%), Atnafie et al ( 65 ) (12.38%), Mohammed et al ( 66 ) (15.89%), and Ababu et al ( 67 ) (19%).…”

Escherichia coli O157 : H7 from Food of Animal Origin in Arsi: Occurrence at Catering Establishments and Antimicrobial Susceptibility Profile

“…Antibiotic resistance in E. coli O157 : H7 has been increasingly noted over the last 20 years [ 24 ]. A recent study revealed that a higher incidence rate of E. coli O157 : H7 multidrug resistance, to more than two antimicrobial agents, was observed in Ethiopia [ 9 , 25 – 27 ].…”