<p>Overexpression of COL24A1 in Hepatocellular Carcinoma Predicts Poor Prognosis: A Study Based on Multiple Databases, Clinical Samples and Cell Lines</p>

Abstract

Purpose: Hepatocellular carcinoma (HCC) ranks as the second leading cause of cancerrelated deaths worldwide and its incidence tends to increase globally. Current biomarkers can hardly reflect diagnostic significance and therapeutic efficiency accurately. The role of collagen 24A1 (COL24A1) in HCC, as a member of the collagen family, remains unclear and needs to be proved. Methods: HCC-associated RNA sequencing datasets were obtained from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC) and Oncomine databases. R language was used to screen and analyze the differential expression genes (DEGs). Gene Ontology (GO) pathway analyses of DEGs were carried out using g:profiler. Then, the STRING database and cytoscape software were applied to estimate the correlations between DEGs. GEPIA and HCCDB database were used to verify and analyze these results. Finally, qPCR and immunohistochemistry staining on clinical samples and HCC cell lines were conducted to further verify the above results. Results: The expression of COL24A1 in HCC tissues was significantly higher than that in normal hepatic (NH) tissues. The analysis of TCGA and Oncomine database samples proved that COL24A1 expression was connected with tumor grade, tumor stage and pathologic stage. Meanwhile, the overall survival (OS) curve also validated that high expression of COL24A1 indicated poor prognosis. And the above results have been verified in our clinical samples and HCC cell lines. Furthermore, there is a significant increase of the expression of COL24A1 in various tumors. COL24A1, together with other interactive proteins, has a significant effect on the prognosis of HCC. Conclusion: Overall, COL24A1 may be an oncogene in the development of HCC. Also, the overexpression of COL24A1 in HCC may help diagnose HCC and evaluate prognosis.

High expression of TOP2A in hepatocellular carcinoma is associated with disease progression and poor prognosis

“…In addition, efforts to identify new effective prognostic biomarkers for HCC are ongoing. According to previous reports, high expression levels of collagen type XXIV alpha 1 chain ( 43 ), ubiquilin 2 ( 44 ), sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1 ( 45 ), acyl-CoA synthetase long chain family member 4 ( 46 ) and YTH N6-methyladenosine RNA binding protein 1 ( 47 ) are associated with the progression of HCC. Another study has demonstrated that in addition to the American Joint Committee on Cancer staging system, a long non-coding RNA-based risk score is another important factor affecting the overall survival of patients with HCC ( 48 ).…”

LncRNA SATB2-AS1 overexpression represses the development of hepatocellular carcinoma through regulating the miR-3678-3p/GRIM-19 axis

“…HCC is among the most lethal tumors globally, and its morbidity and mortality are still on the rise [ 1 , 5 ]. Moreover, HCC is a chemotherapy-resistant tumor with a worse survival rate [ 19 ].…”

“…However, due to the poor efficacy of the AFP test and the high dependence of the US on operators, the efficiency of early screening and diagnosis is not ideal. Accordingly, advanced HCC patients have a poor prognosis [ 5 ]. Hence, it’s vital to explore the pathogenesis of HCC and search for new diagnostic markers and identify useful therapeutic targets.…”

Network analysis of hepatocellular carcinoma liquid biopsies augmented by single-cell sequencing data

“…Similarly, we found that there were more male participants in the HCC group compared to the healthy control group. When we analyzed the most interconnected and highly correlated members of the modules which highly correlated with disease state, we found numerous genes with known association with HCC, including IMPDH2 ( He et al, 2018 ), COL24A1 ( Yan et al, 2020 ), RACK1 ( Cao et al, 2019 ), SNRPD2 ( Liu et al, 2022 ) and the hub genes RPL5 and TLK1 ( Segura-Bayona et al, 2020 ; Ye et al, 2022 ). A more comprehensive list of such genes can be found in Supplementary Table S1 .…”