<p>Potential Antioxidant and Wound Healing Effect of Nano-Liposol with High Loading Amount of Astaxanthin</p>

Abstract

Background: Astaxanthin (ASTA), a carotenoid, is a strong antioxidant. However, its application in functional foods, pharmaceuticals, and cosmetics remains limited due to its low aqueous solubility and stability. Several different encapsulating materials have been used to improve the stability and bioavailability of ASTA; however, the currently investigated nano-carriers for ASTA require additional improvements with regard to their loading capacity and stability. Methods: In this study, we developed lecithin nano-liposol (Lec NS) as a novel carrier of ASTA using a simple emulsion evaporation method. The physicochemical characteristics including hydrodynamic diameter, polydispersity index, surface charge and morphology were analyzed by DLS and TEM. The antioxidant activity of the ASTA-loaded Lec NS (ASTA@Lec NS) was evaluated using a DPPH radical scavenging assay and in vitro antioxidant assay. The study of in vitro wound healing efficacy was carried out to observe the beneficial effect of antioxidant activity of ASTA@Lec NS on cell migration. Results: ASTA@Lec NS showed improved stability and efficacy owing to improved aqueous solubility of ASTA inside Lec NS. Both in situ and in vitro antioxidant activities of ASTA@Lec NS were higher than that of bare ASTA and Lec NS. It also exhibited strong wound healing efficacy by regulation of ROS level in in vitro cell model. Conclusion: This study revealed that the encapsulation of ASTA into Lec NS using a wet phase transfer enhanced its physiological stability and bioavailability for effective scavenging of reactive oxygen species.

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“…All reaction mixtures were left in the dark at room temperature for 1 h, and the absorbance of the mixture was measured at a wavelength of 515 nm using a microplate reader (VICTOR X5, PerkinElmer, Singapore, Republic of Singapore). Antioxidant activity was calculated using the following equation [ 33 , 34 ]:…”

Ultrasmall Coordination Polymers for Alleviating ROS-Mediated Inflammatory and Realizing Neuroprotection against Parkinson’s Disease

“…In the DPPH assay, a solution of DPPH in ethanol (final concentration: 62.5 M) was combined with a range of Fe-Cur NCPs concentrations (0, 1.5625, 3.125, 6.25, 12.5, and 25 μ g/mL). After mixing completely, this mixture was placed into incubation (30 min and room temperature), following which absorption at 510 nm was measured, and DPPH scavenging activity was assessed as follows: DPPH scavenging ability (%) = (A DPPH -A sample )/A DPPH ) ∗ 100%, where A DPPH and A Sample represent the DPPH absorbance in the absence of additional treatment and after the addition of Fe-Cur NCPs, respectively, [ 40 ].…”

Vesicular Nanocarriers for Phytocompounds in Wound Care: Preparation and Characterization

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