<p>Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant<em> Staphylococcus aureus</em> and<em> Klebsiella pneumoniae</em></p>

Abstract

Background: Infections caused by drug resistant bacteria are a major health concern worldwide and have prompted scientists to carry out efforts to overcome this challenge. Researchers and pharmaceutical companies are trying to develop new kinds of antimicrobial agents by using different physical and chemical methods to overcome these problems. Materials and methods: In the present study, rifampicin conjugated silver (Rif-Ag) nanoparticles have successfully been synthesized using a chemical method. Characterization of the nanoparticles was performed using a UV-Vis spectrophotometer, FTIR, SEM, TEM, and AFM. Results: The AFM, SEM, and TEM results showed that the average particle size of Rif-Ag nanoparticles was about 15–18±4 nm. The FTIR spectra revealed the conjugation of –NH 2 and –OH functional moiety with silver nanoparticles surface. Considering the penetrating power of rifampicin, the free drug is compared with synthesized nanoparticle for antimicrobial, biofilm inhibition, and eradication potential. Synthesized nanoparticles were found to be significantly active as compared to drug alone. Conclusion: This study has shown greater biofilm inhibitory and eradicating potential against methicillin resistant Staphylococcus aureus and Klebsiella pneumoniae , as evident by crystal violet, MTT staining, and microscopic analysis. So, it will be further modified, and studies for the mechanism of action are needed.

Anti-biofilm and Antibacterial Activities of Silver Nanoparticles Synthesized by the Reducing Activity of Phytoconstituents Present in the Indian Medicinal Plants

“…The current results also revealed that the tested bacteria are highly sensitive to GL-AgNPs, BR-AgNPs, and SA-AgNPs, suggesting that the complex biofilm signaling mechanism could also be associated with cell survival. Recently, research has been conducted on conjugating the antibiotic, rifampicin, with chemically synthesized AgNPs for use in combatting biofilm formation by methicillin resistant S. aureus and Klebsiella pneumoniae (Farooq et al, 2019).…”

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

“…Consistent with our results, previous studies have shown efficient effect of AgNPs in inhibiting biofilms produced by different microorganisms 70 , 71 . Different investigators have evaluated the ability of silver nanoparticles to inhibit biofilm formation by A. baumannii , Pseudomonas aeruginosa (synergy with tobramycin) , Staphylococcus aureus (such as synergy with vancomycin), Escherichia coli and Klebsiella pneumonia at similar concentrations 23 , 72 – 78 .…”

Nanoparticles as therapeutic options for treating multidrug-resistant bacteria: research progress, challenges, and prospects

“…Mohamed (2020) reported that AuNps conjugated with ampicillin/amoxicillin showed significant antibacterial activity and was biocompatible with treated cells. Farooq et al (2019) showed that rifampicin conjugated AgNps produced anti-biofilm activity against MRSA and K. pneumonia. Surwade et al (2019) reported that AgNps combined with ampicillin showed very strong synergistic effects against MRSA.…”