<p>Simultaneous Detection of VEGF and CEA by Time-Resolved Chemiluminescence Enzyme-Linked Aptamer Assay</p>

Abstract

Background As two important tumor markers, vascular endothelial growth factor (VEGF) and carcinoembryonic antigen (CEA) have a great value for clinical application in the early diagnosis of cancer. Due to the complex composition of biological samples, the results from combined detection of CEA and VEGF are often taken as a comprehensive indicator in order to make an accurate judgment on a disease. However, most of the current methods can only be used to detect the content of one biomarker. Therefore, it is necessary to explore a simple, rapid, low-cost, and highly sensitive method for the simultaneous detection of CEA and VEGF. Methods Based on specific aptamers and magnetic separation, a time-resolved chemiluminescence enzyme-linked aptamer assay was developed for the simultaneous detections of CEA and VEGF in serum samples. Results Under the optimal conditions, the linear range of the calibration curve for VEGF was from 0.5 to 80 ng mL −1 , and the limit of detection was 0.1 ng mL −1 . The linear range of the calibration curve for CEA was 0.5 to 160 ng mL −1 , and the limit of detection was 0.1 ng mL −1 . The established method was applied to detect VEGF and CEA in serum samples. The results were consistent with those of commercial kits. Conclusion The method has high sensitivity and can quickly obtain accurate results, which could greatly improve the measurement efficiency, reduce the cost, and also reduce the volume of sample consumed. It can be seen that the method established in this study has important application value and broad application prospect in clinical diagnosis.

Application of Aptamer-based Biosensors in Early Diagnosis of Gastric Cancer: A Promising Method for Early Detection

“…Several studies have generated some aptamers against CEA and HER2, but the non-specificity of these markers for GC has led to new studies using a GC cell line to improve their specificity. [29][30][31][32][33] Using high-throughput sequencing SELEX, six aptamers were selected from a huge library against CEA, CA50, and CA72-4 in the GC adenocarcinoma AGS cell line. 34 The fluorescent signal of the aptamers was comparable to traditional antibody detection, suggesting that aptamers could be an effective alternative to antibody-based immunoassays.…”

Recent Advances in Nanomaterial‐based Optical Biosensors as Potential Point‐of‐Care Testing (PoCT) Probes in Carcinoembryonic Antigen Detection

“…Man et al . in the luminol‐based system [92c] . The MBs are modified with streptavidin so that the biotinylated single‐stranded (ss)DNA can form interaction with streptavidin via hydrogen bonding [96] .…”

“…Besides, the MBs are crucial in separating the unreacted capture antibody from the reacted ones in order to reduce false detection. Herein, the catalyst that mediates the oxidation of luminol is the natural enzyme, HRP [92c] …”

Construction of Liquid Crystal-Based Sensors Using Enzyme-Linked Dual-Functional Nucleic Acid on Magnetic Beads

“…Among them, the enzyme-linked aptamer assay (ELAA) is particularly popular. 22 In ELAA, the biotin-labeled aptamer is usually conjugated with streptavidin-labeled horseradish peroxidase (SA-HRP). Alternatively, the enzymes can be covalently conjugated to the aptamers, but these methods are usually complex.…”