<p>Standardized <em>Arrabidaea chica</em> Extract Shows Cytoprotective Effects in Zoledronic Acid-Treated Fibroblasts and Osteoblasts</p>

Abstract

Introduction: Osteonecrosis of the jaw is a condition associated with intraoral ulceration and bone necrosis induced by antiresorptive medications, such as zoledronic acid, a bisphosphonate. Previous data on Arrabidaea chica (H&B.) Verlot wound healing activity prompted the study reported herein on A. chica standardized hydro alcoholic extract in vitro cytoprotective activity data on epithelial and osteoblastic cells exposed to zoledronic acid (ZA). Methods: Primary human gingival fibroblasts and murine pre-osteoblasts were treated with ZA 10 µM together with 5 or 10 µg.mL −1 A. chica extract for 24h and 48 h. At both times, cells were submitted to viability assay and caspase 3/7 activation evaluation. Statistical analysis used one-way ANOVA and p=0.05. Results: In cell viability assay, a drastic damage effect of ZA appeared after 48 h in both epithelial (55.8%) and pre-osteoblastic cells (39.7%). When treated with ZA in combination with A. chica extract, cells showed higher viability values: 74.1%-82.3% for fibroblasts and 66% for pre-osteoblasts. Furthermore, the combined treatment presented lower caspase 3/7 activation in fibroblasts and pre-osteoblasts. Conclusion: At low concentrations, A. chica extract showed promising cytoprotective effects against ZA-induced damage actions; however, further in vitro and in vivo studies are required to establish the mechanism of action.

In Vitro Cytotoxicity of Antiresorptive and Antiangiogenic Compounds on Oral Tissues Contributing to MRONJ: Systematic Review

“…Scheper et al [ 51 ] showed that oral tissues treated with ZA had elevated expression of genes involved in the intrinsic (TNF, TRAF, death domain) and extrinsic (BCL, IAP, Caspase) apoptotic pathways, potentially explaining the increased rate of apoptosis observed in multiple studies. Increased caspase-3 and 9 activity, in cells treated with ZA and PA, as well as increased ROS production in N-BP-treated cells likely induced apoptosis in these cells [ 40 , 51 , 65 , 66 ].…”

Zoledronate repositioning as a potential trypanocidal drug. Trypanosoma cruzi HPRT an alternative target to be considered

“…New formulations of alendronate or zoledronate by encapsulation in liposomes or nanoparticles are being investigated to increase their effectiveness and reduce the doses used [34]. On the other hand, intravenous administration of zoledronate [35] and the combination of zoledronate with other drugs [36,37] are being tested in order to reduce its adverse effects. These studies can be inspiring for stimulating research on the treatment of other diseases.…”

Ação do extrato de Arrabidaea chica em biofilmes de Candida albicans, efeito citotóxico em fibroblastos e queratinócitos, e nas propriedades físico/mecânicas de resina de poli(metilmetacrilato)

“…Foram encontrados os compostos majoritários 6,7,3′,4′-tetrahydroxy-5-methoxyflavylium, 6,7,4′-trihydroxy-5-methoxyflavylium, e carajurin após análise por HPLCDAD que podem justificar a atividade do AchE. O conteúdo de carajurin no AchE representa 6.51%±0.07% do seu total (Wiziack Zago et al, 2020)…”

“…Na literatura, diversos efeitos biológicos e farmacológicos da A. chica foram relatados, tendo destaque o seu potencial antimicrobiano demonstrando ação antifúngica contra algumas espécies de Candida (Hofling et al, 2010, Mafioletiet et al, 2013, Wiziack Zago et al, 2020, eficácia quando utilizada na cicatrização de feridas, efeito anti-inflamatório e atividade antioxidante (Giordani et al, 2015, Jorge et al, 2008, Medeiros et al, 2011, Aro et al, 2012, Oliveira et al, 2009, sendo utilizada geralmente na forma de extrato hidroalcoólico (Santos et al, 2013) ou diclorometano (Hofling et al, 2010).…”