&lt;p&gt;The First Egyptian Report Showing the Co-Existence of &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;NDM-25&lt;/sub&gt;, &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;OXA-23&lt;/sub&gt;, &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;OXA-181&lt;/sub&gt;, and &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;GES-1&lt;/sub&gt; Among Carbapenem-Resistant &lt;em&gt;K. pneumoniae&lt;/em&gt; Clinical Isolates Genotyped by BOX-PCR&lt;/p&gt;

El-Badawy, Mohamed F.; El‐Far, Shaymaa W.; Althobaiti, Shaker S; Abou-Elazm, Fatma I; Shohayeb, Mohamed

doi:10.2147/idr.s244064

Cited by 42 publications

(35 citation statements)

References 53 publications

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“… 46 Although there is a marked increase in the resistance of amikacin, colistin, and tigecycline in recent studies in Egypt, they are still effective against carbapenem-resistant Enterobacteriaceae. 37 , 39 , 47 Molecular typing is a useful tool for determining the genetic relatedness of bacteria. This is particularly important in the investigation of the epidemiology of several bacterial infectious diseases accompanied by HAI.…”

Section: Discussionmentioning

confidence: 99%

“…Similar to our study, recent two studies were conducted also in Egypt that reported the prevalence of diverse clones from CRKP using the PFGE and BOX PCR as molecular typing methods, respectively. 37 , 49 …”

Section: Discussionmentioning

confidence: 99%

“…35 As reported in other previous studies in Egypt, our study also concluded that the predominant species among the isolated CRE was MDR K. pneumoniae, which represents a frightening causative agent of different types of infections among hospitalized pediatric cancer patients. [35][36][37][38] It was reported that class D carbapenemases (bla OXA-48 = 58%) are predominant in K. pneumoniae followed by the bla NDM-1 gene, in several studies conducted in different countries notably in the Mediterranean region including Spain, France, Turkey, Tunisia and Egypt. 20,21 Furthermore, the majority (41.8%) of the K. pneumoniae isolates in the current study harbored one or more carbapenem resistance determinants (Figure 2) that agreed with previously reported studies.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

Osama¹,

El-Mahallawy²,

Mansour³

et al. 2021

IDR

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Purpose Healthcare-associated infections caused by multi-drug-resistant (MDR) pathogens are a global threat. We aim to assess the clonal relatedness among carbapenemase-producing Klebsiella pneumoniae (CPKP) strains infecting Egyptian pediatric cancer patients. Materials and Methods Identification and antimicrobial susceptibility testing of 149 Gram-negative isolates obtained from pediatric cancer patients were performed by VITEK 2. Genes encoding carbapenemases and extended-spectrum β-lactamases were detected by PCR and verified by DNA sequencing of representative samples. The transferability of the plasmids harboring bla OXA-48 , from representative clinical samples, was evaluated by performing a conjugation experiment followed by PCR and MIC shift determination. Clonal relationships among the bla OXA-48 -harboring K. pneumoniae isolates were determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR and pulsed-field gel electrophoresis (PFGE). Results Carbapenem resistance was observed in 59% of the isolates. The most prevalent species was K. pneumoniae (45.6%) and 57% of them were isolated from ICU. Fifty-nine % of the K. pneumoniae isolates were carbapenemase-producers and bla OXA-48 was detected in (58%) of them. One isolate co-harbored bla OXA-48, bla NDM-1 , and bla IMP-1 genes for the first time in Egypt. PCR and meropenem MIC shift confirmed the success of the transferability of representative plasmids to E. coli K12. ERIC and PFGE identified 93% and 100% of the K. pneumoniae with a similarity coefficient ≥85%, respectively, including strains with indistinguishable patterns, suggesting possible clonal dissemination. Conclusion Our findings underline the dissemination of diverse clones of MDR CPKP among Egyptian pediatric cancer patients. Hence, routine molecular characterizations followed by strict implementation of infection control measures are crucial to tackling this threat.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

Osama¹,

El-Mahallawy²,

Mansour³

et al. 2021

IDR

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“…The existence of OXA-23 among CRKP clinical isolates in Egypt was also reported recently. 23 This suggests that we should be alert to the spread of OXA-23 in K. pneumoniae , which is only prevalent in Acinetobacter baumannii . 23 However, MLST schemes corresponding to CPKP strains have remained imprecisely defined.…”

Section: Discussionmentioning

confidence: 99%

“… 23 This suggests that we should be alert to the spread of OXA-23 in K. pneumoniae , which is only prevalent in Acinetobacter baumannii . 23 However, MLST schemes corresponding to CPKP strains have remained imprecisely defined. 9 , 10 cgMLST analysis may eliminate spurious MLST associations.…”

Section: Discussionmentioning

confidence: 99%

Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China

Zhang¹,

Li²,

Lu³

et al. 2021

IDR

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Background ST11 is the most prevalent sequence type of clinical Klebsiella pneumoniae in China. Methods We investigated the characteristics of the ST11 subclones using core genome multi-locus sequence typing (cgMLST). Ninety-three carbapenemase-producing K. pneumoniae isolates were collected at Shenzhen People’s Hospital. Then, whole-genome sequencing and cgMLST were used to discriminate apparent subclones within the ST11 group. Results We analyzed the prevalence and genetic relationships of these subclones. ST11 and K. pneumoniae carbapenemase (KPC-2) were the predominant genotype and carbapenemase, respectively, in the clinical carbapenemase-producing K. pneumoniae strains. cgMLST scheme genotyping divided the ST11 group into two clades across seven complex types (CTs). CT1313 was the most prevalent subclone. The deletion of galF and a high frequency of SNPs in genes associated with the stress- and SOS-responses were found in CT1291 and CT2405 over time, respectively. Conclusion Our results indicated that the subclones of the ST11 group had different patterns of prevalence. Highly discriminatory genotyping techniques, such as cgMLST scheme, should be used in further molecular epidemiology investigations.

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High Prevalence of blaNDM Among Carbapenem Non-Susceptible Klebsiella pneumoniae in a Tunisian Hospital First Report of blaNDM-9, blaKPC-20, and blaKPC-26 Genes

et al. 2023

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Fifty-four carbapenem non-susceptible Klebsiella pneumoniae (CNSKP) isolates were collected from a Tunisian hospital over a period of 13 consecutive months. Carbapenemase production and the prevalence of carbapenemase-encoding genes were investigated using combined-disk test (CDT), modified Carba NP (mCarba NP) test, and UV-spectrophotometry method complemented by PCR experiments and sequencing. Carbapenemase production was detected by the mCarba NP test and CDT in 92.59% and 96.29% of the 54 CNSKP isolates, respectively; while imipenem hydrolysis was detected using UVspectrophotometry in the crude extracts of 44 isolates. bla NDM, bla OXA-48-like , and bla KPC carbapenemase-encoding genes were found in 48, 31, and 22 isolates, respectively. Remarkably, bla NDM-9, bla KPC-20 , and bla KPC-26 genes were reported. The co-occurrence of carbapenemase-encoding genes in a single isolate was detected in 62.96% of the isolates. The analysis of clonal relationships between the isolates by pulsed field gel electrophoresis revealed that the majority of them were genetically unrelated. Our investigation provides molecular data on enzymatic mechanism of carbapenem non-susceptibility among 54 CNSKP showing the dominance of bla NDM, and comprises the first identification of bla NDM-9, bla KPC-20 , and bla KPC-26 genes in a Tunisia hospital.

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<p>The First Egyptian Report Showing the Co-Existence of <em>bla</em><sub>NDM-25</sub>, <em>bla</em><sub>OXA-23</sub>, <em>bla</em><sub>OXA-181</sub>, and <em>bla</em><sub>GES-1</sub> Among Carbapenem-Resistant <em>K. pneumoniae</em> Clinical Isolates Genotyped by BOX-PCR</p>

Cited by 42 publications

References 53 publications

Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

Expanding of ST11 Carbapenemase-Producing Klebsiella pneumoniae Subclones in a Chinese Hospital, Shenzhen, China

High Prevalence of blaNDM Among Carbapenem Non-Susceptible Klebsiella pneumoniae in a Tunisian Hospital First Report of blaNDM-9, blaKPC-20, and blaKPC-26 Genes

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