<p>The Multifunction Of miR-218-5p-Cx43 Axis In Breast Cancer</p>

Abstract

Background: Gemcitabine is proven to be the first-line standard treatment of breast cancers. Yet, little is known involving gemcitabine resistance and remains largely to be elucidated. Materials and methods: We evaluated the expression of Cx43 in gemcitabine-resistant cells and parental cells by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analyses. Dual-luciferase reporter assay was applied to examine the epigenetic regulator of Cx43. The role of miR-218-5p-Cx43 axis on cell cytotoxicity, cell proliferation, colony formation, chemoresistance and migration was detected via mammalian expression vector and small short RNA (shRNA) transfection in vitro. Results: In this study, we found that Cx43 expression levels were significantly lower in gemcitabine-resistant cells than in the parental cells. On deep investigation of the epigenetic regulation of Cx43, a few miRNA candidates targeting Cx43 were derived. Through dual-luciferase reporter assay, Cx43 was proved to be a direct target of miR-218-5p. Besides, qPCR, Western blot demonstrated an inverse correlation between miR-218-5p and Cx43 expression in breast cancer cells, thus forming the miR-218-5p-Cx43 axis. Notably, miR-218-5p-Cx43 axis was found to be involved in the process of gemcitabine chemoresistance, cell proliferation and migration in breast cancer cells. Conclusion: Our findings suggested that miR-218-5p-Cx43 axis was versatile and indicated significant potency in breast cancer cells. More importantly, miR-218-5p-Cx43 axis might be valuable in translational medicine, with therapeutic and prognostic information.

Perspectives on miRNAs Targeting DKK1 for Developing Hair Regeneration Therapy

“…miR-218 via its role in Wnt/β-catenin signaling represents a hot spot molecule in cancer studies, as dysregulation of this miRNA is strongly associated with different cancers [ 211 , 212 , 213 , 214 , 215 , 216 ]. Except for the cancers, miR-218-5p plays an essential role in the HF and skin development again through Wnt/β-catenin signaling by targeting and inhibiting SFRP2—the antagonist of this signaling pathway [ 76 ].…”

Long noncoding RNA MAGI2‐AS3/miR‐218‐5p/GDPD5/SEC61A1 axis drives cellular proliferation and migration and confers cisplatin resistance in nasopharyngeal carcinoma

“…Considering that overexpression of GDPD5 partly reversed the inhibiting effect of MAGI2‐AS3 silencing on NPC cellular progression, we aimed to preliminarily identify whether there were alternate targets for MAGI2‐AS3 in NPC. We kept our focus on the MAGI2‐AS3/miR‐218‐5p axis; accordingly, we referred to previous research and identified 20 mRNAs reported as miR‐218‐5p targets, namely Cx43, 25 LASP1, 26 TXNDC5, 27 , SHMT1, 28 TRAF1, TRAF2, TRAF5, 29 HMGB2, 30 HMGB1, 31 SEC61A1, 32 DDX21, 33 CD44, 34 DPH1, 35 CDK6, CCND1, 36 PRKCE, 37 SOST, SFRP2, 38 EGFR, 39 and TFF1 40 . We then aimed to confirm the correlation of these genes with MAGI2‐AS3.…”

MiR-218-5p Affects Subcutaneous Adipogenesis by Targeting ACSL1, a Novel Candidate for Pig Fat Deposition

“…MiR-218-5p is a highly conserved miRNA in mammals, which has been considered as a tumour suppressor by inhibiting cell proliferation, migration, growth, and metastasis of many cancers, including breast cancer, prostate cancer, and cervical cancer [ 29 , 30 , 31 ]. Additionally, many studies also found that miR-218-5p can regulate skin and hair development [ 32 , 33 ].…”