&lt;p&gt;The opposite role of alternatively spliced isoforms of LINC00477 in gastric cancer&lt;/p&gt;

Zhao, Hongchao; He, Yuanhang; Li, Haohao; Zhu, Ali; Ye, Yanwei; Liu, Guanghui; Zhao, Chunlin; Zhang, Xiefu

doi:10.2147/cmar.s202430

Cited by 14 publications

(12 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RIP assay is followed as previously described (Zhao et al, 2019). Briefly, 1 × 10 7 EOC cells were harvested, resuspended in nuclear isolation buffer (1.28 M sucrose, 40 mM Tris, pH 7.5, 20 mM MgCl 2 , 4% Triton X‐100) and kept on ice for at least 30 min with frequent mixing.…”

Section: Methodsmentioning

confidence: 99%

METTL3 regulates m6A in endometrioid epithelial ovarian cancer independently of METTl14 and WTAP

Liu

et al. 2020

Cell Biology International

View full text Add to dashboard Cite

N6‐methyladenosine (m6A) RNA methylation, one of the common RNA modifications, has been determined to execute crucial functions in tumorigenesis and cancer development. The m6A “writers” including methyltransferase like 3 (METTL3), METTL14, and Wilms tumor 1‐associated protein (WTAP) contribute to the m6A modification process initiation. However, the coordination of m6A methyltransferase complex is not fully understood in endometrioid epithelial ovarian cancer (EEOC). In this study, mRNA and protein levels of METTL3, METTL14, and WTAP were detected in 33 EEOC cases using quantitative polymerase chain reaction (qPCR), immunohistochemistry, and western blot analysis. The overall m6A methylation was detected by dot plot. The METTL3 expression and overall m6A level were elevated in EEOC tissues, while the expressions of METTL14 and WTAP have no significant difference in EEOC compared to the adjacent tissues. The expression of METTL3 was an independent factor that correlated with poor malignancy and survival of EEOC patients. Moreover, METTL3 knockdown in TOV‐112D and CRL‐11731D cells weakened the capability of cell proliferation and migration, and promoted cell apoptosis compared to negative control and cells with WTAP or METTL14 knockdown using CCK‐8 assay, transwell assay, wound healing assay, and TUNEL assay. Furthermore, METTL3 knockdown also reduced m6A enrichment of the genes associated with ovarian cancer including EIF3C, AXL, CSF‐1, FZD10 in TOV‐112D, and CRL‐11731D cells by RIP‐qPCR assay. Taken together, the high expressed METTL3 indicated poor malignancy and survival of EEOC via modulating the aberrant m6A RNA methylation. METTL3‐mediated m6A modification, independent of WTAP and METTL14, was considered as a novel mechanism underlying m6A modulation and a potential therapeutic target of EEOC.

show abstract

Section: Methodsmentioning

confidence: 99%

METTL3 regulates m6A in endometrioid epithelial ovarian cancer independently of METTl14 and WTAP

Liu

et al. 2020

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…RIPA is followed as previously described. 26 Briefly, 1 Â 10^7 H9C2 cells were harvested, resuspended in nuclear isolation buffer (1.28 M sucrose, 40 mM Tris pH 7.5, 20 mM MgCl 2 , 4% Triton Figure 6. The Association of NCBP3 with "5 0 -GAAGCUGCC-3 0 " Motif (A) The list of predicted RBPs for RNA motif.…”

Section: Ripamentioning

confidence: 99%

Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes

Shen

Zeng

Zhang

2020

Molecular Therapy - Nucleic Acids

View full text Add to dashboard Cite

Adult cardiac hypoxia as a crucial pathogenesis factor can induce detrimental effects on cardiac injury and dysfunction. The global transcriptome and translatome reflecting the cellular response to hypoxia have not yet been extensively studied in myocardium. In this study, we conducted RNA sequencing (RNA-seq) and ribosome profiling technique (polyribo-seq) in rat heart tissues and H9C2 cells exposed to different periods of hypoxia stress in vivo and in vitro. The temporal gene-expression profiling displayed the distinction of transcriptome and translatome, which were mainly concentrated in cell apoptosis, autophagy, DNA repair, angiogenesis, vascular process, and cardiac cell proliferation and differentiation. A large number of genes such as GNAI3, SEPT4, FANCL, BNIP3, TBX3, ESR2, PTGS2, KLF4, and ADRB2, whose transcript and translation levels are closely correlated, were identified to own a common RNA motif "5 0-GAAGCUGCC-3 0 " in 5 0 UTR. NCBP3 was further determined to recognize this RNA motif and facilitate translational process in myocardium under hypoxia stress. Taken together, our data show the close connection between alterations of transcriptome and translatome after hypoxia exposure, emphasizing the significance of translational regulation in related studies. The profiled molecular responses in current study may be valuable resources for advanced understanding of the mechanisms underlying hypoxia-induced effect on heart diseases.

show abstract

“…[35][36][37] For example, the alternative splicing of LINC00477 can produce two isoforms with different tumor suppression abilities. 38 The two functionally different transcripts in our studies indicate that alternative splicing at this gene locus may be vital in determining the fate of MSCs. Different microenvironments induce different forms of splicing, which eventually leads MSCs toward different differentiation outcomes.…”

Section: Discussionmentioning

confidence: 74%

Long noncoding RNA repressor of adipogenesis negatively regulates the adipogenic differentiation of mesenchymal stem cells through the hnRNP A1‐PTX3‐ERK axis

Pan

Xie

Cen

et al. 2020

Clinical & Translational Med

View full text Add to dashboard Cite

Background Mesenchymal stem cells (MSCs) are pluripotent stem cells that can differentiate via osteogenesis and adipogenesis. The mechanism underlying MSC lineage commitment still remains incompletely elucidated. Understanding the regulatory mechanism of MSC differentiation will help researchers induce MSCs toward specific lineages for clinical use. In this research, we intended to figure out the long noncoding RNA (lncRNA) that plays a central role in MSC fate determination and explore its application value in tissue engineering. Methods The expression pattern of lncRNAs during MSC osteogenesis/adipogenesis was detected by microarray and qRT‐PCR. Lentivirus and siRNAs were constructed to regulate the expression of lncRNA repressor of adipogenesis (ROA). MSC osteogenesis/adipogenesis was evaluated by western blot and alizarin red/oil red staining. An adipokine array was used to select the paracrine/autocrine factor PTX3, followed by RNA interference or recombinant human protein stimulation to confirm its function. The activation of signaling pathways was also detected by western blot, and a small molecule inhibitor, SCH772984, was used to inhibit the activation of the ERK pathway. The interaction between ROA and hnRNP A1 was detected by RNA pull‐down and RIP assays. Luciferase reporter and chromatin immunoprecipitation assays were used to confirm the binding of hnRNP A1 to the PTX3 promotor. Additionally, an in vivo adipogenesis experiment was conducted to evaluate the regulatory value of ROA in tissue engineering. Results In this study, we demonstrated that MSC adipogenesis is regulated by lncRNA ROA both in vitro and in vivo. Mechanistically, ROA inhibits MSC adipogenesis by downregulating the expression of the key autocrine/paracrine factor PTX3 and the downstream ERK pathway. This downregulation was achieved through transcription inhibition by impeding hnRNP A1 from binding to the promoter of PTX3. Conclusions ROA negatively regulates MSC adipogenesis through the hnRNP A1‐PTX3‐ERK axis. ROA may be an effective target for modulating MSCs in tissue engineering.

show abstract

<p>The opposite role of alternatively spliced isoforms of LINC00477 in gastric cancer</p>

Cited by 14 publications

References 26 publications

METTL3 regulates m6A in endometrioid epithelial ovarian cancer independently of METTl14 and WTAP

METTL3 regulates m6A in endometrioid epithelial ovarian cancer independently of METTl14 and WTAP

Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes

Long noncoding RNA repressor of adipogenesis negatively regulates the adipogenic differentiation of mesenchymal stem cells through the hnRNP A1‐PTX3‐ERK axis

Contact Info

Product

Resources

About