&lt;p&gt;The Predictive Value of microRNA-134 and microRNA-1233 for the Early Diagnosis of Acute Exacerbation of Chronic Obstructive Pulmonary Disease with Acute Pulmonary Embolism&lt;/p&gt;

Peng, Ling; Han, Li; Li, Xiaoning; Miao, Yafang; Xue, Fei; Zhou, Chun

doi:10.2147/copd.s266021

Cited by 13 publications

(9 citation statements)

References 48 publications

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“…As expected, TNF-α treatment increased the mRNA and protein level of MUC5AC in 16HBE cells 1D,E). The previous studies have demonstrated that miR-134-5p (miR-134) and miR-146a-5p (miR-146a) are involved in mucus hypersecretion in COPD (12,(16)(17)(18)(19). To explore the association of TNF-α-induced MUC5AC expression with these miRNAs, we first assessed whether TNF-α regulated the expression of these miRNAs.…”

Section: Tnf-α Treatment Increased Muc5ac Expression and Repressed Mir-134-5p (Mir-134) And Mir-146a-5p (Mir-146a) Expression In 16hbe Cementioning

confidence: 99%

“…Out of the 20 miRNAs, miR-134-5p and miR-146a-5p are lower expressed in COPD patients, and are crucial miRNAs associated with several mucus hypersecretion-correlated potential targets (12). miR-134-5p is a potential marker for the clinical diagnose of acute exacerbation of COPD (16). Down-regulated miR-146a-5p is involved in the pathogenesis of COPD through inducing more proinflammatory phenotype (17)(18)(19).…”

Section: Introductionmentioning

confidence: 99%

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Tumor necrosis factor-α promotes airway mucus hypersecretion by repressing miR-146a-5p and miR-134-5p levels in human airway epithelial cells

Fu¹,

Zhang²,

Zhang³

et al. 2021

Transl Cancer Res TCR

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Background: Airway mucus acts as an indispensable protective component of innate immune response against invading pathogens. However, airway mucus hypersecretion, largely consisting of mucin 5AC (MUC5AC), is the leading cause of airflow obstruction and airway hyperresponsiveness that contributes to chronic obstructive pulmonary disease (COPD). MicroRNAs (miRNAs) are frequently dysregulated in the pathogenesis of COPD, but the definite role of miRNAs in airway mucus hypersecretion is not well understood.Methods: A cell model of mucus hypersecretion was established in 16HBE cells by treatment with TNF-α.Cell viability and apoptosis were assessed using cell counting kit-8 (CCK-8) and flow cytometry, respectively.The aberrant expression of miR-146a-5p and miR-134-5p was assayed in TNF-α-treated 16HBE cells, and the effect of miR-146a-5p and miR-134-5p on regulating MUC5AC expression was evaluated using quantitative real-time PCR (qPCR) and Western blot analysis.Results: TNF-α treatment resulted in a significant decrease of cell viability, and increase of cell apoptosis and MUC5AC expression in 16HBE cells. Additionally, the expression of miR-134-5p and miR-146a-5p was markedly decreased in the cell model. Importantly, forced expression of miR-134-5p and miR-146a-5p significantly repressed TNF-α-induced upregulation of MUC5AC. Mechanistically, although miR-134-5p did not affect 16HBE cells viability and apoptosis, miR-134-5p partially blocked TNF-α-induced MUC5AC expression by inhibiting the activation of NF-κB signaling. On the other hand, miR-146a-5p enhanced cell viability and reduced cell apoptosis. miR-146a-5p also repressed TNF-α-induced MUC5AC expression by inhibiting p38 MAPK (mitogen-activated protein kinase) signaling activation. Conclusions:The current data demonstrated that both miR-134-5p and miR-146a-5p conferred protection against TNF-α-induced mucus hypersecretion through repressing NF-κB and p38 MAPK signaling, indicating that miR-134-5p and miR-146a-5p may serve as the biomarker for COPD.

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Section: Tnf-α Treatment Increased Muc5ac Expression and Repressed Mir-134-5p (Mir-134) And Mir-146a-5p (Mir-146a) Expression In 16hbe Cementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tumor necrosis factor-α promotes airway mucus hypersecretion by repressing miR-146a-5p and miR-134-5p levels in human airway epithelial cells

Fu¹,

Zhang²,

Zhang³

et al. 2021

Transl Cancer Res TCR

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“…D-dimer is a classic biomarker for detecting and evaluating PE progression. Of interest, the areas under the curves (AUCs) for the correlation of miR-134 and miR-1233 with PE progression were 0.931 (95% confidence interval [CI] 0.863–0.999) and 0.884 (95% CI 0.79–0.978), respectively, which were higher than those of D-dimer (0.628) (95% CI 0.447–0.809) and Wells score (0.577) (95% CI 0.389–0.765) ( Peng et al, 2020 ). This study provided evidence that the levels of serum miR-1233 and miR-134 are significantly upregulated in COPD patients with APE complication.…”

Section: Mirnas As Pe Biomarkersmentioning

confidence: 98%

The Function of microRNAs in Pulmonary Embolism: Review and Research Outlook

Luo

Shu

et al. 2021

Front. Pharmacol.

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Pulmonary embolism (PE) is a common pathologic condition that frequently occurs in patients with deep venous thrombosis. Severe PE may critically suppress cardiopulmonary function, thereby threatening the life of patients. Chronic pulmonary hypertension caused by PE may lead to deterioration of respiratory dysfunction, resulting in complete disability. MicroRNAs (miRNAs) are a group of abundantly expressed non-coding RNAs that exert multiple functions in regulating the transcriptome via post-transcriptional targeting of mRNAs. Specifically, miRNAs bind to target mRNAs in a matching mechanism between the miRNA seed sequence and mRNA 3ʹ UTR, thus modulating the transcript stability or subsequent translation activity by RNA-induced silencing complex. Current studies have reported the function of miRNAs as biomarkers of PE, revealing their mechanism, function, and targetome in venous thrombophilia. This review summarizes the literature on miRNA functions and downstream mechanisms in PE. We conclude that various related miRNAs play important roles in PE and have great potential as treatment targets. For clinical application, we propose that miRNA biomarkers combined with traditional biomarkers or miRNA signatures generated from microchips may serve as a great predictive tool for PE occurrence and prognosis. Further, therapies targeting miRNAs or their upstream/downstream molecules need to be developed more quickly to keep up with the progress of routine treatments, such as anticoagulation, thrombolysis, or surgery.

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“…59 Latest data identified circulating miR-1233 as a sensitive and specific biomarker for the early diagnosis of APE. 60 The above-mentioned differential expression experiments of miRNAs by quantitative reverse transcription polymerase chain reaction (qRT-PCR) indicate that miRNAs can be successfully used in the diagnosis of PE.…”

Section: Micrornasmentioning

confidence: 99%

Research progress on biomarkers of pulmonary embolism

Yang

Zhang

et al. 2021

Clinical Respiratory J

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Objectives: To present a review on the traditional and new biomarkers of pulmonary embolism (PE). Data source: A systematic search has been carried out using keywords as PE, biomarker, diagnosis and risk stratification. Results: The results of this work have been structured into three parts: first, conventional biomarkers for vascular, cardiac and inflammation, including static markers and dynamic markers for measuring the time course; next, a review of new biomarkers in recent years, such as RNAs and markers obtained through proteomics and mass spectrometry; finally, use of new detection methods to directly detect the activity of existing markers, such as the determination of coagulation factor II and plasmin activities based on the proteolytic activation of an engineered zymogen. Conclusions: This work summarized the characteristics of current traditional biomarkers for clinical diagnosis and risk stratification of PE, as well as a series of newly discovered biomarkers obtained through various clinical experimental methods.

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<p>The Predictive Value of microRNA-134 and microRNA-1233 for the Early Diagnosis of Acute Exacerbation of Chronic Obstructive Pulmonary Disease with Acute Pulmonary Embolism</p>

Cited by 13 publications

References 48 publications

Tumor necrosis factor-α promotes airway mucus hypersecretion by repressing miR-146a-5p and miR-134-5p levels in human airway epithelial cells

Tumor necrosis factor-α promotes airway mucus hypersecretion by repressing miR-146a-5p and miR-134-5p levels in human airway epithelial cells

The Function of microRNAs in Pulmonary Embolism: Review and Research Outlook

Research progress on biomarkers of pulmonary embolism

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