&lt;p&gt;The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle&lt;/p&gt;

Feuerstein, Giora; Mansfield, Michael A; Lelkes, Peter I.; Alesci, Salvatore; Marcinkiewicz, Cezary; Butlin, Nathaniel G.; Sternberg, Mark

doi:10.2147/ijn.s261952

Cited by 10 publications

(15 citation statements)

References 38 publications

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“…We have developed an innovative magnetic lock-in fluorescence method for absolute and ultrasensitive quantification of FNDs on a surface and in a solution. The method is highly selective, serving well as a rapid, quantitative, and sensitive platform for lateral flow immunoassays of infectious diseases using FNDs as reporters. , No extraction or other laborious separation steps prior to the fluorescence intensity measurement of FNDs in a complex biological medium are required. The simplicity of this magneto-optical method, together with the chemical robustness and biological inertness of the nanoprobes, makes it possible to study the pharmacokinetics and pharmacodynamics of FND-labeled cells in vivo. ,, Additionally, the method is applicable to monitoring tumor metastasis in small animal models after xenografting of FND-labeled cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…As shown in Figure 4c, we were able to achieve an LOD of ∼0.01 ng/mL, which is lower than the standard colloidal gold LFIA by ∼2 orders of magnitude based on color visualization. The method is expected to facilitate also the detection of viruses such as Ebola viruses as reported by Feuerstein et al 34 with FNDs as the reporters in their LFIA but without MMF.…”

Section: ■ Introductionmentioning

confidence: 94%

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Magnetically Modulated Fluorescence of Nitrogen-Vacancy Centers in Nanodiamonds for Ultrasensitive Biomedical Analysis

et al. 2021

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The negatively charged nitrogen-vacancy center in fluorescent nanodiamonds (FNDs) is a point defect with unique magneto-optical properties. It emits far-red fluorescence at ∼700 nm, and its intensity can be magnetically modulated with a depth of more than 10% at a field strength of 30 mT. We have closely examined this property and illustrated its practical use in biomedicine by applying a periodic, time-varying magnetic field to FNDs deposited on a surface or dispersed in a solution with a lock-in detection method. We achieved selective and sensitive detection of 100 nm FNDs on a nitrocellulose membrane at a particle density of 0.04 ng/mm 2 (or ∼2 × 10 4 particles/mm 2 ) and in an aqueous solution with a particle concentration of 1 ng/mL (or ∼1 fM) in 10 s as the detection limits. The utility and versatility of the technique were demonstrated with an application to background-free detection of FNDs as reporters for FND-based lateral flow immunoassays as well as selective quantification of FNDs in tissue digests for in vivo studies.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 94%

Magnetically Modulated Fluorescence of Nitrogen-Vacancy Centers in Nanodiamonds for Ultrasensitive Biomedical Analysis

et al. 2021

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“…The mAb capture/detection pairs used here should be readily adaptable for use in LFAs. Ideally, the 2E8 mAb would be used in a multiplexed assay in parallel with a mAb or ACE2 reagents capable of binding all variants [49][50][51]. Such tests can be an important adjunct to NAATs, as they are ideal for POC testing to protect vulnerable populations and broaden epidemiological surveillance.…”

Section: Discussionmentioning

confidence: 99%

A Strategy to Detect Emerging Non-Delta SARS-CoV-2 Variants with a Monoclonal Antibody Specific for the N501 Spike Residue

et al. 2021

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Efforts to control SARS-CoV-2 have been challenged by the emergence of variant strains that have important implications for clinical and epidemiological decision making. Four variants of concern (VOCs) have been designated by the Centers for Disease Control and Prevention (CDC), namely, B.1.617.2 (delta), B.1.1.7 (alpha), B.1.351 (beta), and P.1 (gamma), although the last three have been downgraded to variants being monitored (VBMs). VOCs and VBMs have shown increased transmissibility and/or disease severity, resistance to convalescent SARS-CoV-2 immunity and antibody therapeutics, and the potential to evade diagnostic detection. Methods are needed for point-of-care (POC) testing to rapidly identify these variants, protect vulnerable populations, and improve surveillance. Antigen-detection rapid diagnostic tests (Ag-RDTs) are ideal for POC use, but Ag-RDTs that recognize specific variants have not yet been implemented. Here, we describe a mAb (2E8) that is specific for the SARS-CoV-2 spike protein N501 residue. The 2E8 mAb can distinguish the delta VOC from variants with the N501Y meta-signature, which is characterized by convergent mutations that contribute to increased virulence and evasion of host immunity. Among the N501Y-containing mutants formerly designated as VOCs (alpha, beta, and gamma), a previously described mAb, CB6, can distinguish beta from alpha and gamma. When used in a sandwich ELISA, these mAbs sort these important SARS-CoV-2 variants into three diagnostic categories, namely, (1) delta, (2) alpha or gamma, and (3) beta. As delta is currently the predominant variant globally, they will be useful for POC testing to identify N501Y meta-signature variants, protect individuals in high-risk settings, and help detect epidemiological shifts among SARS-CoV-2 variants.

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“…In a study, Feuerstein et al used different sizes of FNDs (200, 400, and 800 nm) to develop a lateral flow technology for the detection of Ebola virus [ 120 ]. FND was conjugated with anti-Ebola virus glycoprotein monoclonal antibodies for a sandwich-type lateral flow test.…”

Section: Enhancement Of Sensitivity and Specificitymentioning

confidence: 99%

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19

Hsiao

Pham

et al. 2021

Biosensors

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The development of reliable and robust diagnostic tests is one of the most efficient methods to limit the spread of coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, most laboratory diagnostics for COVID-19, such as enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR), are expensive, time-consuming, and require highly trained professional operators. On the other hand, the lateral flow immunoassay (LFIA) is a simpler, cheaper device that can be operated by unskilled personnel easily. Unfortunately, the current technique has some limitations, mainly inaccuracy in detection. This review article aims to highlight recent advances in novel lateral flow technologies for detecting SARS-CoV-2 as well as innovative approaches to achieve highly sensitive and specific point-of-care testing. Lastly, we discuss future perspectives on how smartphones and Artificial Intelligence (AI) can be integrated to revolutionize disease detection as well as disease control and surveillance.

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<p>The Use of Near-Infrared Light-Emitting Fluorescent Nanodiamond Particles to Detect Ebola Virus Glycoprotein: Technology Development and Proof of Principle</p>

Cited by 10 publications

References 38 publications

Magnetically Modulated Fluorescence of Nitrogen-Vacancy Centers in Nanodiamonds for Ultrasensitive Biomedical Analysis

Magnetically Modulated Fluorescence of Nitrogen-Vacancy Centers in Nanodiamonds for Ultrasensitive Biomedical Analysis

A Strategy to Detect Emerging Non-Delta SARS-CoV-2 Variants with a Monoclonal Antibody Specific for the N501 Spike Residue

Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19

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