<span><strong>APPLICATION OF COMMERCIAL WHEY PROTEINS FOR EXTRACTION OF BIOACTIVE COMPOUNDS FROM ELDERBERRY</strong></span>

Abstract

ABSTRACT:Recently, interest in food of plant origin and its pro-health properties have increased. Plant products due to its significant antioxidant activity should be a constant part of the human diet. Elderberry is a The aim of this work was to extract bioactive components from elderberry (Sambucus nigra) fruits and flowers by means of various extraction solutions. The extraction solution were WPC (whey protein concentrate) and WPI (whey protein isolate) solution. Comparative extracts were also made using ethanol solutions and distilled water as control. Obtained extracts were analyzed by determination of: total reduction capacity using Folin-Ciocalteu reagent, flavonoid content, antioxidant activity using DPPH reagent. In WPC and WPI protein extracts, the extraction of bioactive components is more efficient than in the case of water extraction. This could be seen at the lowest concentration of both WPI and WPC.