The effects of changes in linewidth, barrier type and anneal temperature on electromigration (EM) reliability of inlaid Cu interconnect lines were investigated. Methods developed for quantifying changes in grain size and orientation with changes in processing are detailed and applied to understand their impact on electromigration. While interfaces and microstructure both play a role in Cu reliability, interface diffusion is the dominant effect. For a constant interface and linewidth, grain size is shown to affect reliability.