&lt;title&gt;Microtools for cell handling&lt;/title&gt;

Telleman, Pieter; Larsen, Ulrik; Kutter, Jörg Peter; Friis, Peter; Wolff, Anders

doi:10.1117/12.395646

Cited by 5 publications

(3 citation statements)

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“…These approaches are disadvantageous, especially for valuable or rare samples. It has been widely acknowledged that many microfluidic devices had great potential for single-cell experiments (Telleman et al 2000;Muller et al 2003). Over the years, many methods have been developed, including optical tweezers (Grover et al 2001), optoelectronic tweezers (Ohta et al 2004), micro fluorescence-activated cell sorters (lFACS) (Bonner et al 1972;Fu et al 1999), magnetic-activated cell separation (lMACS) (Telleman et al 1998), microfabricated flow switches (Larsen et al 1996;Lee et al 2001;Huh et al 2001;Tashoro et al 2000), and electrical-fieldbased manipulations and separations (Ichiki et al 2001;Takahashi et al 2004;Schnelle et al 1993;Fuhr et al 1994;Kaler et al 1992;Voldman et al 2003;Pethig 1996;Becker et al 1995;Morgan et al 1999;Hughes 2003;Mü ller et al 1999;Jones and Washizu 1994;Schnelle et al 1999;Fiedler 1998;Dü rr et al 2003;Reichle et al 1999;Schnelle et al 2000;Pohl 1978).…”

Section: Introductionmentioning

confidence: 99%

Studies of particle holding, separating, and focusing using convergent electrodes in microsorters

Leu

Chen

Hsiao

2005

Microfluid Nanofluid

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This paper presents numerical simulation results on the efficacy of dielectrophoretic (DEP) convergent electrodes in a particle sorter. DEP forces created by non-uniform electric fields are used as holding forces to trap and select the particles from a mixture of many samples, as well as confining forces to focus the particles into a single particle stream in the microsorter for further analysis. The key mechanism of the sorter that can hold particles against destabilizing fluid flows is investigated in this study. A barrier is found at X/L=0.84 and Y=0 in the present DEP sorter. By comparing the DEP and hydrodynamic forces at the barrier, one can determine the release velocity when the zero-net-force condition ceases to exist and the particles start to be released.

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Section: Introductionmentioning

confidence: 99%

Studies of particle holding, separating, and focusing using convergent electrodes in microsorters

Leu

Chen

Hsiao

2005

Microfluid Nanofluid

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“…It has been widely acknowledged that the effective sorting in many microfluidic and lab-on-a-chip devices provide many advantages over the conventional fluorescent activated cell sorting ͑FACS͒, 1 such as low consumption of samples without sacrificing sensitivity, closed system reducing the potential biohazard risks and preventing cross-contamination, and feasibility of making portable and disposable devices. 2 Over the years, scientists have developed many particle and cell handling microfluidic devices-for example, microfabricated fluorescenceactivated cell sorting ͑FACS͒, 3,4 magnetic-activated cell separation ͑MACS͒, 4 automated single-cell sorting using dual-beam optical trapping, 5 optoelectronic tweezers for particle manipulation, 6 and microfabricated flow switches for sample injection and cell/particle sorting. 7,8 Air-liquid two-phase 9 and three-dimensional ͑3-D͒ sheath flow-type 10 microchannels are also reported.…”

Section: Introductionmentioning

confidence: 99%

Studies of particle levitation in a dielectrophoretic field-flow fraction–based microsorter

Leu

Weng

2009

J. Micro/Nanolith. MEMS MOEMS

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We study particle levitation in a dielectrophoretic field-flow fraction ͑DEP-FFF͒ flow sorter by using theoretical and numerical methods. By balancing DEP forces with gravitational and buoyant forces, one can obtain the analytical solution for the particle levitation height. Numerical simulation is carried out and used to compare with the analytical prediction. One can find that there exists a maximum particle levitation height at a specific electrode width ͑d͒ for each applied voltage. The maximum levitation height happens at h p / d = 0.95. The particle behaviors can be discussed based on the ratio between levitation height ͑h p ͒ and the width of electrode ͑d͒. When levitation height is higher than h p / d Ͼ 0.6, simulation results show excellent agreement ͑less than 2% error͒ with the first-order approximated analytical solution. When levitation height is between 0.43Ͻ h p / d Ͻ 0.6, the results start to show the large discrepancies ͑more than 2% error͒ between simulation and the firstorder approximated analytical solution. A higher order theoretical solution has to be considered for this situation. When levitation height is h p / d Ͻ 0.43, particles will stick on the bottom wall. Approximate theoretical solution is no longer applicable.

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“…It has been widely acknowledged that the effective sorting in many micro-fluidic and Lab-On-A-Chip devices provided many advantages over the conventional fluorescent activated cell sorting (FACS) [1], such as low consumption of samples without sacrificing sensitivity, closed system reducing the potential biohazard risks and preventing cross-contamination, and feasibility of making portable and disposable devices [2]. Over the years, the scientists have developed many particle and cell handling microfluidic devices.…”

Section: Introductionmentioning

confidence: 99%

Design and Simulation of Continuous Dielectrophoretic Flow Sorters

2006

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This paper was an attempt to investigate, through numerical simulation, the designs of DEP flow sorters when applied with different ratios of the electrodes to generate different electrical fields, and to explore the sorting capability of the flow sorters, defined as the degree of particle deflection, under different operation of parameters.In order to obtain the maximal DEP negative particle deflection, which was believed as an indicator of greater sorting capability, we have investigated different non-uniform electrical fields produced by combinations of electrodes with different length of two poles, ranging from 1:2 up to 1:9. The finding of numerical simulation indicated that the length ratio 1:3 of the electrode poles produced the electrical fields that maximized the particle deflection.Moreover, different parameters of applied voltage, flow rate, particle diameters, and distance between two electrical poles were designed to investigate their effects on particle deflection of flow sorters. The numerical simulation of the study showed that the DEP flow sorter was demonstrated as a linear system with respect to the applied voltage and particle diameter. In this study, we tried to operationally define flow rate as the time duration while the flow passed the electrical fields, and thus investigated how particle deflect with the different time given. We found that the particle deflected more when the flow was allowed with longer time to pass the electrical fields. The study also showed that the distance the particles deflect from the centerline is in inverse proportion to the square distance between the two electrical poles.

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<title>Microtools for cell handling</title>

Cited by 5 publications

References 0 publications

Studies of particle holding, separating, and focusing using convergent electrodes in microsorters

Studies of particle holding, separating, and focusing using convergent electrodes in microsorters

Studies of particle levitation in a dielectrophoretic field-flow fraction–based microsorter

Design and Simulation of Continuous Dielectrophoretic Flow Sorters

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