&lt;title&gt;Physico-chemical survival pattern for the radiophile D. radiodurans: a polyextremophile model for life on Mars&lt;/title&gt;

Richmond, Robert C.; Sridhar, R.; Zhou, Y.; Daly, Michael J.

doi:10.1117/12.375078

Cited by 25 publications

(30 citation statements)

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“…In contrast to data from previous reports showing that frozen conditions do not offer much protection against radiation damage in D. radiodurans (166), more recent data show that D. radiodurans cells irradiated on dry ice (Ϫ70°C) have a higher gamma ray survival rate than do cells irradiated on ice or at room temperature (128,130,519). The increase in radiation resistance under frozen irradiation conditions may be due to the diminished oxygen effects on proteins (120,122).…”

Section: Ionizing Radiation Resistance Of D Radioduranscontrasting

confidence: 53%

“…Alternatively, the extreme radiation resistance may have evolved as an adaptation to permafrost or semifrozen conditions where background radiation-induced DNA damage is accumulated (519) or to high natural ionizing radiation levels in deeply buried manganese-rich marine sediments ("radiation adaptation hypothesis") (555). D'Hondt et al (140b) found microbial activities in these deeply buried sediments with high ionizing radiation levels.…”

Section: Ionizing Radiation Resistance Of D Radioduransmentioning

confidence: 99%

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Oxidative Stress Resistance inDeinococcus radiodurans

Slade

Radman

2011

Microbiol Mol Biol Rev

660

639

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SUMMARY Deinococcus radiodurans is a robust bacterium best known for its capacity to repair massive DNA damage efficiently and accurately. It is extremely resistant to many DNA-damaging agents, including ionizing radiation and UV radiation (100 to 295 nm), desiccation, and mitomycin C, which induce oxidative damage not only to DNA but also to all cellular macromolecules via the production of reactive oxygen species. The extreme resilience of D. radiodurans to oxidative stress is imparted synergistically by an efficient protection of proteins against oxidative stress and an efficient DNA repair mechanism, enhanced by functional redundancies in both systems. D. radiodurans assets for the prevention of and recovery from oxidative stress are extensively reviewed here. Radiation- and desiccation-resistant bacteria such as D. radiodurans have substantially lower protein oxidation levels than do sensitive bacteria but have similar yields of DNA double-strand breaks. These findings challenge the concept of DNA as the primary target of radiation toxicity while advancing protein damage, and the protection of proteins against oxidative damage, as a new paradigm of radiation toxicity and survival. The protection of DNA repair and other proteins against oxidative damage is imparted by enzymatic and nonenzymatic antioxidant defense systems dominated by divalent manganese complexes. Given that oxidative stress caused by the accumulation of reactive oxygen species is associated with aging and cancer, a comprehensive outlook on D. radiodurans strategies of combating oxidative stress may open new avenues for antiaging and anticancer treatments. The study of the antioxidation protection in D. radiodurans is therefore of considerable potential interest for medicine and public health.

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Section: Ionizing Radiation Resistance Of D Radioduranscontrasting

confidence: 53%

Section: Ionizing Radiation Resistance Of D Radioduransmentioning

confidence: 99%

Oxidative Stress Resistance inDeinococcus radiodurans

Slade

Radman

2011

Microbiol Mol Biol Rev

660

639

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“…As suggested above, it is possible that their extreme proficiency at DNA repair is related to the selective advantage in environments where they are prone to damage during long periods of desiccation (135). More recently, it has been proposed that adaptation could also occur in permafrost or other semifrozen conditions where cryptobiotic microbes with extremely long generation times could be selected with metabolic processes able to repair the unavoidable accumulation of background radiation-induced DNA damage (171).…”

Section: Isolationmentioning

confidence: 99%

Genome of the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans Viewed from the Perspective of Comparative Genomics

Makarova

Aravind

Wolf

et al. 2001

Microbiol Mol Biol Rev

Self Cite

656

612

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The bacterium Deinococcus radiodurans shows remarkable resistance to a range of damage caused by ionizing radiation, desiccation, UV radiation, oxidizing agents, and electrophilic mutagens. D. radiodurans is best known for its extreme resistance to ionizing radiation; not only can it grow continuously in the presence of chronic radiation (6 kilorads/h), but also it can survive acute exposures to gamma radiation exceeding 1,500 kilorads without dying or undergoing induced mutation. These characteristics were the impetus for sequencing the genome of D. radiodurans and the ongoing development of its use for bioremediation of radioactive wastes. Although it is known that these multiple resistance phenotypes stem from efficient DNA repair processes, the mechanisms underlying these extraordinary repair capabilities remain poorly understood. In this work we present an extensive comparative sequence analysis of the Deinococcus genome. Deinococcus is the first representative with a completely sequenced genome from a distinct bacterial lineage of extremophiles, the Thermus-Deinococcus group. Phylogenetic tree analysis, combined with the identification of several synapomorphies between Thermus and Deinococcus, supports the hypothesis that it is an ancient group with no clear affinities to any of the other known bacterial lineages. Distinctive features of the Deinococcus genome as well as features shared with other free-living bacteria were revealed by comparison of its proteome to the collection of clusters of orthologous groups of proteins. Analysis of paralogs in Deinococcus has revealed several unique protein families. In addition, specific expansions of several other families including phosphatases, proteases, acyltransferases, and Nudix family pyrophosphohydrolases were detected. Genes that potentially affect DNA repair and recombination and stress responses were investigated in detail. Some proteins appear to have been horizontally transferred from eukaryotes and are not present in other bacteria. For example, three proteins homologous to plant desiccation resistance proteins were identified, and these are particularly interesting because of the correlation between desiccation and radiation resistance. Compared to other bacteria, the D. radiodurans genome is enriched in repetitive sequences, namely, IS-like transposons and small intergenic repeats. In combination, these observations suggest that several different biological mechanisms contribute to the multiple DNA repair-dependent phenotypes of this organism

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“…radiodurans is a polyextremophile (48), showing remarkable resistance to a range of severe damage caused by ionizing radiation, desiccation, UV radiation, oxidizing agents, or electrophilic mutagens (40). This bacterium is most famous for its resistance to ionizing radiation.…”

mentioning

confidence: 99%