&lt;title&gt;Sensitization and photodynamic therapy of esophageal,duodenal, and colonic tumors with 5-aminolaevulinic acid (ALA) induced protoporphyrin IX (PPIX)&lt;/title&gt;

Mlkvý, Peter; Messmann, Helmut; Reguła, Jarosław; Conio, Mássimo; Pauer, M; Millson, Charles; MacRobert, Alexander J.; Bown, Stephen G.

doi:10.1117/12.203379

Cited by 24 publications

(30 citation statements)

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“…This finding has clinical relevance since the ALA dosage (50 mg/kg) utilized the peritoneal cavity. In conclusion, specific detection of rat ovarian cancer could be achieved with administration of a in this study has already been shown to be safe in human clinial trials [17][18][19].…”

Section: Discussionmentioning

confidence: 92%

In VivoFluorescence Detection of Ovarian Cancer in the NuTu-19 Epithelial Ovarian Cancer Animal Model Using 5-Aminolevulinic Acid (ALA)

Major¹,

Rose²,

Chapman³

et al. 1997

Gynecologic Oncology

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result, it is necessary to develop improved diagnostic andThe purpose of this study was to determine whether in vivo therapeutic methods which offer the possibility of early defluorescence detection of protoporphyrin IX (PpIX) could be used tection and more effective eradication of the disease. The use to identify intraperitoneal micrometastases of epithelial ovarian of photomedicine techniques such as photodynamic therapy carcinoma after application of 5-aminolevulinic acid (ALA). ALA (PDT) and in vivo fluorescence detection using lesion lowas applied intraperitoneal at different concentrations (25, 50, calizing photosensitizers have been suggested as novel interand 100 mg/kg) and iv (100 mg/kg) to immunocompetent Fischer ventions in cancer patients [3,4]. rats bearing a syngeneic epithelial ovarian carcinoma. At dif-While there has been great interest in the use of PDT in ferent time intervals after ALA administration (1.5, 3, and 6 hr) the peritoneal cavity was illuminated with ultraviolet (uv) light. cancer treatment, there have been no reports of its use as In vivo fluorescence of PpIX initially was determined by direct a diagnostic aid in ovarian cancer second-look laparotomy visualization. Subsequently ex vivo measurements were made with procedures. Second-look procedures have not been shown a slow-scan, thermoelectrically cooled CCD camera. Red in vivo to have a positive effect on patient survival and are currently fluorescence was observed in ovarian micrometastases smaller than only recommended for patients on research protocols or in 0.5 mm in 100% of the ALA-administered animals independent settings where salvage therapies are available [5,6]. In addiof time interval, drug concentration, or route of administration.

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Section: Discussionmentioning

confidence: 92%

In VivoFluorescence Detection of Ovarian Cancer in the NuTu-19 Epithelial Ovarian Cancer Animal Model Using 5-Aminolevulinic Acid (ALA)

Major¹,

Rose²,

Chapman³

et al. 1997

Gynecologic Oncology

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“…The MPD for UVA is then defined as the lowest UVA dose producing an erythema and/or pigmentation immediately and 20 min after UVA exposure. In subjects with healthy skin the mean threshold dose of UVA is about 21 J/cm 2 [10]. The light provocation was carried out with an UVA source (UVA 700, Waldmann Medizintechnik, Villingen-Schwenningen, Germany; Ï cm = 320-400 nm).…”

Section: Methodsmentioning

confidence: 99%

“…The MPD for UVA of each patient was determined with the established and standardized progressively graded light test [10]. Briefly, defined areas (n = 9; area 1 cm 2 ) of untanned skin at the lower back of the patients were exposed to a series of increasing UVA doses (5-80 J/cm 2 ).…”

Section: Methodsmentioning

confidence: 99%

No Generalized Skin Phototoxicity after Intravesical Application of 5-Aminolevulinic Acid for Fluorescence Diagnosis ofSuperficial Bladder Cancer

et al. 2000

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Introduction: A prospective monocenter open study was carried out to evaluate if generalized phototoxic skin reactions occur after intravesical application of 5-aminolevulinic acid (ALA) for fluorescence diagnosis of superficial bladder carcinomas. Patients and Methods: On 21 patients, skin phototoxicity was determined prior to as well as 4, 8 and 28 h after intravesical instillation of a 3% ALA solution by exposing small skin areas to a progressively graded series of defined UVA-light doses (n = 9; 5–80 J/cm²). Results: Prior to ALA instillation, erythema or pigmentation as signs of cutaneous phototoxicity occurred at an UVA-light dose of 28 ± 1.5 J/cm² (mean ± SEM). A reduction of the minimal phototoxic dose (MPD) was not detected 4 (28 ± 1.9 J/cm²), 8 (28 ± 1.6 J/cm²) and 28 h (28 ± 1.5 J/cm²) after ALA instillation. Consequently phototoxic reactions were not observed. Conclusions: A reduction of MPD for UVA was not detected. Therefore, it is not necessary to protect the skin of patients from ambient or daylight after intravesical instillation of ALA for fluorescence diagnosis.

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“…Although PpIX biosynthesis is an active pathway in all nucleated cells, preferential formation/ accumulation of PpIX has been demonstrated in malignant tissues (Batlle, 1993;Kennedy and Pottier, 1994). ALA-PDT has been used previously on a variety of normal and malignant cell types in vitro (Malik and Lugaci, 1987;linuma et al, 1994) (Kennedy and Pottier, 1994;Cairnduff et al, 1994;Gossner et al, 1995;Lang et al, 1995;Lui et al, 1995;Mlkvy et al, 1995;Regula et al, 1995).…”

Section: -Aminolaevulinic Acidmentioning

confidence: 99%

In vitro studies on the potential use of 5-aminolaevulinic acid-mediated photodynamic therapy for gynaecological tumours

Rossi

Campbell²,

Pottier³

et al. 1996

Br J Cancer

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Summary Results are reported on the sensitivity of various gynaecological tumour cell lines to 5-aminolaevulinic acid-induced protoporphyrin IX-sensitised photodynamic therapy (ALA-PDT) in vitro. All cell lines tested accumulated ALA-induced protoporphyrin IX (PpIX) and demonstrated good sensitivity to ALA-PDT. Localisation of PpIX in the mitochondria was demonstrated by fluorescence microscopy. Subcellular damage following ALA-PDT was observed using transmission electron microscopy. This damage was localised initially to the mitochondria, with damage to membranes and the nucleus and complete loss of intracytoplasmic organisation being observed subsequently. There was no apparent difference in ALA-PDT response between a multidrug-resistant ovarian carcinoma cell line and its parent line. These results indicate that ALA-PDT has potential for application to therapy of gynaecological malignancies.

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<title>Sensitization and photodynamic therapy of esophageal,duodenal, and colonic tumors with 5-aminolaevulinic acid (ALA) induced protoporphyrin IX (PPIX)</title>

Cited by 24 publications

References 0 publications

In VivoFluorescence Detection of Ovarian Cancer in the NuTu-19 Epithelial Ovarian Cancer Animal Model Using 5-Aminolevulinic Acid (ALA)

In VivoFluorescence Detection of Ovarian Cancer in the NuTu-19 Epithelial Ovarian Cancer Animal Model Using 5-Aminolevulinic Acid (ALA)

No Generalized Skin Phototoxicity after Intravesical Application of 5-Aminolevulinic Acid for Fluorescence Diagnosis ofSuperficial Bladder Cancer

In vitro studies on the potential use of 5-aminolaevulinic acid-mediated photodynamic therapy for gynaecological tumours

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