&lt;title&gt;Stimulation of chondrocyte proliferation following photothermal, thermal, and mechanical injury in ex-vivo cartilage grafts&lt;/title&gt;

Pandoh, Nidhi S.; Truong, Mai Thy; Diaz-Valdes, Sergio H.; Gardiner, David M.; Wong, Brian J. F.

doi:10.1117/12.472537

Cited by 2 publications

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“…Since the 1980, lasers have been used to produce these thermal interactions in cartilage. Laser stimulated growth of chondrocytes is a welldocumented phenomenon marked by dividing chondrocytes on the peripheries of laser irradiated areas in cartilage [1][2][3][4][5][6]. Our group has studied this phenomenon in rabbit nasal septal cartilage using an Nd:YAG laser that generates infrared light that is poorly absorbed by cartilage.…”

Section: Introductionmentioning

confidence: 99%

“…One method used to identify proliferating chondrocytes in laser-irradiated cartilage is a whole mount double antibody staining system [4][5][6][7]. Laser irradiated rabbit nasal septal cartilage tissue samples were placed in culture media containing BrdU, a brominated analog of thymidine that is selectively incorporated into cell DNA during S-phase of the cell cycle.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Nd:YAG laser-induced injury to rabbit nasal septal cartilage using confocal microscopy and Live/Dead assay in an ex vivo model

Krasieva

Zorin

et al. 2004

SPIE Proceedings

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Identification of proliferating chondrocytes along the periphery of laser ablation sites in irradiated cartilage has led to interest in studying the use of laser heating alone to stimulate chondrocyte growth. However, excessive heat produced by a laser can also cause chondrocyte necrosis and apoptosis. The objective of this study was to evaluate acute injury to cartilage following irradiation by an Nd:YAG (λ=1.32µm) laser in intact ex-vivo tissue specimens. Rabbit nasal septal cartilage was irradiated using an Nd:YAG laser using pulse durations (4, 6, and 8 seconds) and power (4,6, and 8 watts) settings previously determined to produce cell division. Immediately after laser irradiation, the extent of thermal injury to the cartilage samples was evaluated using a Live/Dead® cell viability assay combined with confocal microscopy. Thermal injury was assessed with respect to distribution of live and dead cells surrounding the laser spot where regeneration was previously observed. The cell viability assay identified necrotic tissue within and immediately around the laser spot. Moving away from the center of the laser spot, a mixed population of necrotic and live chondrocytes was observed. As expected, a correlation between irradiation time, power and degree of injury was found. The results of this experiment will be used determine the threshold required to produce regeneration while minimizing thermal injury.

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