&lt;title&gt;Treatment of rat Walker-256 carcinosarcoma with photodynamic therapy and endotoxin irradiated with high-energy electrons&lt;/title&gt;

Dima, Vasile F.; Vasiliu, V.; Laky, D; Ionescu, M; Dima, S.

doi:10.1117/12.168706

Cited by 4 publications

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“…Experimental approaches and clinical investigations have shown that the association of two or more modalities for cancer treatment in animals and humans presents evident advantages and has become a frequently applied treatment (7,8) .…”

Section: Introductionmentioning

confidence: 99%

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A Modified Approach to the Adoptive Immunotherapy of Walker-256 Carcinosarcoma With a Combination of Activated Macrophages and Photodynamic Therapy

Vasiliu

Laky

et al. 2006

LASER THERAPY

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Experiments were performed on five batches of Wistar inbred rats with Walker-256 carcinoma receiving sole (PDT, MAK) or combined therapy (PDT+MAK-A; PDT+MAK-B); the control batch (HBSS) consisted of animals with untreated tumors. The results were as follows: (a) the sole treatment (PDT, MAK) gave survival rates between 37.7 and 47.5 %, the "combined" therapy in five doses increased significantly (70.8 %) the survival rate of tumor bearing rats as well as the rate of complete regression (82.1%). The cell-mediated immunity test and histopathological and as well as the electron microscopy observation were in full agreement with the results above. Summing up, this results demonstrate that "combined" photodynamic therapy with intra and peritumoral MAK infusion stimulated cell-mediated antitumoral activity, increased survival rates and reduces incidence of Walker-256 carcinoma in rat model.

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Section: Introductionmentioning

confidence: 99%

“…In many laboratories, it has been proved that activation of murine macrophages can induce regression of tumors (7) and metastases (8) . Similarly, human and murine macrophages differentiated from blood monocytes and activated with interferon have been used in adoptive immunotherapy (9,10) .…”

Section: Introductionmentioning

confidence: 99%

A Modified Approach to the Adoptive Immunotherapy of Walker-256 Carcinosarcoma With a Combination of Activated Macrophages and Photodynamic Therapy

Vasiliu

Laky

et al. 2006

LASER THERAPY

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Induction of Tumor Immunity by Photodynamic Therapy

Korbelik¹

1996

Journal of Clinical Laser Medicine & Surgery

230

144

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The mechanism of tumor destruction by photodynamic therapy (PDT) incorporates a variety of events leading to inactivation of tumor cells. The unique feature of PDT is the mobilization of the host to participate in the eradication of treated cancer. A critical element is the induced inflammation at the treated site associated with massive invasion of activated myeloid cells. In addition to further destruction of cancer cells, conditions are created for the presentation of tumor antigens with subsequent activation of lymphoid cells, leading to tumor-specific immunity. This inflammation-primed immune development process results in generation of tumor-specific immune memory cells that appear to be elicited against both strongly and poorly immunogenic PDT-treated cancers. Once generated by PDT, it is conceivable that these immune cells (especially if further expanded and activated by adjuvant immunotherapy) can be engaged in additional eradication of disseminated and/or metastatic lesions of the same cancer. A number of immunotherapy regimens have already been proven effective in enhancing the curative effect of PDT with various animal tumor models. Inflamed cancerous tissue at the PDT-treated site appears to exert powerful attracting signals for immune cells activated by different immunotherapy regimens.

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Role of the Immune System in Mediating the Antitumor Effect of Benzophenothiazine Photodynamic Therapy

Hendrzak-Henion

Knisely

Cincotta

et al. 1999

Photochem & Photobiology

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The role of the host immune system in contributing to tumor regression following benzophenothiazine photodynamic therapy (PDT) was examined. Photodynamic therapy with 2-iodo-5-ethylamino-9-diethylaminobenzo[a]-phenothiazinium chloride (2I-EtNBS) eradicated EMT-6 mammary fibrosarcomas in 75-100% of treated mice. In contrast, PDT failed to inhibit tumor growth in T-cell-deficient nude mice. Furthermore, T-cell depletion studies with anti-CD8 antibody revealed that the CD8+ T-cell population was critical for an effective PDT response (tumor volume 14 days post-PDT: 262 mm3 vs 59 mm3 in controls; P < 0.01). Because anti-CD4 antibody inhibited tumor growth in the absence of PDT, the role of CD4+ T cells remains unclear. Depletion of natural killer (NK) cells in vivo with anti-asialo-GM1 antibody significantly reduced a suboptimal PDT effect relative to vehicle controls (tumor volume 13 days post-PDT: 513 mm3 vs 85 mm3, respectively; P < 0.001). However, splenic NK cells obtained from PDT-treated tumor-bearing mice were not cytotoxic in vitro against EMT-6 cells, suggesting that NK cells contribute to the PDT effect in vivo by an indirect mechanism. In addition, when mice with complete tumor regression following PDT were rechallenged 28 days later with 5 x 10(5) EMT-6 cells, tumor growth was significantly inhibited as compared to controls (tumor volume 40 days postrechallenge: 137 mm3 vs 833 mm3 in controls; P < 0.03; percent animals without tumor in five experiments: 67% vs 8% in controls). Collectively, these results demonstrate that CD8+ T cells are required to prevent tumor regrowth after 2I-EtNBS-PDT, NK cells contribute to this response and such PDT can elicit protective antitumor immunity.

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<title>Treatment of rat Walker-256 carcinosarcoma with photodynamic therapy and endotoxin irradiated with high-energy electrons</title>

Cited by 4 publications

References 0 publications

A Modified Approach to the Adoptive Immunotherapy of Walker-256 Carcinosarcoma With a Combination of Activated Macrophages and Photodynamic Therapy

A Modified Approach to the Adoptive Immunotherapy of Walker-256 Carcinosarcoma With a Combination of Activated Macrophages and Photodynamic Therapy

Induction of Tumor Immunity by Photodynamic Therapy

Role of the Immune System in Mediating the Antitumor Effect of Benzophenothiazine Photodynamic Therapy

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