1972
DOI: 10.1002/jsfa.2740231003
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Lucerne tannins. II. Isolation of tannins from lucerne, their nature and influence on the digestive enzymes in vitro

Abstract: Tannic substances were isolated from dehydrated lucerne meal and it was found that they contained free gallic acid, gallotannins and flavanols. In the procedure for their isolation, cation and anion exchange resins, Amberlite IR-120 and Amberlite IR-45, were used. The isolated tannin was tested as an inhibitor of tryptic digestion of casein, L-amylolytic digestion of starch andlipase enzymes. In in vitro studies it was found that the isolated tannin has a high inhibitory action on digestive enzymes.

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Cited by 44 publications
(12 citation statements)
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“…Legumes in general have long been known for higher percentage of CP, Ca, and Mg than grasses. Forages also can contain antiquality factors such as saponin, tannin, and trypsin inhibitor (Walter et al, 1955;Millic et al, 1972;Jung and Fahey, 1983). Tall fescue is often infected with the endophyte fungus, Neotyphodium coenophialum (Glenn et al, 1996), resulting in several disorders in grazing ruminants and horses (Stuedemann and Hoveland, 1988).…”
Section: Start Of 4-h Periodsmentioning
confidence: 99%
“…Legumes in general have long been known for higher percentage of CP, Ca, and Mg than grasses. Forages also can contain antiquality factors such as saponin, tannin, and trypsin inhibitor (Walter et al, 1955;Millic et al, 1972;Jung and Fahey, 1983). Tall fescue is often infected with the endophyte fungus, Neotyphodium coenophialum (Glenn et al, 1996), resulting in several disorders in grazing ruminants and horses (Stuedemann and Hoveland, 1988).…”
Section: Start Of 4-h Periodsmentioning
confidence: 99%
“…et al. 1972, Griffiths 1979, Barry and Duncan 1984, have indicated Consequently, the best equation for prediction of dietary crude tannins have the capacity to bind with proteins to form complexes protein contains these 2 variables.…”
Section: Prediction Of Diet Qualitymentioning
confidence: 99%
“…One per cent tannin fed to chickens influenced weight gain and depressed feed conversion by 6 per cent [3]. Actually, tannins exhibit a high binding affinity for a number of enzymes and behave as enzyme inactivators [61]. The ability of tannins to lower protein digestibility is not the only factor explaining the adverse nutritional effects induced by them.…”
Section: Factors Affecting Protein Digestibilitymentioning
confidence: 99%