Lucifer dyes—highly fluorescent dyes for biological tracing

Stewart, Walter W.

doi:10.1038/292017a0

Cited by 494 publications

(175 citation statements)

References 39 publications

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“…1,8-Naphthalimides are generally fluorescent compounds for which a series of biological (local anesthetics [4], DNA cleaving agents [5], tumoricidals [6] and non-biological optical brighteners [7], lucifer dyes [8]) applications have been found. 1,8-Naphthalimide and bisnaphthalimide derivatives are promising anticancer agents [9,10], the sulfonated derivatives are good antiviral agents with selective in vitro activity against the human immunity deficiency virus, HIV-1 [11].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Photophysical Characterizations of Thermal -Stable Naphthalene Benzimidazoles

2011

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Section: Introductionmentioning

confidence: 99%

Synthesis and Photophysical Characterizations of Thermal -Stable Naphthalene Benzimidazoles

2011

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“…1, 2 Apart from their widespread use as tools for studying neuronal morphology 1,3-5 and function, 6,7 they have also served as cytoplasmic or endosomal markers, 8,9 reactive labels for tissue staining, 10,11 tracers in confocal or electron microscopy, 2,12-14 protein labelling agents, 15-17 and tracers of cell-cell fusion, membrane permeability, or cell uptake. 18-20 These dyes are based on a sulfonated 4-amino-1,8-naphthalimide moiety with a variable substituent on the imide nitrogen.…”

Section: Introductionmentioning

confidence: 99%

Excited-state dynamics of the fluorescent probe Lucifer Yellow in liquid solutions and in heterogeneous media

Fürstenberg

Vauthey

2005

Photochem Photobiol Sci

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The photophysics of the dye Lucifer Yellow ethylenediamine (LYen) has been investigated in various polar solvents. The main deactivation pathways of its first singlet excited state are the fluorescence and the intersystem crossing. In water, non-radiative decay by intermolecular proton transfer becomes a significant deactivation channel. The early fluorescence dynamics, which was investigated in liquids and in reverse micelles, was found to depend substantially on the environment. An important static quenching of LYen by tryptophan and indole occurring in the subpicosecond timescale was observed. The use of the fluorescence dynamics of LYen as a local probe is illustrated by preliminary results obtained with a biotinylated Lucifer Yellow derivative complexed with avidin.

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“…The fluorescent dye Lucifer Yellow (Steward, 1981) (Zar, 1974). Probability < 0 05 was accepted as significant.…”

Section: Electrical Behaviour Of Gastric Neuronesmentioning

confidence: 99%

Electrical behaviour of myenteric neurones in the gastric corpus of the guinea‐pig.

Schemann

Wood

1989

The Journal of Physiology

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SUMMARY1. Electrical behaviour of ganglion cells in the myenteric plexus of the guinea-pig stomach was investigated using intracellular recording methods.2. Three subpopulations were identified and classified for convenience of discussion as gastric I, II and III neurones. Gastric I neurones were characterized by repetitive spike discharge during depolarizing current pulses and by higher input resistance than the other types. Gastric II neurones discharged one or two spikes only at the onset of long-lasting depolarizing current pulses. Gastric III neurones did not discharge spikes to depolarizing current pulses and had higher membrane potentials and lower input resistances that the other types. Non-stimulus evoked discharge ('spontaneous' discharge) did not occur in any of the neurones.3. Resting membrane potentials were generated primarily by resting K+ conductance, but were smaller than the estimated K+ equilibrium potential. Analysis based on the constant field equation predicted lower K+ conductance in gastric I than in gastric III neurones. 4. Action potentials in gastric I and II neurones were suppressed or blocked by tetrodotoxin. Spikes that were broadened by tetraethylammonium appeared to have an inward component of Ca2+ current.5. Hyperpolarizing after-potentials were associated with the spikes of both kinds of neurones. These after-potentials had much shorter duration ( < 300 ms) than the post-spike hyperpolarization of AH/type 2 intestinal neurones and unlike intestinal neurones there was no latency between the positive after-potential of the spike and the onset of the hyperpolarization. After-hyperpolarization in the gastric neurones was enhanced when the spikes were broadened by tetraethylammonium and was suppressed by removal of Ca2+ from the bathing solution.6. Treatment with either tetraethylammonium or 4-aminopyridine enhanced excitability and induced 'spontaneously' occurring repetitive spike discharge.7. The electrophysiological behaviour of gastric myenteric neurones differed significantly from intestinal neurones. This was interpreted as specialization of the neural networks that control and co-ordinate the activity of vastly different effector systems in the two regions of the alimentary canal.

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Lucifer dyes—highly fluorescent dyes for biological tracing

Cited by 494 publications

References 39 publications

Synthesis and Photophysical Characterizations of Thermal -Stable Naphthalene Benzimidazoles

Synthesis and Photophysical Characterizations of Thermal -Stable Naphthalene Benzimidazoles

Excited-state dynamics of the fluorescent probe Lucifer Yellow in liquid solutions and in heterogeneous media

Electrical behaviour of myenteric neurones in the gastric corpus of the guinea‐pig.

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