Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs

Yamashita, Seiya; Mizota, Kashu; Okubo, Yusuke; Kageyama, Tatsuto; Yan, Lu; Fukuda, Junji

doi:10.1016/j.xpro.2022.101439

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…This characteristic enables relatively easy analysis of gene expression over time and in the same sample. Because of this advantage, bioluminescence-based gene expression analysis is used to monitor the temporal expression of circadian rhythm genes 3 and screen drugs that affect the expression of specific genes 4 .…”

Section: Introductionmentioning

confidence: 99%

Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila

Chihara

Itō²,

Matsuda³

et al. 2023

Preprint

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Bioluminescence generated by luciferase and luciferin has been extensively used in biological research. However, detecting signals from deep tissues in vivo poses a challenge to traditional methods. To overcome this, the Akaluc and AkaLumine bioluminescent systems were developed, resulting in improved signal detection. We evaluated the potential of Akaluc/AkaLumine in Drosophila melanogaster to establish a highly sensitive, non-invasive, and temporal detection method for gene expression. Our results showed that oral administration of AkaLumine to flies expressing Akaluc provided a higher luminescence signal than Luc/D-luciferin, with no observed harmful effects on flies. The Akaluc/AkaLumine system allows for monitoring of dynamic temporal changes in gene expression. Additionally, using the Akaluc fusion protein and fusion gene allows for protein level and mRNA splicing monitoring. Our findings indicate that the Akaluc/AkaLumine system is a powerful bioluminescence tool for analyzing gene and protein expression in deep tissues and small numbers of cells in Drosophila.

show abstract

Section: Introductionmentioning

confidence: 99%

Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila

Chihara

Itō²,

Matsuda³

et al. 2023

Preprint

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Advances in genomic toxicology: In vitro developmental toxicity test based on signal network disruption dynamics

Okubo,

Hirabayashi,

Fukuda

2024

Current Opinion in Toxicology

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Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila

Ito,

Matsuda,

Ukita

et al. 2023

Commun Biol

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Bioluminescence generated by luciferase and luciferin has been extensively used in biological research. However, detecting signals from deep tissues in vivo poses a challenge to traditional methods. To overcome this, the Akaluc and AkaLumine bioluminescent systems were developed, resulting in improved signal detection. We evaluate the potential of Akaluc/AkaLumine in Drosophila melanogaster to establish a highly sensitive, non-invasive, and temporal detection method for gene expression. Our results show that oral administration of AkaLumine to flies expressing Akaluc provided a higher luminescence signal than Luc/D-luciferin, with no observed harmful effects on flies. The Akaluc/AkaLumine system allows for monitoring of dynamic temporal changes in gene expression. Additionally, using the Akaluc fusion gene allows for mRNA splicing monitoring. Our findings indicate that the Akaluc/AkaLumine system is a powerful bioluminescence tool for analyzing gene expression in deep tissues and small numbers of cells in Drosophila.

show abstract

Luciferase assay system to monitor fibroblast growth factor signal disruption in human iPSCs

Cited by 3 publications

References 14 publications

Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila

Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila

Advances in genomic toxicology: In vitro developmental toxicity test based on signal network disruption dynamics

Akaluc/AkaLumine bioluminescence system enables highly sensitive, non-invasive and temporal monitoring of gene expression in Drosophila

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