Lucigenin-dependent chemiluminescence as a new assay for NAD(P) H-oxidase activity in particulate fractions of human polymorphonuclear leukocytes

Minkenberg, I.; Ferber, E.

doi:10.1016/0022-1759(84)90206-0

Cited by 152 publications

(64 citation statements)

References 22 publications

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“…However, it is known that luminol chemiluminescence rather reflects myeloperoxidase/H 2 O 2 /chIorite-dependent processes (19,20) whereas lucigenin chemiluminescence is more predictive for O 2 -dependent reactions (21,22). The luminol dependent chemiluminescence signal was indeed drastically reduced in the proband with complete myeloperoxidase deficiency.…”

Section: Discussionmentioning

confidence: 92%

Phagocytic Activity and Oxidative Burst of Granulocytes in Persons with Myeloperoxidase Deficiency

Gerber¹,

Kuçi²,

Zipfel³

et al. 1996

CCLM

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Summary:In the present study, phagocytosis and the oxidative metabolism of neutrophil granulocytes from five clinically healthy persons with different degrees of myeloperoxidase deficiency were investigated and compared to those of normal persons. The identification of individuals with myeloperoxidase deficiency was performed with the Bayer/Technicon H3 blood cell counter, which differentiates the leukocytes by measuring the peroxidase activity. Neutrophils of three out of five investigated myeloperoxidase deficient persons showed extremly low peroxidase indices (-53 and lower), but only the neutrophils of one person totally lacked myeloperoxidase. This was demonstrated by comparing myeloperoxidase mass concentration measured with an enzyme immunoassay, lack of HOC1 production, and was further confirmed by measuring luminol-and lucigenin-enhanced chemiluminescence. Characteristically, myeloperoxidase deficient granulocytes showed a strikingly decreased luminol-enhanced chemiluminescence while the lucigenin-enhanced chemiluminescence was significantly increased compared to normal granulocytes. Although there is a DNA sequence homology of about 70%, the activity of peroxidase in eosinophils was not affected in any myeloperoxidase deficient person investigated. Moreover, a person with a very rare defect of eosinophil peroxidase had completely normal myeloperoxidase activity. The lack of myeloperoxidase activity is compensated for by an increased phagocytic activity, an increased production of Superoxide anion (lucigeninchemiluminescence) and probably by an alternative metabolism of H 2 O 2 ; since persons lacking myeloperoxidase activity do not normally suffer from severe infections, H 2 O 2 is obviously metabolized to other reactive oxygen substrates than HOC1, e. g. to OH-radicals.

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Section: Discussionmentioning

confidence: 92%

Phagocytic Activity and Oxidative Burst of Granulocytes in Persons with Myeloperoxidase Deficiency

Gerber¹,

Kuçi²,

Zipfel³

et al. 1996

CCLM

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“…Luminol-dependent chemiluminescence (luminol-dependent CL) (Allen and Loose, 1976) and lucigenin-dependent chemiluminescence (lucigenin-dependent CL) (Minkenberg and Ferber, 1984) are alternative, cell permeable methods for detecting ROS production. Luminol is not sensitive to superoxide production (Faulkner and Fridovich, 1993) but may undergo a chemiluminescent oxidation with hydrogen peroxide (Littauer and Caraceni) and peroxynitrite (Kooy et al, 1997).…”

Section: Ros Detectionmentioning

confidence: 99%

Regulation and measurement of oxidative stress in apoptosis

Curtin

Donovan

Cotter

2002

Journal of Immunological Methods

518

351

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“…Previously, this method was shown to be specific for the detection of superoxide anion. [20][21][22] In each experiment, cells from individual animals were used. Cells obtained from one animal were used only once for control and stimulatory radical production (eg control, CALP1, PMA and CALP2; n ¼ 1).…”

Section: Generation Of Chemiluminescencementioning

confidence: 99%

Specific modulation of calmodulin activity induces a dramatic production of superoxide by alveolar macrophages

Broeke

Leusink-Muis

Hilberdink

et al. 2004

Laboratory Investigation

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Airway inflammation is a characteristic feature in airway diseases such as asthma and chronic obstructive pulmonary disease. Oxidative stress, caused by the excessive production of reactive oxygen species by inflammatory cells like macrophages, eosinophils and neutrophils, is thought to be important in the complex pathogenesis of such airway diseases. The calcium-sensing regulatory protein calmodulin (CaM) binds and regulates different target enzymes and proteins, including calcium channels. In the present study, we investigated whether CaM, via the modulation of calcium channel function, influences [Ca 2 þ ] i in pulmonary inflammatory cells, and consequently, modulates the production of reactive oxygen species by these cells. This was tested with a peptide termed calcium-like peptide 2 (CALP2), which was previously shown to regulate such channels. Specifically, radical production by purified broncho-alveolar lavage cells from guinea-pigs in response to CALP2 was measured. CALP2 was a strong activator of alveolar macrophages. In contrast, CALP2 was only a mild activator of neutrophils and did not induce radical production by eosinophils. The CALP2-induced radical production was mainly intracellular, and was completely blocked by the NADPH-oxidase inhibitor DPI, the superoxide inhibitor SOD and the CaM antagonist W7. Furthermore, the calcium channel blocker lanthanum partly inhibited the cellular activation by CALP2. We conclude that alveolar macrophages, but not neutrophils or eosinophils, can produce extremely high amounts of reactive oxygen species when stimulated via the calcium/CaM pathway. These results may contribute to new therapeutic strategies against oxidative stress in airway diseases.

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Lucigenin-dependent chemiluminescence as a new assay for NAD(P) H-oxidase activity in particulate fractions of human polymorphonuclear leukocytes

Cited by 152 publications

References 22 publications

Phagocytic Activity and Oxidative Burst of Granulocytes in Persons with Myeloperoxidase Deficiency

Phagocytic Activity and Oxidative Burst of Granulocytes in Persons with Myeloperoxidase Deficiency

Regulation and measurement of oxidative stress in apoptosis

Specific modulation of calmodulin activity induces a dramatic production of superoxide by alveolar macrophages

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