Lumenato protects normal human dermal fibroblasts from neutrophil-induced collagen-3 damage in co-cultures

Abstract

Collagen is the major structural protein in the extracellular matrix of skin produced by fibroblasts. UV exposure results in infiltration of neutrophils within the epidermis and dermis, inducing collagen damage and contributing to the process of photo-aging. Collagen-3 is an integral structural component with collagen-1, and is an important regulator of collagen-1 fibrillogenesis. Addition of neutrophils activated with TNFα to normal human dermal fibroblast cultures, but not their supernatant, caused significant collagen-3 damage. To study whether Lumenato can protect from collagen-3 damage, it was added to co-cultures of Normal human dermal fibroblasts and neutrophils activated with TNFα. Lumenato prevented collagen-3 damage induced by activated neutrophils in a dose-dependent manner in the co-cultures. Lumenato also induced a low rate of collagen-3 synthesis in a dose-dependent manner detected by pro-collagen-3 secretion, but did not affect fibroblast cell number. Although Lumenato inhibited MMP-8, MMP-9, and elastase secreted from neutrophils, its main effect was in inhibiting both NADPH oxidase-producing superoxides and MPO activity-producing halides in a dose-dependent manner that correlated with protection from collagen-3 damage. In conclusion, the results suggest that Lumenato induces low levels of collagen-3 that may contribute for skin health and is very effective in defending the co-cultures from collagen-3 damage by inhibiting free radicals secreted from neutrophils, thus, indicating Lumenato's possible potential for skin protection.

Effect of Lumenato oral supplementation on plasma carotenoid levels and improvement of visual and experiential skin attributes

“…As observed in this study, there was a consistent increase in plasma levels of carotenoids such as phytoene, phytofluene, and zeta‐carotene after ingesting Lumenato supplement. Carotenoids accumulate in skin where, besides promoting health by protecting from UV damage, they can provide cosmetic benefits by contributing to improved skin appearance and specific skin characteristics 37,55,56 …”

“…Carotenoids accumulate in skin where, besides promoting health by protecting from UV damage, they can provide cosmetic benefits by contributing to improved skin appearance and specific skin characteristics. 37,55,56 Phytoene has been reported to be a carotenoid with the highest bio-accessibility followed by phytofluene. 58 The dietary source that provides highest amounts of potentially absorbable phytoene/ phytofluene has been declared as tomato juice.…”

“…These include the quenching of hydroxyl radicals; activation of the antioxidant response element and thereby possible decreases in DNA damage in lymphocytes and reduction in inflammation 55 . Lumenato supplement has been shown to induce low levels of collagen‐3 that may contribute to skin protection by inhibiting free radicals secreted from neutrophils 56 . Phytoene and phytofluene absorb light maximally in the range of UV radiation (which explains why they appear colorless) protecting from both UVA‐ and UVB‐induced damage.…”

Effects of golden tomato extract on skin appearance—outlook into gene expression in cultured dermal fibroblasts and on trans‐epidermal water loss and skin barrier in human subjects

“…Specific benefits of lycopene and/or β‐carotene include protection against UV‐induced erythema and sun damage, 11‐13 reduced oxidative stress, 14 and antioxidant/anti‐inflammatory activities 15 . β‐Carotene is, of course, the precursor of vitamin A and is known to supports collagen synthesis 16,17 . Lycopene has been shown to protect against UV damage to cultured human dermal fibroblasts 4‐6 .…”

“…15 β-Carotene is, of course, the precursor of vitamin A and is known to supports collagen synthesis. 16,17 Lycopene has been shown to protect against UV damage to cultured human dermal fibroblasts. [4][5][6] In addition, it has been shown to suppress chemically induced skin cancer in an animal model.…”