Luminescence targeting and imaging using a nanoscale generation 3 dendrimer in an in vivo colorectal metastatic rat model

Abstract

Surgery is currently the best approach for treating either primary or metastatic hepatic malignancies. Since only 20% of patients with hepatic cancer are operable, regional therapies (RT) are emerging as alternate treatment modalities. However, RT's can have their own limitations at controlling tumor growth or lack the ability to detect such metastases. More can be done to enhance their efficacy. An animal model of hepatic metastases coupled with a gastroduodenal artery (GDA) cannulation technique may provide a site to apply such therapies. In our study, splenic injections were performed with CC531 adenocarcinoma cells, which generated metastatic hepatic tumors in WAG/RijHsd rats. Cannulation of GDA was achieved via a polyethylene catheter. Infusion of generation 3 polyamidoamine 4-amino-1,8-naphthalimide containing 8 europium ions (Eu-G3P4A18N) dendrimer via the GDA resulted in luminescence of the hepatic metastatic nodules. Imaging of the metastatic hepatic nodules was obtained with the help of a digital charge coupled device camera.

Assessing inter lanthanide photophysical interactions in co-doped titanium dioxide nanoparticles for multiplex assays

“…The luminescence of trivalent lanthanide cations (Ln 3+ ) is gaining increasing attention thanks to the unique characteristics of the core like nature of the 4f-4f transitions and nds use in biological imaging, bio-analytical applications, optoelectronics, telecommunications, lasers, sensing. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16] The Ln 3+ luminescence exhibits sharp emission bands spanning entire visible and near infrared (NIR) spectral range with minimum intra and inter Ln 3+ spectral overlap, longer lifetime (typically in the range of microseconds to milliseconds), and resistance to photobleaching. These properties offer possibilities for multiplexing, time-gated measurements and longer data acquisition; hence opening up avenues for selective and sensitive detection with better signal to noise ratio.…”

Lanthanide near infrared imaging in living cells with Yb 3+ nano metal organic frameworks

“…Their emission wavelengths are not affected by the environment, allowing them to be used in a broad range of conditions, including varied pH, and biological environments. Most luminescent lanthanide reporters are more resistant to photobleaching than organic fluorophores, which enables them to be used repeatedly and/or over long periods of time (7)(8)(9).…”

Click Modification in the N6 Region of A3 Adenosine Receptor-Selective Carbocyclic Nucleosides for Dendrimeric Tethering that Preserves Pharmacophore Recognition

“…50,51 Given the high A 3 AR affinity observed in the small molecular series, there is now a possibility of introducing radioisotopes to produce new radioligands for receptor characterization in vitro and in vivo. Prosthetic groups for targeting, therapeutic applications or diagnostic purposes, such as fluorescent reporter groups, might be introduced.…”