Luminescence thermometry for <i>in situ</i> temperature measurements in microfluidic devices

Geitenbeek, Robin G.; Vollenbroek, Jeroen C.; Weijgertze, Hannah M. H.; Trégouët, Corentin; Nieuwelink, Anne‐Eva; Kennedy, Chris L.; Weckhuysen, Bert M.; Lohse, Detlef; Blaaderen, Alfons van; Berg, Albert van den; Odijk, Mathieu; Meijerink, Andries

doi:10.1039/c8lc01292j

Cited by 82 publications

(72 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Temperature is an important control parameter that governs, e.g., the rate of chemical reactions, but also the optimum working efficiency of electronic devices or dynamics and viability of biological systems. As such, non-invasive, sensitive and remote temperature measurement techniques with the capability to spatially resolve temperature variations down to the micrometer range are becoming increasingly relevant [1][2][3][4][5][6][7]. Physiological temperature sensing is especially demanding in that regard as it even requires to accurately distinguish temperature fluctuations below 1 K. Luminescence nanothermometry is an appealing and rapidly emerging technique that meets those requirements and constantly improves [8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Making Nd3+ a Sensitive Luminescent Thermometer for Physiological Temperatures—An Account of Pitfalls in Boltzmann Thermometry

et al. 2020

Self Cite

View full text Add to dashboard Cite

Ratiometric luminescence thermometry employing luminescence within the biological transparency windows provides high potential for biothermal imaging. Nd3+ is a promising candidate for that purpose due to its intense radiative transitions within biological windows (BWs) I and II and the simultaneous efficient excitability within BW I. This makes Nd3+ almost unique among all lanthanides. Typically, emission from the two 4F3/2 crystal field levels is used for thermometry but the small ~100 cm−1 energy separation limits the sensitivity. A higher sensitivity for physiological temperatures is possible using the luminescence intensity ratio (LIR) of the emissive transitions from the 4F5/2 and 4F3/2 excited spin-orbit levels. Herein, we demonstrate and discuss various pitfalls that can occur in Boltzmann thermometry if this particular LIR is used for physiological temperature sensing. Both microcrystalline, dilute (0.1%) Nd3+-doped LaPO4 and LaPO4: x% Nd3+ (x = 2, 5, 10, 25, 100) nanocrystals serve as an illustrative example. Besides structural and optical characterization of those luminescent thermometers, the impact and consequences of the Nd3+ concentration on their luminescence and performance as Boltzmann-based thermometers are analyzed. For low Nd3+ concentrations, Boltzmann equilibrium starts just around 300 K. At higher Nd3+ concentrations, cross-relaxation processes enhance the decay rates of the 4F3/2 and 4F5/2 levels making the decay faster than the equilibration rates between the levels. It is shown that the onset of the useful temperature sensing range shifts to higher temperatures, even above ~ 450 K for Nd concentrations over 5%. A microscopic explanation for pitfalls in Boltzmann thermometry with Nd3+ is finally given and guidelines for the usability of this lanthanide ion in the field of physiological temperature sensing are elaborated. Insight in competition between thermal coupling through non-radiative transitions and population decay through cross-relaxation of the 4F5/2 and 4F3/2 spin-orbit levels of Nd3+ makes it possible to tailor the thermometric performance of Nd3+ to enable physiological temperature sensing.

show abstract

Section: Introductionmentioning

confidence: 99%

Making Nd3+ a Sensitive Luminescent Thermometer for Physiological Temperatures—An Account of Pitfalls in Boltzmann Thermometry

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nevertheless, contact thermometry, using a scanning probe, require long acquisition times and have limitations for measurements in wet samples and fluidic systems. Temperature sensing and imaging based on temperature-dependent fluorescent signals from a fluorescent reporter possess a high detection sensitivity and a superior temporal resolution; therefore, this is a promising and practical approach for thermometry at nano- and microscales in biology, nanobiotechnology, and nanomedicine fields 2–5 . This methodology simply requires a thermosensitive fluorescent material and an optical microscope or detector.…”

Section: Introductionmentioning

confidence: 99%

Highly cooperative fluorescence switching of self-assembled squaraine dye at tunable threshold temperatures using thermosensitive nanovesicles for optical sensing and imaging

Sou

Chan

Arai

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Thermosensitive fluorescent dyes can convert thermal signals into optical signals as a molecular nanoprobe. These nanoprobes are playing an increasingly important part in optical temperature sensing and imaging at the nano- and microscale. However, the ability of a fluorescent dye itself has sensitivity and accuracy limitations. Here we present a molecular strategy based on self-assembly to overcome such limitations. We found that thermosensitive nanovesicles composed of lipids and a unique fluorescent dye exhibit fluorescence switching characteristics at a threshold temperature. The switch is rapid and reversible and has a high signal to background ratio (>60), and is also highly sensitive to temperature (10–22%/°C) around the threshold value. Furthermore, the threshold temperature at which fluorescence switching is induced, can be tuned according to the phase transition temperature of the lipid bilayer membrane forming the nanovesicles. Spectroscopic analysis indicated that the fluorescence switching is induced by the aggregation-caused quenching and disaggregation-induced emission of the fluorescent dye in a cooperative response to the thermotropic phase transition of the membrane. This mechanism presents a useful approach for chemical and material design to develop fluorescent nanomaterials with superior fluorescence sensitivity to thermal signals for optical temperature sensing and imaging at the nano- and microscales.

show abstract

“…Section 3.1) [29,53,54] and UCNPs (cf. Section 3.3) [221] discussed in this review. Nonoptical methods do also exist, where the microfluidic channel can be printed on-top of a dedicated chips with known temperature gradients.…”

Section: Microfluidic and Surfacesmentioning

confidence: 99%

Optical Nanoscale Thermometry: From Fundamental Mechanisms to Emerging Practical Applications

Bradac

Lim

Chang

et al. 2020

Advanced Optical Materials

122

View full text Add to dashboard Cite

Knowledge of temperature and temperature gradients with nanoscale resolution is critical for a variety of applications in medicine, nanoelectronics, biology, and solid‐state‐based devices. The number of existing nanothermometry techniques is remarkably large, varying for materials, mechanisms, sensitivity, and operating ranges. In this work, a selected group of prominent nanoscale thermosensors is reviewed, which are all‐optical and nanoparticle‐based. Specifically, the focus is on the analysis of their fundamental mechanism to identify absolute, intrinsic capabilities and limitations of each nanothermometry platform. Prominent applications as well as future challenges and opportunities in the field are discussed.

show abstract

Luminescence thermometry for in situ temperature measurements in microfluidic devices

Abstract: In this work we present 3 showcases that luminescence thermometry is a promising and versatile technique for temperature monitoring in various microfluidic devices.

Cited by 82 publications

References 49 publications

Making Nd3+ a Sensitive Luminescent Thermometer for Physiological Temperatures—An Account of Pitfalls in Boltzmann Thermometry

Making Nd3+ a Sensitive Luminescent Thermometer for Physiological Temperatures—An Account of Pitfalls in Boltzmann Thermometry

Highly cooperative fluorescence switching of self-assembled squaraine dye at tunable threshold temperatures using thermosensitive nanovesicles for optical sensing and imaging

Optical Nanoscale Thermometry: From Fundamental Mechanisms to Emerging Practical Applications

Contact Info

Product

Resources

About