Nowadays nanostructures are in demand in various fields from biomedicine to green energy. Photoluminescence (PL) spectral measurements are a powerful tool to study nanomaterials unique physical and optical properties. Most modern spectral approaches are associated with the study of a sample on a substrate or in colloidal solution.In turn, we propose a technique for studying the luminescence of a single object levitating in a quadrupole Paul trap. To verify the technique, we investigate PL spectra of individual trapped charged microcluster of CdSe/ZnS quantum dots. The results obtained open prospects of optical research on single particles isolated from the environment.