Luminescent polystyrene latex nanoparticles doped with β-diketone europium chelate and methacrylic acid

Shin, Jong Dae; Lim, Woo Jin; Yu, Kwang Sik; Lee, Je‐Hun; Lee, Nam Seob; Jeong, Young‐Gil; Han, Seung‐Yun; Kim, Do Kyung

doi:10.1016/j.eurpolymj.2016.08.017

Cited by 6 publications

(4 citation statements)

References 27 publications

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“…Europium complexes [17,18], Eu(TFAAN) 3 (P(Oct) 3 ) 3 , was prepared based on previously reported method with a minor modification. Europium chloride hexahydrate (EuCl 3 ·6H 2 O, 0.366 g, 1 mmol) was dissolved in 50 mL distilled H 2 O.…”

Section: Methodsmentioning

confidence: 99%

Magnetofluorescent Nanocomposite Comprised of Carboxymethyl Dextran Coated Superparamagnetic Iron Oxide Nanoparticles and β-Diketon Coordinated Europium Complexes

Han

Lee

et al. 2019

Nanomaterials

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Red emitting europium (III) complexes Eu(TFAAN)3(P(Oct)3)3 (TFAAN = 2-(4,4,4-Trifluoroacetoacetyl)naphthalene, P(Oct)3 = trioctylphosphine) chelated on carboxymethyl dextran coated superparamagnetic iron oxide nanoparticles (CMD-SPIONs) was synthesized and the step wise synthetic process was reported. All the excitation spectra of distinctive photoluminesces were originated from f-f transition of EuIII with a strong red emission. The emission peaks are due to the hypersensitive transition 5D0→7F2 at 621 nm and 5D0→7F1 at 597 nm, 5D0→7F0 at 584 nm. No significant change in PL properties due to addition of CMD-SPIONs was observed. The cytotoxic effects of different concentrations and incubation times of Eu(TFAAN)3(P(Oct)3)3 chelated CMD-SPIONs were evaluated in HEK293T and HepG2 cells using the WST assay. The results imply that Eu(TFAAN)3(P(Oct)3)3 chelated CMD-SPIONs are not affecting the cell viability without altering the apoptosis and necrosis in the range of 10 to 240 μg/mL concentrations.

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Section: Methodsmentioning

confidence: 99%

Magnetofluorescent Nanocomposite Comprised of Carboxymethyl Dextran Coated Superparamagnetic Iron Oxide Nanoparticles and β-Diketon Coordinated Europium Complexes

Han

Lee

et al. 2019

Nanomaterials

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“…However, along with very high efficiencies of beads, including extinction coefficents of the complexes in excess of 50,000 M −1 ·cm −1 , a tremendous intensity increase is possible and observed. Thus, luminescent polymer beads comprising rare earth complexes have been developed in the most interesting size range of approximately 10 to a few hundred nm; as bead matrix materials predominantly polystyrene but also PMMA, or both, are employed [( Huhtinen et al, 2008 ; Aikawa et al, 2016 ; Shin et al, 2016 )]. Typically, the beads are synthesized first by micro-emulsion polymerization and then activated by incubation of the particles in rare earth solutions, although polymerization of the matrix in the presence of the complexes is also known ( Ando and Kawaguchi, 2005 ; Desbiens et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

“…Traditionally based on gold nanoparticles (as known from pregnancy tests, for example), rare earth polystyrene and PMMA particles (beads) are increasingly emerging as notably more sensitive substitutes ( Rundstrom et al, 2007 ; Juntunen et al, 2012 ; Ham et al, 2015 ; Song et al, 2015 ; Zhang et al, 2015 ; Shao et al, 2017 ; Salminen et al, 2019 ; Natarajan and Joseph, 2022 ) and are also applied to tackle the recent threat of COVID-19 ( Feng et al, 2020 ). Prominent complexes used are aromatic diketonates like tris(2-thenoyl-3,3,3-trifluoroacetone)Eu(III)-di(tri-n-octyl phosphine oxide), Eu(ttfa) 3 (TOPO) 2 ( Aikawa et al, 2016 ), and tris(1-(2-Naphthoyl)-3,3,3-trifluoroacetone)Europium(III)-di(tri-n-octyl phosphine oxide), Eu(ntfa) 3 (TOPO) 2 ( Huhtinen et al, 2008 ; Li et al, 2013 ; Shin et al, 2016 ); in these complexes the coordination of TOPO is granting high concentrations in the polymers.…”

Section: Introductionmentioning

confidence: 99%

High brightness red emitting polymer beads for immunoassays: Comparison between trifluoroacetylacetonates of Europium

et al. 2023

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Efficiently luminescing spherical polymer particles (beads) in the nanoscale regime of up to approximately 250 nm have become very valuable tools in bioanalytical assays. Eu3+- complexes imbedded in polymethacrylate and polystyrene in particular proved to be extraordinarily useful in sensitive immunochemical and multi-analyte assays, and histo- and cytochemistry. Their obvious advantages derive from both, the possibility to realize very high ratios of emitter complexes to target molecules, and the intrinsically long decay times of the Eu3+-complexes, which allows an almost complete discrimination against bothersome autofluorescence via time-gated measuring techniques; the narrow line emission in conjunction with large apparent Stokes shifts are additional benefits with regard to spectral separation of excitation and emission with optical filters. Last but not least, a reasonable strategy to couple the beads to the analytes is mandatory. We have thus screened a variety of complexes and ancillary ligands; the four most promising candidates evaluated and compared to each other were β-diketonates (trifluoroacetylacetonates, R-CO-CH-CO-CF3, R = - thienyl, -phenyl, -naphthyl and -phenanthryl); highest solubilities in polystyrene were obtained with trioctylphosphine co-ligands. All beads had overall quantum yields in excess of 80% as dried powders and lifetimes well beyond 600 µs. Core-shell particles were devised for the conjugation to model proteins (Avidine, Neutravidine). Their applicability was tested in biotinylated titer plates using time gated measurements and a Lateral Flow Assay as practical examples.

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“…The photoluminescence (PL) properties of Eu 3+ complex are strongly depending on constitutional composition of organic ligands. In addition, the carboxylic acid group of acrylic acid is strongly chelated with Eu 3+ than the Eu 3+ complex constituent molecules and protects the dissociation of Eu 3+ complex under complicated physiological conditions [ 28 ]. Based on the above theory, a study was reported in which carboxylic acid activated polystyrene-based copolymer nanoparticles were prepared and then synthesized fluorescent nanoparticles through coordination bonding of carboxylic acid and chelated Eu 3+ complex.…”

Section: Introductionmentioning

confidence: 99%

Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide Nanoparticles

et al. 2020

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Highly fluorescent magnetic nanoparticles (Eu(TTA)3(P(Oct)3)3@mSiO2@SPION) [europium (III) chloride hexahydrate = Eu; 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione = TTA; trioctylphosphine = (P(Oct)3); mesoporous silica = mSiO2; superparamagnetic iron oxide nanoparticle = SPION] were developed as a dual-functional imaging agent. The hierarchical structure was composed of a magnetic core and mesoporous silica shell was constructed using a cationic surfactant template after coating with phosphatidylcholine of oleic acid coated SPION. Afterward, the surface and cavities of mSiO2@SPION were modified with 3-(trimethoxysilyl) propyl methacrylate (TMSPMA) as a silane coupling agent to introduce methacrylate groups. Eu(TTA)3(P(Oct)3)3 molecules are penetrated, located and bonded covalently inside of the cavities/mesopores of mSiO2, it shows extremely stable anti-photobleaching properties. The emission spectra of Eu(TTA)3(P(Oct)3)3@mSiO2@SPION indicated typical hypersensitivity transition 5D0→7F2 at 621 nm. The concentration of Eu(TTA)3(P(Oct)3)3@mSiO2@SPION was varied between 10 and 500 μL/mL to evaluate the cytotoxicity with NCI-H460 (H460) cells using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. In addition, the presence of a strong red-emitting Eu(TTA)3(P(Oct)3)3@mSiO2@SPION in the cytoplasm was observed by fluorescence microscopy. Those results that it can be a potential candidate for dual-functional contrast agent and PL nanomaterials for fabricating the diagnostic kits to amplify the low signal.

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Luminescent polystyrene latex nanoparticles doped with β-diketone europium chelate and methacrylic acid

Cited by 6 publications

References 27 publications

Magnetofluorescent Nanocomposite Comprised of Carboxymethyl Dextran Coated Superparamagnetic Iron Oxide Nanoparticles and β-Diketon Coordinated Europium Complexes

Magnetofluorescent Nanocomposite Comprised of Carboxymethyl Dextran Coated Superparamagnetic Iron Oxide Nanoparticles and β-Diketon Coordinated Europium Complexes

High brightness red emitting polymer beads for immunoassays: Comparison between trifluoroacetylacetonates of Europium

Highly Luminescent and Anti-Photobleaching Core-Shell Structure of Mesoporous Silica and Phosphatidylcholine Modified Superparamagnetic Iron Oxide Nanoparticles

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