Luminex crossmatch for pretransplant evaluation of renal transplant recipients

Lal, Vandana; Mishra, Mahendra Narain

doi:10.1016/j.humimm.2015.07.054

Cited by 2 publications

(3 citation statements)

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“…Although DSAXM Class II false results have occasionally been reported [30][31][32][33] , in our study, the DSAXM results for HLA class II correlated well with FCXM results. Although our sample size was small, DSAXM was preferred to the CDCXM procedure, thereby eliminating the reliance on viable cells because DSAXM uses donor lysate, which can also be stored for future analysis.…”

Section: Discussionsupporting

confidence: 78%

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Evaluation of three different laboratory methods to detect preformed human leukocyte antigen antibodies in a South African kidney transplant population

et al. 2021

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Background: Anti-human leukocyte antigen antibodies (anti-HLA) play a crucial role in graft. Detection of anti-HLA, both pre- and post-transplant is a crucial investigation in clinical organ transplantation. Objectives: Three methodologies for the detection of lymphocytotoxic antibodies were compared to establish which of these is best suited to optimise pre-transplant donor-recipient matching. Methods: Serum samples from 15 renal transplant patients were tested for the presence of anti-HLA by i) cytotoxic-de- pendent cross-match (CDCXM), ii) flow cytometric cross-match (FCXM) and iii) Luminex-based donor specific antibody cross-match (DSAXM) method, Confirmatory tests for the presence of preformed HLA antibodies were tested using Lu- minex methodology. Results: Two (13%) of the 15 patients had positive HLA Class I antibodies (Ab) using all 3 methods. An additional 2 HLA Class I Ab were identified with FCXM/CDCXM. DSAXM identified 1 HLA Class I positive, not indicated by CDCXM/ FCXM. High HLA Class II positivity (40%), identified by CDCXM, while DSAXM and FCXM identified two and one patients, respectively. CDCXM produced 4 false-positive results confirmed by lymphocyte single antigen (LSA) assay. Conclusions: The DSAXM method appears to add value in pre-transplantation screening to identify pre-sensitised patients that may not reject the donor graft due to the absence of donor-specific antibodies. Keywords: Preformed human leukocyte; antigen antibodies; kidney transplant; population; South Africa.

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Section: Discussionsupporting

confidence: 78%

“…There have been reports by others of false-positive DSAXM Class II results [30][31][32][33] . However, the DSAXM Class II results recorded in the current study correlated well with FCXM, as well as with the Luminex DSA method.…”

Section: Limitation Of the Studymentioning

confidence: 99%

Evaluation of three different laboratory methods to detect preformed human leukocyte antigen antibodies in a South African kidney transplant population

et al. 2021

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“…Detection of DSA is useful because of its clarity of interpretation and low false positivity in post-transplant recipients. The authors however reported a high rate of false positive results in pretransplant work up [12]. False positive DSA results were confirmed on the basis of a negative PRA screen in eight recipients which was further confirmed in four of them because they were HLA identical with respect to donor for HLA DR. AMR including a combined picture with ACR, was histologically identified in 37/105 (35.2%) of biopsies of which 27 were positive for DSA.…”

Section: Discussionmentioning

confidence: 91%

Luminex Crossmatch as a Successful Algorithm for Post-Transplant Follow-up in Developing Nations-A Study of 169 Consecutive Samples

Mishra¹,

Lal²

2015

J Kidney

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Background: Since 2014 Luminex crossmatch (LXM) is being performed regularly in India for post-transplant management of renal allograft recipients. Aim: This study was planned to evaluate the possible role of the LXM in the post-transplant setting for the detection and characterization of HLA-specific IgG antibodies and to correlate with allograft biopsy and renal function. Methods: A retrospective study was performed on 169 consecutive LXM performed at a single center to investigate suspected allograft dysfunction (n=147) and for monitoring DSA in normally functioning allografts (n=22). A total of 116 biopsies (including 11 repeats) and 40 panel reactive antibody (PRA) screen tests were performed. Results: Donor specific antibodies (DSA) were detected in 81 samples (47.9%): 7 recipients had HLA class I DSA alone, 56 had only HLA class II DSA and 18 recipients had both. First time biopsies were categorized as: negative for rejection (n=30), antibody mediated rejection (n=32), acute cellular rejection (n=23), chronic rejection (n=15) and dual morphology (n=5). LXM was positive in 44/75 biopsies with rejection and transiently raised in 11/30 recipients who showed no evidence of rejection. On the basis of the LXM results biopsy was not performed in 42 recipients who showed improved renal function 15 recipients with non-specific findings on first biopsy. Conclusion: The study suggests that LXM is useful and economically viable algorithm for the detection of antibodies in the post-transplant work up of renal allograft recipients in developing nations.

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Luminex crossmatch for pretransplant evaluation of renal transplant recipients

Cited by 2 publications

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Evaluation of three different laboratory methods to detect preformed human leukocyte antigen antibodies in a South African kidney transplant population

Evaluation of three different laboratory methods to detect preformed human leukocyte antigen antibodies in a South African kidney transplant population

Luminex Crossmatch as a Successful Algorithm for Post-Transplant Follow-up in Developing Nations-A Study of 169 Consecutive Samples

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