Luminol chemiluminescence in micellar media

Hadjianestis, J.; Nikokavouras, J.

doi:10.1016/1010-6030(92)85232-j

Cited by 20 publications

(4 citation statements)

References 16 publications

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“…Many compounds increased or decrease luminol chemiluminescence (1-3) and these effects are exploited in quantitative analysis (4-7), in immunoassays (8-lo), in theoretical studies (3,11,12), and other applications.…”

Section: Ntrodu Ctlo Nmentioning

confidence: 99%

Enhancement and inhibition of luminol chemiluminescence by phenolic acids

Dı́az

Sánchez

García

1995

J. Biolumin. Chemilumin.

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We explored the behaviour of a series of phenolic acids used as enhancers or inhibitors of luminol chemiluminescence by three different methods to determine if behaviour was associated with phenolic acid structure and redox character. All the phenolic acids inhibited chemiluminescence when hexacyanoferrate (III) was reacted with the phenolic acids before adding luminol. The redox character of these compounds was clearly related to structure. When hexacyanoferrate(III)-luminol-O2 chemiluminescence was initiated by phenolic acid-luminol mixtures some phenolic acids behaved as enhancers of chemiluminescence, and others as inhibitors. We propose a mechanism to explain these findings. We found direct relationships between the redox character of the phenolic acids and the enhancement or inhibition of the chemiluminescence of the luminol-H2O2-peroxidase system and we propose mechanism to explain these phenomena.

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Section: Ntrodu Ctlo Nmentioning

confidence: 99%

Enhancement and inhibition of luminol chemiluminescence by phenolic acids

Dı́az

Sánchez

García

1995

J. Biolumin. Chemilumin.

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“…In the presence of hydrogencarbonate, the effect of the surfactant on the CL reaction was investigated.2a,2', 28 The surfactants examined were nonionic {TritonX-100 (polyethylene glycol mono-4-octylpheny ether) and Brij 35 (polyoxyethylene-23-lauryl ether)}, cationic {Zephir-amine (benzyldimethyltetradecylammonium chloride), DTAB (dodecyltrimethyl ammonium bromide) and CTAB (cetyltrimethyl ammonium bromide)} and anionic surfactants {SDS (sodium dodecyl sulfate) and SDBS (sodium dodecylbenzenesulfonate)}.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Flow Injection Determination of Acetylcholine and Choline Based on Luminol Chemiluminescence in a Micellar System with On-Line Dialysis

1997

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A simultaneous flow injection method (FIA) was developed for the determination of acetylcholine and choline. This method is based on the determination of hydrogen peroxide (H2O2) produced from acetylcholine and choline by enzyme reactions. The determination of H2O2 was carried out by Co(II)-catalyzed luminol chemiluminescence (CL) reaction. The addition of sodium dodecylsulfate (SDS) in this CL system enhanced the CL intensity and sodium hydrogencarbonate showed an activating effect on the catalysis of Co(II) on the CL reaction. Interference of albumin for the CL reaction was removed by introducing an on-line dialysis unit and anion exchange column in the FIA system. A 10-6 mol dm-3 level of acetylcholine and choline can be determined at a rate of 6 samples per hour (12 peaks). The method was successfully applied to the determination of acetylcholine and choline in a culture medium. KeywordsFlow injection analysis, micelle-enhanced esterase, choline oxidase, Co(II) catalysis luminol chemiluminescence, enzyme reaction, acetylcholineAcetylcholine is one of the neurotransmitters in the central and peripheral nervous systems and is converted to choline as a metabolite with the action of acetylcholine esterase (AChE). Then the choline produced is successively hydrolyzed by choline oxidase (ChO) to betaine and hydrogen peroxide (H2O2), consuming any dissolved oxygen in the sample solution. Recently pharmacological effects of acetylcholine and choline were reported in connection with the development of medication for Alzheimer-type senile dementia 1 Therefore, the determination of trace amounts of acetylcholine and choline has considerable importance in medical research and clinical diagnoses. Various methods for acetylcholine and choline have been reported, such as HPLC2-5, spectrophotometry6, electrochemical method including enzyme sensor'_12, peroxyoxalate CL method13, GC/MS 14, LC/MS15 and radioenzymatic method.16,17 However, these methods are laborious, time-consuming, lack sensitivity and need expensive apparatus. A simple and sensitive method for both substrates is thus required.On the other hand, the CL method has been known as a sensitive and simple one for the determination of H2O2 and various biological substances)8 The present paper describes the simultaneous flow injection analysis (FIA) of acetylcholine and choline based on the luminol CL reaction in a micellar system with on-line dialysis19-21, and on the determination of H2O2 produced from acetylcholine and choline by enzyme reactions according to Eqs. (1) and (2).In the determination of acetylcholine, coexisting choline was removed after conversion to H2O2 by reaction (2), followed by the decomposition of H2O2 to oxygen by the action of catalase (CAT) according toThen acetylcholine was subsequently hydrolyzed to H2O2 via Eqs.(1) and (2). In the determination of choline, only the second enzyme reaction (Eq. (2)) was used. The concentrations of H2O2 produced from acetylcholine and choline were successively determined by Co(II)-catalyzed luminol CL reacti...

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“…Most of the published literature relates to observed CL enhancement from the luminol reaction using the two surfactants CTAB and CTAC. [21][22][23] These reagents were therefore selected and checked to ensure they enabled electroosmotic flow in the micro reactor. It was impossible to optimise all factors at once on the micro reactor because there were too many variables and a high level of CL emission was required before it could be detected in the unoptimised micro reactor system.…”

Section: Electrokinetically Pumped CL Systemmentioning

confidence: 99%

An investigation of electroosmotic flow and pressure pumped luminol chemiluminescence detection for cobalt analysis in a miniaturised total analytical systemElectronic Supplementary Information available. See http://www.rsc.org/suppdata/lc/b1/b107061d/

2001

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The luminol chemiluminescence reaction has been investigated for the determination of cobalt in a planar glass micro reactor. Reagents were mobilised within an etched glass chip with 200 microm wide channels using either electrically driven flow with voltages below 200 V cm(-1) or negative pressure pumping. Surfactants were used to assist mobilisation of the luminol for electrically driven flow and this also increased the chemiluminescence intensity in that system. A calibration over seven orders of magnitude was achieved for cobalt(II) nitrate with the negative pressure pumping system, and the limit of detection at the 95% confidence level was 3 x 10(-11) mol l(-1). The limit of detection for the electrokinetic flow was 4 x 10(-11) mol l(-1) and the linear range was between 10(-10) to 10(-6) mol l(-1). The advantages and disadvantages of both mobilisation methods are discussed for this reaction.

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Luminol chemiluminescence in micellar media

Cited by 20 publications

References 16 publications

Enhancement and inhibition of luminol chemiluminescence by phenolic acids

Enhancement and inhibition of luminol chemiluminescence by phenolic acids

Simultaneous Flow Injection Determination of Acetylcholine and Choline Based on Luminol Chemiluminescence in a Micellar System with On-Line Dialysis

An investigation of electroosmotic flow and pressure pumped luminol chemiluminescence detection for cobalt analysis in a miniaturised total analytical systemElectronic Supplementary Information available. See http://www.rsc.org/suppdata/lc/b1/b107061d/

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