Luminol electrochemiluminescence with screen-printed electrodes for low-cost disposable oxidase-based optical sensors

Leca, Béatrice; Blum, Loı̈c J.

doi:10.1039/b002284p

Cited by 74 publications

(29 citation statements)

References 13 publications

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“…Furthermore, direct signal transduction of the molecular recognition events appears more relevant from a biomimetic point of view. With the double aim of miniaturising and directly interfacing the biomimetic sensing layer with an efficient signal transducer, the AChE-IgG-glycolipid complex has been associated with an optical device previously developed in our group for choline detection [35] ( Figure 5). Briefly, choline oxidase (ChOD) is entrapped in a photopolymer on a screen-printed electrode maintained at a potential allowing oxidation of luminol present in solution.…”

Section: Association Of a Langmuir-blodgett Nanostructure With An Elementioning

confidence: 99%

“…The peptide signal and the propeptide, cleaved in the mature enzymes, are highlighted in blue and green, respectively. Electrochemiluminescent detection of the activity of immobilised AChE: The experimental ECL system, previously designed in our group, has been described elsewhere, [35] while Figure 5 depicts a schematic representation of the ECL system used. Briefly, it consists of a screen-printed electrode (4 0.6 cm) with a working electrode area of 0.18 cm 2 connected to a potentiostat (PRGE, TacusselRadiometer, Villeurbanne, France) and placed at 25 8C in a glass cuvette protected from ambient light.…”

Section: à2mentioning

confidence: 99%

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Kinetics Study of Bungarus fasciatus Venom Acetylcholinesterase Immobilised on a Langmuir–Blodgett Proteo‐Glycolipidic Bilayer

et al. 2005

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This study deals with the kinetics properties of an enzyme immobilised in a defined orientation in a biomimetic environment. For this purpose, acetylcholinesterase (AChE) was captured at the surface of a nanostructured proteo-glycolipidic Langmuir-Blodgett film through specific recognition by a noninhibitor monoclonal antibody (IgG) inserted in a neoglycolipid bilayer. Modelling of this molecular assembly provided a plausible interpretation of the functional orientation of the enzyme. The AChE activity being stable for several weeks, the enzyme kinetics were investigated, and fitted perfectly with heterogeneous biocatalytic behaviour representative of cellular enzymatic catalysis. The AChE-IgG-glycolipid nanostructure was directly interfaced with an efficient optical device. Such an association, leading to an intimate contact between the nanostructure and the biochemical signal transducer, gives direct access to the intrinsic AChE behaviour. This study thus demonstrates the potential for direct investigation of the kinetic behaviour of an immobilised enzyme on a lipid bilayer through an efficient transduction system.

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Section: Association Of a Langmuir-blodgett Nanostructure With An Elementioning

confidence: 99%

Section: à2mentioning

confidence: 99%

Kinetics Study of Bungarus fasciatus Venom Acetylcholinesterase Immobilised on a Langmuir–Blodgett Proteo‐Glycolipidic Bilayer

et al. 2005

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“…In addition to these benefits, the current widespread interest in screen printing arises from the availability of a wide selection of methods with which the electrode can be modified. Different ink compositions can be produced by either the addition or deposition of various substances such as; enzymes [14], polymers [18], and metals [19,20]. A major drawback with screen printing is that they are usually used in a batch system [14,21] and incorporating them into a flow system has always been a crucial challenge as the following criteria must be met: the cell needed be designed to incorporate (or allow the simple exchange) of the electrodes with good mechanical stability and precise alignment.…”

Section: Introductionmentioning

confidence: 99%

“…Over the last two decades screen printed electrodes (SPE) have gained considerable attention in many electrochemical [10 -13] and ECL [14,15] systems for use in disposable applications. This is a result of their numerous advantages, including: low cost, excellent reproducibility, and ease of miniaturization [16].…”

Section: Introductionmentioning

confidence: 99%

Hybrid Microfluidic Sensors Fabricated by Screen Printing and Injection Molding for Electrochemical and Electrochemiluminescence Detection

et al. 2009

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An integrated microfluidic/electrochemical sensor with a dual working electrode configuration was fabricated for voltammetric and electrochemiluminescence (ECL) applications. The fabrication method integrates the two techniques of injection molding and screen printing for the first time. Injection molding is used to fabricate the flow cell with integrated electrical and fluidic connectors. Screen printing was used to print the electrodes directly onto the injection molded base plate. An over molding procedure followed by ultrasonic welding was used to form the complete sensor. The electrochemical and ECL performance of the sensors was assessed by applying different electrochemical methods such as cyclic voltammetry (CV), anodic stripping voltammetry (ASV) and chronoamperometry. The attractive analytical performance demonstrates good capability of the disposable sensors for routine online electrochemical and ECL measurements.

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“…In a first approach, the electrochemiluminescence of luminol was triggered using a glassy carbon electrode (GCE). Afterwards, miniaturization of electrochemiluminescencebased sensors was achieved using screen-printed electrodes instead of glassy carbon macroelectrodes (11). The main characteristics of these electroluminescence-based biosensors and their use with real samples are presented.…”

Section: Introductionmentioning

confidence: 99%

Electrogenerated chemiluminescence of luminol for oxidase‐based fibre‐optic biosensors

2001

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The luminol electrochemiluminescence has been exploited for the development of several fibre-optic biosensors allowing the detection of hydrogen peroxide and of substrates of H(2)O(2)-producing oxidases. Electro-optical flow injection analysis of glucose, lactate, cholesterol and choline are thus described. To perform the experiments, a glassy carbon electrode was polarized at a fixed potential. Luminol was then electrochemically oxidized and could react in the presence of hydrogen peroxide to produce light. Several parameters had to be optimized to obtain reliable optical biosensors. An optimum applied potential of +425 mV between the glassy carbon electrode and the platinum pseudo-reference electrode was determined, allowing the best signal: noise ratio to be obtained. It was also necessary to optimize the experimental conditions for the immobilization of the different oxidases involved (preactivated membranes, chemically activated collagen membranes, photopolymerized matrix). For each biosensor developed, the optimum reaction conditions have been studied: buffer composition, pH, temperature, flow rate and luminol concentration. Under optimal conditions, the detection limits (S/N = 3) were 30 pmol, 60 pmol, 0.6 nmol and 10 pmol for lactate, glucose, cholesterol and choline, respectively. The miniaturization of electrochemiluminescence-based biosensors has been realized using screen-printed electrodes instead of a glassy carbon macroelectrode, with choline oxidase as a model H(2)O(2)-generating oxidase.

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Luminol electrochemiluminescence with screen-printed electrodes for low-cost disposable oxidase-based optical sensors

Cited by 74 publications

References 13 publications

Kinetics Study of Bungarus fasciatus Venom Acetylcholinesterase Immobilised on a Langmuir–Blodgett Proteo‐Glycolipidic Bilayer

Kinetics Study of Bungarus fasciatus Venom Acetylcholinesterase Immobilised on a Langmuir–Blodgett Proteo‐Glycolipidic Bilayer

Hybrid Microfluidic Sensors Fabricated by Screen Printing and Injection Molding for Electrochemical and Electrochemiluminescence Detection

Electrogenerated chemiluminescence of luminol for oxidase‐based fibre‐optic biosensors

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