Luminol enhanced chemiluminescence of the perfused rat heart during ischemia and reperfusion

Kumar, Chellappa; Okuda, Masahiro; Ikai, Iwao; Chance, Britton

doi:10.1016/0014-5793(90)80463-s

Cited by 30 publications

(9 citation statements)

References 21 publications

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“…Light emission was reduced to normal values in presence of SOD. This was also observed by Kumar et al (1990), who studied the CL response of the perfused rat heart during ischemia and reperfusion. No light emission was observed in absence of cells, indicating that the CL was due to the respiratory burst of the neutrophils.…”

Section: Results Obtained With the Cf-testsupporting

confidence: 66%

Clastogenic Factors as Potential Biomarkers of Increased Superoxide Production

Emerit

2007

Biomark�Insights

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The formation of clastogenic factors (CF) and their damaging effects are mediated by superoxide, since superoxide dismutase is regularly protective. CF are produced via superoxide and stimulate the production of superoxide by monocytes and neutrophils. This results in a selfsustaining and longlasting process of clastogenesis, which may exceed the DNA repair system and ultimately lead to cancer (Emerit, 1994). An increased cancer risk is indeed observed in conditions accompanied by CF formation. These include irradiated persons, patients with chronic inflammatory diseases, HIV-infected persons and the chromosomal breakage syndromes ataxia telangiectasia, Bloom’s syndrome and Fanconi’s anemia. Biochemical analysis has identified lipid peroxidation products, arachidonic acid metabolites, nucleotides of inosine and cytokines, in particular tumor necrosis factor alpha, as the clastogenic and also superoxide stimulating components of CF. Due to their chromosome damaging effects, these oxidants can be detected with classical cytogenetic techniques. Their synergistic action renders the CF-test particularly sensitive for the detection of a pro-oxidant state. Correlations were observed between CF and other biomarkers of oxidative stress such as decreases in total plasma thiols or increases in TBARS or chemiluminescence. Correlations between CF and disease activity, between CF and radiation exposure, suggest the study of CF for monitoring these conditions. CF may also be useful as biochemical markers and intermediate endpoints for the evaluation of promising antioxidant drugs.CF formation represents a link between chronic inflammation and carcinogenesis. Prophylactic use of superoxide scavengers as anticarcinogens is therefore suggested.

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Section: Results Obtained With the Cf-testsupporting

confidence: 66%

Clastogenic Factors as Potential Biomarkers of Increased Superoxide Production

Emerit

2007

Biomark�Insights

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“…In addition to Ca 2+ -mediated reduction in mitochondrial ATP production, aberrations in mitochondrial electron chain functioning can result in 662 M. Arundine and M. Tymianski Glutamate toxicity in ischemia and traumatic brain injury excessive ROS production, leading to neurotoxicity [121][122][123][124]. Increased superoxide anion production (O 2 -) has been documented in both ischemic insults and traumatic brain injury [125]. ROS are also produced in the neuronal cytoplasm following elevations in intracellular Ca 2+ .…”

Section: Role Of Ros In Executing Glutamate Neurotoxicitymentioning

confidence: 99%

Molecular mechanisms of glutamate-dependent neurodegeneration in ischemia and traumatic brain injury

Arundine

Tymianski

2004

Cellular and Molecular Life Sciences (CMLS)

576

407

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Stroke and neurotrauma mediate neuronal death through a series of events that involve multiple interdependent molecular pathways. It has been suggested that these pathways are triggered following elevations in extracellular excitatory amino acids, primarily glutamate [1]. This report outlines mechanisms involving glutamate-mediated excitotoxicity with specific focus on (i) the role of Ca(2+) in neurotoxicity, (ii) The concept of 'source specificity' of neurotoxicity, (iii) the role of the ionotropic N-methyl-D-aspartate (NMDA)-subtype glutamate receptor and its associated submembrane molecules that may give rise to signaling specificity in excitotoxicity and (iv) the role of glutamate-mediated free-radical generation and associated cell death pathways. We also highlight a novel, peptide-based approach for uncoupling NMDA receptors from excitotoxicity in the rat central nervous system subjected to focal ischemia, thereby reducing stroke infarct volume and improving neurological functioning.

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“…Reperfusion is not a benign process. It involves release of reactive oxygen radicals [21], increase in intracellular calcium via Na + /Ca 2+ exchange [22], and realkalinization of the cellular milieu [23]. This also involves the cell signaling pathways described above and, with the restoration of oxygenation, ATP synthesis can restart allowing a further round of apoptosis [24].…”

Section: Reperfusion Injurymentioning

confidence: 97%

The Role of Minocycline in Ischemia-Reperfusion Injury: A Comprehensive Review of an Old Drug with New Implications

Ulgen¹,

Field²,

Qureshi³

et al. 2011

PRC

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Minocycline is a semi-synthetic tetracycline that inhibits bacterial protein synthesis and hence is used for the treatment of many infectious diseases. Over the years, many other interesting properties of minocycline have been identified and been used to make patents which include anti-inflammatory, anti-apoptotic, matrix metalloproteinase inhibitor and free oxygen radical scavenger activity. Ischemia-reperfusion injury is a concern for almost every clinical specialty and minocycline seems to be an attractive cytoprotective agent that can ameliorate the damage due to these properties. Ischemia-reperfusion injury is a complex process and involves various pathways that lead to cell death. This review focuses on the body of evidence describing various proposed mechanisms of action of minocycline and its current experimental use in various animal models of ischemia-reperfusion injury.

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Luminol enhanced chemiluminescence of the perfused rat heart during ischemia and reperfusion

Cited by 30 publications

References 21 publications

Clastogenic Factors as Potential Biomarkers of Increased Superoxide Production

Clastogenic Factors as Potential Biomarkers of Increased Superoxide Production

Molecular mechanisms of glutamate-dependent neurodegeneration in ischemia and traumatic brain injury

The Role of Minocycline in Ischemia-Reperfusion Injury: A Comprehensive Review of an Old Drug with New Implications

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