Luminol–hydrogen peroxide–horseradish peroxidase chemiluminescence intensification by kosmotrope ammonium sulfate

Abstract

The kosmotropic effect induced by ammonium sulfate (AS) at concentrations greater than approximately 2.8 M allows the marked intensification of chemiluminescence (CL) arising from a conventional luminol–hydrogen peroxide (H2O2)–horseradish peroxidase (HRP) reaction. Because of the kosmotropic effect, CL is intensified by at least three orders of magnitude than that from the conventional HRP-catalyzed luminol reaction with no AS; the linear relationship between the CL intensity and the HRP concentration is established over the range of 0.3 pM to several tens of pM. The novel CL intensification effect on the HRP-catalyzed luminol CL can be stably and reproducibly induced. Graphical abstract

RETRACTED: Development of a highly sensitive chemiluminescence immunoassay using a novel signal-enhanced detection system for quantitation of durvalumab, an immune-checkpoint inhibitor monoclonal antibody used for immunotherapy of lung cancer

“…This increased generation of CL is known as enhanced CL and several CL enhancers are available for the HRP-luminol-H 2 O 2 reaction. Enhanced CL HRP-luminol-H 2 O 2 reaction has been applied widely for the detection of various substances [ [21] , [22] , [23] , [24] ]. Previous studies revealed that the use of 4-(1,2,4-triazol-1-yl)phenol (TRP) as a CL enhancer provided more intense, prolonged, and stable light emissions in developing CLIA [ 25 ].…”

Development of indirect competitive ELISA and CLEIA for quantitative analysis of melatonin in health products

“…4), which is ascribed to the superiority of HRP-luminol-H 2 O 2 chemiluminescence as a detection system. 28,29 Both methods showed higher sensitivity and lower detection limits compared to previously reported immunoassays (Table 2). Furthermore, there was no cross-reaction with structural analogues of MT (CR% ≤ 0.001%, Table S1†).…”

A novel ultrasensitive chemiluminescence enzyme immunoassay by employment of a signal enhancement of horseradish peroxidase-luminol-hydrogen peroxide reaction for the quantitation of atezolizumab, a monoclonal antibody used for cancer immunotherapy

“…Various compounds have been utilized as signal enhancers in the development of HRP-based CLEIAs, resulting in enhancement of CL and yield more long-lasting, and stable light emission. [26][27][28] Previous research 29 has demonstrated that 4-(imidazol-1-yl)phenol (IMP) as a CL enhancer exhibits enhanced light emissions that are more intense, prolonged, and stable when developing CLEIAs. Therefore, the HRP-luminol-H 2 O 2 -IMP reaction was chosen and implemented as the detection system in the CLEIA described in this article.…”